Log In Sign up
The largest database of trusted experimental protocols

Annexin 5

Manufactured by Roche
Visit Supplier
Sourced in Germany, United States, Switzerland

Annexin V is a laboratory reagent used to detect and quantify apoptosis, a form of programmed cell death. It functions by binding to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis.

Automatically generated - may contain errors

Lab products found in correlation

Propidium iodide, by Roche (3 mentions) Propidium iodide, by Merck Group (3 mentions) Annexin 5, by BD (3 mentions) Dabrafenib, by Euromedex (2 mentions) Facscalibur, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Trypan blue, by Thermo Fisher Scientific (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Plx4032, by Euromedex (2 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (2 mentions) Facscalibur flow cytometer, by BD (2 mentions) Vemurafenib, by Euromedex (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Bms 345541, by Merck Group (1 mentions) Anti jarid1b, by Cell Signaling Technology (1 mentions) Trametinib, by Euromedex (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Tnf α, by Cell Signaling Technology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions)

39 protocols using annexin 5

1

Apoptosis Quantification by FACS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Trypan blue exclusion was used to assess cell viability. The induction of apoptosis was quantified by fluorescence-activated cell sorting (FACS) on treated cells stained with annexin V. Briefly, cells were washed, resuspended with annexin V binding buffer, stained with fluorescein isothiocyanate (FITC)–conjugated annexin V (Roche, Mannheim, Germany) for 15 min at room temperature in the dark, and then washed and counterstained with propidium iodide (PI). The analysis was performed by a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ) at a wavelength of 488 nm using Cell QuestPro Software (Beckman-Coulter, Fullerton, CA).
Frolova O., Benito J., Brooks C., Wang R.Y., Korchin B., Rowinsky E.K., Cortes J., Kantarjian H., Andreeff M., Frankel A.E, & Konopleva M. (2014). SL-401 and SL-501, Targeted Therapeutics Directed at the Interleukin-3 Receptor, Inhibit the Growth of Leukaemic Cells and Stem Cells in Advanced Phase Chronic Myeloid Leukaemia. British journal of haematology, 166(6), 862-874.
+ Open protocol
+ Expand
2

Apoptosis Assay in HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HeLa cells were seeded at 2 × 105 cell /well in 6-well plates and irradiation with gamma-ray. After 24 h, the cells were fixed with cold ethanol and then stained with propidium iodide according to kit manuscript (MultiSciences). The cells (about 20,000) were analyzed by flow cytometry (Beckman CytoFLEX, USA). The total cells were washed once and resuspended in Annexin V binding buffer, and then stained with 5 μl Annexin V and 5μl PI (Roche, Germany) for 20 min and analyzed with Beckman CytoFLEX flow cytometry. Ten thousand events were collected for each sample.
Shao C.S., Zhou X.H., Miao Y.H., Wang P., Zhang Q.Q, & Huang Q. (2021). In situ observation of mitochondrial biogenesis as the early event of apoptosis. iScience, 24(9), 103038.
+ Open protocol
+ Expand
3

Apoptosis Induction in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with various doses of GCS-100 (La Jolla Pharmaceutical Company), and/or ABT-737 and/or ABT-199 (all from Selleck Chemicals, Houston, TX) for various times. Cell death was assessed by trypan blue staining. To determine that the mechanism of cell death was apoptosis, cells were stained with Annexin V (Roche Diagnostics, Indianapolis, IN) / 4',6-diamidino-2-phenylindole (DAPI) and the percentages of apoptotic cells were assessed by flow cytometry. Apoptotic cells were identified as positive for Annexin V staining using a Becton Dickinson LSR II flow cytometer (Becton Dickinson, San Jose, CA). Total viable cells were determined by total Annexin V negative/DAPI negative cells with counting beads (Roche) included to determine total cell number.
Ruvolo P.P., Ruvolo V.R., Benton C.B., AlRawi A., Burks J.K., Schober W., Rolke J., Tidmarsh G., Hail N J.r., Davis R.E, & Andreeff M. (2015). Combination of galectin inhibitor GCS-100 and BH3 mimetics eliminates both p53 wild type and p53 null AML cells. Biochimica et biophysica acta, 1863(4), 562-571.
+ Open protocol
+ Expand
4

Signaling Pathway Modulation in Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMS-345541 was purchased from Sigma (Saint-Quentin-Fallavier, France). Vemurafenib, dabrafenib and trametinib were purchased from Euromedex (Souffelweyersheim, France) and TNFα was from PeproTech (Neuilly-Sur-Seine, France). Antibodies against CD271 was purchased from BD Biosciences (Franklin Lakes, NJ, USA), anti-ERK(D-2); anti-cleaved-PARP, anti-cleaved caspase-3, anti-pERK, anti-caspase-3, anti-JARID1B and TNFα blocking antibody were from Cell Signaling Technology Inc. (Beverly, MA, USA). Anti-IKK and anti-IκBα monoclonal antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-ABCB5 monoclonal antibody was purchased from Novus Biologicals (Abingdon, UK). Annexin V was purchased from Roche Diagnostic corporation (Indianapolis, IN, USA).
Lehraiki A., Cerezo M., Rouaud F., Abbe P., Allegra M., Kluza J., Marchetti P., Imbert V., Cheli Y., Bertolotto C., Ballotti R, & Rocchi S. (2015). Increased CD271 expression by the NF-kB pathway promotes melanoma cell survival and drives acquired resistance to BRAF inhibitor vemurafenib. Cell Discovery, 1, 15030-.
+ Open protocol
+ Expand
5

Apoptosis Assay for Drug Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
After treated with different drug formulation (DOX 2 μM and ATO 4 μM) for 24 h, cells were collected and washed twice with PBS. Then cells were stained with 7-aminoactinomycin D (7-AAD, Invitrogen) and Annexin-V (Roche) at room temperature for 20 min in dark. The fluorescence was detected by flow cytometry, and the data was analyzed by Flowjo software.
Liu H., Zhang Z., Chi X., Zhao Z., Huang D., Jin J, & Gao J. (2016). Arsenite-loaded nanoparticles inhibit PARP-1 to overcome multidrug resistance in hepatocellular carcinoma cells. Scientific Reports, 6, 31009.
+ Open protocol
+ Expand
6

Flow Cytometry Analysis of Apoptosis Induced by Fe3O4@Glu-Safranal NPs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry assay was used to investigate the effect of exposure to Fe3O4@Glu-Safranal NPs on the population of apoptotic and necrotic cells in the liver cancer cell line. For this purpose, HepG2 cells were treated with a 50% inhibitory concentration of nanoparticles for 24 h and then, the cells were stained by propidium iodide and Annexin V (Roche, Germany). Untreated cells were also considered as the negative control. Finally, the population of apoptotic and necrotic cells was quantified by the Partec™ flow cytometry instrument (Germany).
Mikaeili Ghezeljeh S., Salehzadeh A, & Ataei-e Jaliseh S. (2024). Iron oxide nanoparticles coated with Glucose and conjugated with Safranal (Fe3O4@Glu-Safranal NPs) inducing apoptosis in liver cancer cell line (HepG2). BMC Chemistry, 18(1), 33.
+ Open protocol
+ Expand
7

Evaluating BAFF-Induced B Cell Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF (R&D Systems) for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD; Sigma-Aldrich).
Dobenecker M.W., Marcello J., Becker A., Rudensky E., Bhanu N.V., Carrol T., Garcia B.A., Prinjha R., Yurchenko V, & Tarakhovsky A. (2020). The catalytic domain of the histone methyltransferase NSD2/MMSET is required for the generation of B1 cells in mice. FEBS letters, 594(20), 3324-3337.
+ Open protocol
+ Expand
8

Viability Assay for Activated PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly isolated PBMCs were stimulated with 1 μg/mL PHA overnight. Pre-activated PBMCs were stimulated with IL-2 containing molecules for 4 d before the anti-Fas antibody (clone CH11, Millipore) was added for additional 16 h. The percentage of living cells was measured by using Annexin V (Roche Applied Science) and the LIVE/DEAD Fixable Viability Stain eFluor 660 (eBioscience).
Klein C., Waldhauer I., Nicolini V.G., Freimoser-Grundschober A., Nayak T., Vugts D.J., Dunn C., Bolijn M., Benz J., Stihle M., Lang S., Roemmele M., Hofer T., van Puijenbroek E., Wittig D., Moser S., Ast O., Brünker P., Gorr I.H., Neumann S., de Vera Mudry M.C., Hinton H., Crameri F., Saro J., Evers S., Gerdes C., Bacac M., van Dongen G., Moessner E, & Umaña P. (2017). Cergutuzumab amunaleukin (CEA-IL2v), a CEA-targeted IL-2 variant-based immunocytokine for combination cancer immunotherapy: Overcoming limitations of aldesleukin and conventional IL-2-based immunocytokines. Oncoimmunology, 6(3), e1277306.
+ Open protocol
+ Expand
9

Apoptosis Measurement in MN-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MN-1 cells were seeded on 6-well plate and transfected with Lipofectamine 2000 according to the manufacturer protocols. After 24 h culture medium was substituted with DMEM only to induce cell starvation. After 24 h 1 × 106 cells were resuspended in 100 µl of FACS buffer (DMEM no red phenol, FBS 0.1%, Hepes 12 mM) added with P.I 1:1000 (stock solution 1 mg/ml) and Annexin V 1:100 (Roche, Cat no. 118286810001). Samples were incubated dark at room temperature for 20 min and then analyzed by flow cytometry.
Falconieri A., Minervini G., Bortolotto R., Piovesan D., Lopreiato R., Sartori G., Pennuto M, & Tosatto S.C. (2020). The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel–Lindau tumor suppressor. Scientific Reports, 10, 15850.
+ Open protocol
+ Expand
10

Annexin V and Propidium Iodide Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometric detection, 20,000 cells/well were seeded in 24-well microtiter plates and subjected to the appropriate treatment. Next, they were washed and trypsinized. Detached cells and trypsinized adherent cells were collected, pooled, and centrifuged. The cell pellet was resuspended in 50 μl HEPES buffer (10 mM Hepes/NaOH, pH 7.4, 140 mM NaCl, 5 mM CaCl2) and stained with propidium iodide (Sigma-Aldrich) and Annexin V (Roche, Mannheim, Germany). After a 10-minute incubation, samples were analyzed in a BD FACS Canto II (BD Biosciences, Heidelberg, Germany) using FACS Diva Software.
Kiprianova I., Remy J., Milosch N., Mohrenz I.V., Seifert V., Aigner A, & Kögel D. (2015). Sorafenib Sensitizes Glioma Cells to the BH3 Mimetic ABT-737 by Targeting MCL1 in a STAT3-Dependent Manner. Neoplasia (New York, N.Y.), 17(7), 564-573.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!