A rabbit polyclonal antibody against the peptide corresponding to amino acids 339 to 359 (CDGLNDSSKQGYSQETKRLKRT) of human PTAR1 primary sequence was custom made by YenZyme antibodies, LLC, CA 94080. The specificity of the antibody was validated by silencing PTAR1 in primary fibroblasts NHFs and HEK293T cell lines (Supplementary Fig. 1A ). Antibodies were from Abcam (FNTA, FNTB), Abnova (PGGT1B), Bethyl (RabGGTB), Aviva Biosystems (RabGGTA), Sigma (anti-FLAG M2, anti-FLAG, β-actin), Santa Cruz Biotechnology (SKP1), and Covance (anti-HA). A polyclonal rabbit antibody to FBXL2 was generated using a peptide against FBXL2 as previously described27 (link). Human cDNA ORFs for PTAR1, FNTA, and RabGGTA were purchased from Origene. GFP-tagged cDNA of RAB1a, RAB7a, RAB11, RAB34, and RAB35 were obtained from Addgene. The variously tagged-genes and mutants were generated in pcDNA3 and pEGFPC1 vectors either by sub-cloning or site-directed mutagenesis methodologies, as described.
Rabggta
Manufactured by OriGene
RabGGTA is a laboratory equipment product designed for protein purification. It serves as a tool for affinity chromatography, a technique used to separate and purify specific proteins from complex mixtures. The core function of RabGGTA is to facilitate the isolation and extraction of proteins of interest.
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Lab products found in correlation
2 protocols using rabggta
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Validation of PTAR1 antibody specificity
2
Validation of PTAR1 antibody specificity
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A rabbit polyclonal antibody against the peptide corresponding to amino acids 339 to 359 (CDGLNDSSKQGYSQETKRLKRT) of human PTAR1 primary sequence was custom made by YenZyme antibodies, LLC, CA 94080. The specificity of the antibody was validated by silencing PTAR1 in primary fibroblasts NHFs and HEK293T cell lines (Supplementary Fig. 1A ). Antibodies were from Abcam (FNTA, FNTB), Abnova (PGGT1B), Bethyl (RabGGTB), Aviva Biosystems (RabGGTA), Sigma (anti-FLAG M2, anti-FLAG, β-actin), Santa Cruz Biotechnology (SKP1), and Covance (anti-HA). A polyclonal rabbit antibody to FBXL2 was generated using a peptide against FBXL2 as previously described27 (link). Human cDNA ORFs for PTAR1, FNTA, and RabGGTA were purchased from Origene. GFP-tagged cDNA of RAB1a, RAB7a, RAB11, RAB34, and RAB35 were obtained from Addgene. The variously tagged-genes and mutants were generated in pcDNA3 and pEGFPC1 vectors either by sub-cloning or site-directed mutagenesis methodologies, as described.
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