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Uv vis spectrometer

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The UV-Vis spectrometer is a laboratory instrument that measures the absorption of ultraviolet and visible light by a sample. It is used to quantify the concentration of a substance in a solution by comparing the absorption spectrum of the sample to a known standard.

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Lab products found in correlation

Tla100 rotor, by Beckman Coulter (2 mentions) Furfural, by Sinopharm (1 mentions) Phenol, by Sinopharm (1 mentions)

Spelling variants of this product

DU 800 UV-vis spectrometer (10 citations) DU 640 Uv-Vis Spectrometer (3 citations)

3 protocols using uv vis spectrometer

1

Measuring LHCP Aggregation Prevention by cpSRP43

Cited 1 time
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The ability of cpSRP43 to prevent LHCP aggregation was measured as described previously.32 (link),36 (link) Aggregates were removed via ultra-centrifugation in a TLA-100 rotor (Beckman Coulter) at 100,000 rpm for 30 min at 4 °C prior to the experiment. Light scattering experiments were performed by addition of 3 μL of 50 μM LHCP denatured 8 M urea to 150 μL buffer D (50 mM KHEPES, pH 7.5 and 200 mM NaCl) or 2.5 μM cpSRP43 in buffer D. Light scattering was monitored at 360 nm on a UV-Vis spectrometer (Beckman Coulter) over time until equilibrium was reached. The percentage of soluble LHCP (% soluble) at equilibrium was plotted for each single-cysteine cpSRP43 mutant.
Siegel A., McAvoy C.Z., Lam V., Liang F.C., Kroon G., Miaou E., Griffin P., Wright P.E, & Shan S.O. (2020). A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone. Journal of molecular biology, 432(24), 166708.
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2

Measuring LHCP Aggregation Prevention by cpSRP43

Cited 1 time
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The ability of cpSRP43 to prevent LHCP aggregation was measured as described previously.32 (link),36 (link) Aggregates were removed via ultra-centrifugation in a TLA-100 rotor (Beckman Coulter) at 100,000 rpm for 30 min at 4 °C prior to the experiment. Light scattering experiments were performed by addition of 3 μL of 50 μM LHCP denatured 8 M urea to 150 μL buffer D (50 mM KHEPES, pH 7.5 and 200 mM NaCl) or 2.5 μM cpSRP43 in buffer D. Light scattering was monitored at 360 nm on a UV-Vis spectrometer (Beckman Coulter) over time until equilibrium was reached. The percentage of soluble LHCP (% soluble) at equilibrium was plotted for each single-cysteine cpSRP43 mutant.
Siegel A., McAvoy C.Z., Lam V., Liang F.C., Kroon G., Miaou E., Griffin P., Wright P.E, & Shan S.O. (2020). A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone. Journal of molecular biology, 432(24), 166708.
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3

Adaptive Culture of Ethanol-Tolerant Yeast

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S. cerevisiae, angel thermal- and ethanol-tolerant alcohol active dry yeast (product number: 80000012, Angel Yeast Co. Ltd., Hubei, China), was used in this study. The acclimatization of this yeast strain was conducted by multiple rounds of the adaptive culture in inhibitors medium containing acetic acid, furfural, and phenol (Sinopharm Chemical Reagent Co.,Ltd, Shanghai, China). S. cerevisiae can tolerate 5.3 g/L acetic acid, 1.3 g/L furfural, and 0.5 g/L phenol [36 (link)]. For seed preparation, S. cerevisiae was cultivated in yeast extract peptone dextrose (YPD) medium (20 g/L glucose, 10 g/L yeast extract, and 20 g/L peptone) at 30°C and 150 rpm for 12 hours. The yeast cells were then inoculated to the secondary seed medium (20 g/L glucose, 10 g/L yeast extract, and 20 g/L peptone) and cultivated at 30°C and 150 rpm for 12 hours. Cell density was measured at 600 nm (1-cm light path) using a UV-vis spectrometer (Beckman Coulter Inc., California, United States). OD was corrected between 0.1 and 0.7 with the dilution factors as necessary. The initial OD for secondary seed culture was 0.05.
Liu Z.H., Qin L., Zhu J.Q., Li B.Z, & Yuan Y.J. (2014). Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature. Biotechnology for Biofuels, 7, 167.
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