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Ms400 linear array transducer

Manufactured by Fujifilm
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The MS400 is a linear array transducer manufactured by Fujifilm. It is designed for use in medical imaging applications. The transducer utilizes an array of piezoelectric elements to generate and receive ultrasound signals, allowing for the creation of detailed images of internal structures.

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Lab products found in correlation

Vevo 2100 system, by Fujifilm (7 mentions) Vevo 2100, by Fujifilm (4 mentions) Vevo2100 lazr imaging system, by Fujifilm (2 mentions) Bp 98a, by Softron (1 mentions)

15 protocols using ms400 linear array transducer

1

Echocardiographic Monitoring of Cardiac Function

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Serial echocardiography was performed and interpreted according to standard protocols by a blinded investigator. Briefly, animals were anesthetized with isoflurane. Echocardiographic images were obtained with a Vevo 2100® system (Visualsonics, Inc., Toronto, Canada) equipped with a MS400 linear array transducer (30 MHz). The transducer was positioned in a stationary stand perpendicular to the mouse. A frame rate of > 200 frames per minute was maintained for all B‐ and M‐mode images. Measurements of ejection fraction, shortening fraction, end‐diastolic and ‐systolic volumes, interventricular and left posterior wall thickness, left ventricular internal dimension and left ventricular mass/body weight were obtained at weekly intervals from day 0 until day 42 in male 4‐week‐old mice injected with either PBS, LCWE or LCWE with IL‐1 receptor antagonist anakinra treatment.
Gorelik M., Lee Y., Abe M., Andrews T., Davis L., Patterson J., Chen S., Crother T.R., Aune G.J., Noval Rivas M, & Arditi M. (2019). IL‐1 receptor antagonist, anakinra, prevents myocardial dysfunction in a mouse model of Kawasaki disease vasculitis and myocarditis. Clinical and Experimental Immunology, 198(1), 101-110.
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2

Echocardiographic Evaluation of Mice

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Trans-thoracic echocardiography was performed on all mice by using a Vevo 2100 system with a MS400 linear array transducer (VisualSonics, ON, Canada) as previously reported (Zhang et al., 2018 (link)). Briefly, mice were anesthetized with 2% isoflurane and kept warm on a heated platform (37°C). The chest hairs were removed by using depilatory cream, and a layer of acoustic coupling gel was applied to the thorax. An average of 10 cardiac cycles of standard 2-D and m-mode short axis at mid-papillary muscle level were analyzed. Left ventricular ejection fraction and dimensions were calculated by using a modified Quinone method.
Bao Q., Zhang B., Suo Y., Liu C., Yang Q., Zhang K., Yuan M., Yuan M., Zhang Y, & Li G. (2020). Intermittent hypoxia mediated by TSP1 dependent on STAT3 induces cardiac fibroblast activation and cardiac fibrosis. eLife, 9, e49923.
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3

Echocardiographic Assessment of Cardiac Function

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TTE was performed on all mice at baseline (8 weeks post-TAC surgery) and 4 weeks post-randomization by using a Vevo 2100 System with an MS400 Linear Array Transducer (VisualSonics, ON, Canada) as described (Gao et al., 2011 (link)) After mice were weighed and anesthetized by inhalation of 1.5% isoflurane, they were placed on a heating mat to maintain normothermia (35°C). LAD, LV interventricular septum thickness diameter (IVSTd), LV posterior wall thickness diameter (LVPWd), LVDd, and LVEF were measured. LAAeV was obtained on the parasternal short-axis view (Figure 3A). After final echocardiography examination, mice were immediately sacrificed for heart tissues.
Suo Y., Yuan M., Li H., Zhang Y., Li Y., Fu H., Han F., Ma C., Wang Y., Bao Q, & Li G. (2019). Sacubitril/Valsartan Improves Left Atrial and Left Atrial Appendage Function in Patients With Atrial Fibrillation and in Pressure Overload-Induced Mice. Frontiers in Pharmacology, 10, 1285.
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4

Echocardiographic Assessment of Murine Cardiac Function

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Trans-thoracic echocardiography was performed on all mice using Vevo 2100 with a MS400 linear array transducer (VisualSonics), 18–38 MHz. Mice were anaesthetized with 2% isoflurane and kept warm on a heated platform (37 °C). The chest hairs were removed using depilatory cream and a layer of acoustic coupling gel was applied to the thorax. An average of 10 cardiac cycles of standard 2D and m-mode short axis at mid papillary muscle level according to a previously described method were obtained and stored for subsequent offline analysis41 (link). Left ventricular ejection fraction and dimensions were calculated using a modified Quinone method42 (link).
Schafer S., Viswanathan S., Widjaja A.A., Lim W.W., Moreno-Moral A., DeLaughter D.M., Ng B., Patone G., Chow K., Khin E., Tan J., Chothani S.P., Ye L., Rackham O.J., Ko N.S., Sahib N.E., Pua C.J., Zhen N.T., Xie C., Wang M., Maatz H., Lim S., Saar K., Blachut S., Petretto E., Schmidt S., Putoczki T., Guimarães-Camboa N., Wakimoto H., van Heesch S., Sigmundsson K., Lim S.L., Soon J.L., Chao V.T., Chua Y.L., Tan T.E., Evans S.M., Loh Y.J., Jamal M.H., Ong K.K., Chua K.C., Ong B.H., Chakaramakkil M.J., Seidman J.G., Seidman C.E., Hubner N., Sin K.Y, & Cook S.A. (2017). IL-11 is a crucial determinant of cardiovascular fibrosis. Nature, 552(7683), 110-115.
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5

In vivo Echocardiography of Aortic Dimensions

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In vivo trans-thoracic echocardiography was conducted using Vevo 2100 with a MS400 linear array transducer (VisualSonics), 18–38 MHz by a trained echocardiographer (NGZT) blinded to genotype and treatment groups. Aortic root and ascending aortic diameters were assessed from B- and M-mode of parasternal long-axis view, using inner edge-to-inner edge according to established guidelines66 (link). Peak aortic flow velocity was obtained by applying pulsed-wave Doppler across the aortic valve from the aortic arch at suprasternal view. All measurements were averaged over three cardiac cycles. Aortic dimensions were referenced to body weight per animal to account for differences in body mass.
Lim W.W., Corden B., Ng B., Vanezis K., D’Agostino G., Widjaja A.A., Song W.H., Xie C., Su L., Kwek X.Y., Tee N.G., Dong J., Ko N.S., Wang M., Pua C.J., Jamal M.H., Soh B., Viswanathan S., Schafer S, & Cook S.A. (2020). Interleukin-11 is important for vascular smooth muscle phenotypic switching and aortic inflammation, fibrosis and remodeling in mouse models. Scientific Reports, 10, 17853.
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6

Ultrasound Imaging of Aortic Expansion

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Ultrasound images of the abdominal aorta were obtained in mice anesthetized with isoflurane using a VisualSonics Vevo2100 LAZR imaging system equipped with an MS400 linear array transducer. The abdominal aortic diameter measurements were electrocardiogram-gated and measured in B-mode. External aortic diameters were measured during systole over various time points after aneurysm induction (baseline, day 7, day 14, day 21, and day 28) in both the axial and sagittal planes. The maximum aortic lumen diameter (aortic systolic diameter corresponding with cardiac systole) and the minimum aortic lumen diameter (aortic diastolic diameter corresponding with cardiac diastole) monitored by simultaneous electrocardiogram recordings were measured and used to calculate the aortic expansion index: ([systolic aortic diameter – diastolic aortic diameter]/systolic aortic diameter).15 (link)
Khoury M.K., Zhou T., Yang H., Prince S.R., Gupta K., Stranz A.R., Wang Q, & Liu B. (2020). GSK2593074A blocks progression of existing abdominal aortic dilation. JVS-Vascular Science, 1, 123-135.
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7

Comprehensive Echocardiographic Assessment of Mouse Cardiac Function

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Echocardiography was performed with the Vevo 2100 system (Visualsonics, Inc., Toronto, Canada) with a MS400 linear array transducer (30 MHz). The device’s transducer was positioned perpendicularly with a stand. Settings for the instrument are described in Table 1. Frame rate for both B-mode and M-mode was set to >200 frames per minute. B-mode long-axis parasternal images were recorded when optimal views of the aorta, papillary muscle, and endocardium were visible. M-mode short-axis images were recorded at the level of the papillary muscles and the LV was bisected to obtain the optimal M-Mode selection. At least 3 B-mode and M-mode images were captured for each mouse. All images were saved to a local computer and analyzed off line by a technician who was blinded to the animal’s age and group. Conventional echocardiographic measurements of the LV included EF, FS, end-diastolic dimension (EDD), end-systolic dimension (ESD), anterior and posterior wall thickness, and mass. For long-axis B-mode measurements, the endocardium was traced semi automatically beginning from the mitral valve and excluding the papillary muscle. EF and FS were calculated by software using standard computational methods.
Wang F., Iskra B., Kleinerman E., Alvarez-Florez C., Andrews T., Shaw A., Chandra J., Schadler K, & Aune G.J. (2018). Aerobic Exercise During Early Murine Doxorubicin Exposure Mitigates Cardiac Toxicity. Journal of pediatric hematology/oncology, 40(3), 208-215.
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8

Abdominal Aortic Diameter Measurement in Mice

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Ultrasound images of the abdominal aorta were obtained in mice anesthetized with isoflurane using a VisualSonics Vevo2100 LAZR imaging system equipped with an MS400 linear array transducer. The abdominal aortic diameter measurements were electrocardiogram-gated and measured in B-mode. External aortic diameters were measured during systole over various time points after aneurysm induction (baseline, day 7, day 14, day 21, and day 28) in both the axial and sagittal planes. The maximum aortic lumen diameter (aortic systolic diameter corresponding with cardiac systole) and the minimum aortic lumen diameter (aortic diastolic diameter corresponding with cardiac diastole) monitored by simultaneous electrocardiogram recordings were measured and used to calculate the aortic expansion index: ([systolic aortic diameter – diastolic aortic diameter]/systolic aortic diameter).15 (link)
Khoury M.K., Zhou T., Yang H., Prince S.R., Gupta K., Stranz A.R., Wang Q, & Liu B. (2020). GSK2593074A blocks progression of existing abdominal aortic dilation. JVS-Vascular Science, 1, 123-135.
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9

Echocardiographic Assessment of Sepsis

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Echocardiographic assessment was performed 24 h after the CLP or LPS procedure in all experimental groups, including the control group. Echocardiographic imaging was conducted using a VisualSonics Vevo 2100 system equipped with an MS400 linear array transducer on mice anesthetized with 1% isoflurane. Mice were imaged at baseline (sham operated) and 24 h post-CLP or LPS, following a previously described protocol [21 (link),22 (link)]. To assess cardiac output, the recordings of at least 10 independent cardiac cycles for each experiment were analyzed. Cardiac output, the volume of blood pumped by the heart per minute, was then calculated based on these echocardiographic measurements, taking into consideration the heart rate, stroke volume, and the dimensions of the left ventricle. This method provides a comprehensive evaluation of the heart’s capacity to meet the body’s circulatory needs under septic conditions.
Ullah K., Li Y., Lin Q., Pan K., Nguyen T., Aniruddhsingh S., Su Q., Sharp W, & Wu R. (2023). Comparative Analysis of Whole Transcriptome Profiles in Septic Cardiomyopathy: Insights from CLP- and LPS-Induced Mouse Models. Genes, 14(7), 1366.
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10

Detailed Echocardiographic Evaluation of Mice

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All the mice underwent transthoracic echocardiography utilizing a Vevo 2100 system with an MS400 linear array transducer (Visual Sonics, Toronto, ON, Canada) in a blinded manner by a specialist physician. Briefly, the mice were anesthetized with isoflurane, the chest hair was removed, and the mice were placed in a supine position on a constant temperature table at 37 °C, and a small animal ultrasound system was used to record transthoracic echocardiographic images. Two-dimensional and M-mode images of the long-axis and short-axis sections of the parasternal left ventricle of the mouse heart were collected, and the LVID, LVPW, and IVS were measured. The left ventricular ejection fractions (LVEF), left ventricular fractional shortening (LVFS), and left ventricular volume (LV Vol) were calculated according to international standards. All the ultrasound parameters were based on the average of 5 consecutive cardiac cycles.
Zhang Y., Liu S., Peng J., Cheng S., Zhang Q., Zhang N., Zhou Z., Zhang Y., Zhao Y, & Liu T. (2023). Biomimetic Nanozymes Suppressed Ferroptosis to Ameliorate Doxorubicin-Induced Cardiotoxicity via Synergetic Effect of Antioxidant Stress and GPX4 Restoration. Nutrients, 15(5), 1090.
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