Rosa26tdtomato

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

The Rosa26tdTomato is a transgenic mouse model that expresses the td-Tomato fluorescent protein under the control of the ubiquitous Rosa26 promoter. The td-Tomato protein is a red fluorescent protein that can be detected using standard fluorescence microscopy techniques.

Automatically generated - may contain errors

Lab products found in correlation

57 protocols using rosa26tdtomato

Lineage Tracing of Mural Cells and Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Figure S1 illustrates the breeding strategy for generation of the mice used for tracing of mural cells and simultaneous identification of fibroblasts. Tamoxifen‐inducible NG2CreER(NG2^iCre, Jackson #008538) mice were bred in a db/+ background (NG2^iCre/+; db/+), then were crossed with ROSA26^tdTomato (Jackson #007914) also in the db/+ background ROSA26^tdTomato; (db/+), to generate NG2^iCre/+; ROSA26^tdTomato; db/+ mice. These animals were then crossed with PDGFRα^EGFP; db/+ fibroblast reporter mice to enable the simultaneous identification of mural cell‐derived progeny, as well as fibroblasts in the diabetic background, as previously described by our group.⁴⁶ For lineage tracing studies, 10‐ to 12‐week‐old NG2^iCre/+; R26^tdTomato; PDGFRα^EGFP; db/db or NG2^iCre/+; R26^tdTomato; PDGFRα^EGFP; db/+ (or +/+) mice received intraperitoneal injections of Tamoxifen (Sigma‐T5648, CAS#10540–29‐1), at a dosage of 100 mg/kg, administered over 5 consecutive days once every 24 hours. Mice were euthanized for histology when they reached 6 months of age.

Alex L., Tuleta I., Hanna A, & Frangogiannis N.G. (2023). Diabetes Induces Cardiac Fibroblast Activation, Promoting a Matrix‐Preserving Nonmyofibroblast Phenotype, Without Stimulating Pericyte to Fibroblast Conversion. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(6), e027463.

+ Open protocol

+ Expand

Punch Wound Healing Tracing Experiments

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

For all wounds 4 mm of disposable biopsy punches (no. 33-34, Integra Miltex) were used. PD49 mice were shaved and their back skin punch wounded, as described (Ge et al., 2017 (link)), then sacrificed at different time points.
For lineage tracing experiments, Lgr5-CreER;Rosa26 tdTomato (Jackson Laboratory) and Aspm-CreER;Rosa26 tdTomato transgenic mice (Madisen et al., 2010 (link), Marinaro et al., 2011 (link)) were used. First, these mice were injected with 10 μg μL⁻¹ of TM (200 μg g⁻¹ body). Then, GSK J1 (no. 4593, Tocris) and JIB 04 (no. 4972, Tocris) dissolved vehicles were applied on the back skin for a week. The wounded tissues were harvested after 1, 3, and 5 weeks after the punch wound.

Kang S., Long K., Wang S., Sada A, & Tumbar T. (2019). Histone H3 K4/9/27 Trimethylation Levels Affect Wound Healing and Stem Cell Dynamics in Adult Skin. Stem Cell Reports, 14(1), 34-48.

+ Open protocol

+ Expand

Mouse Genetic Models for Immunological Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6J wild-type mice (000664), Nr4a1^−/− (006187), Ccr2^rfp/rfp (017586), Cx3cr1^gfp/gfp (005582), Csf1r^eGFP (018549), Csf1^op (000231), and B6.SJL-Ptprc^aPepc^b/BoyJ mice (002014) were obtained from The Jackson Laboratory. CD45.1.2 mice were generated by crossing C57BL/6J (CD45.2) mice to B6.SJL-Ptprc^aPepc^b/BoyJ (CD45.1) mice. Generation of Flt3^cre/wt;Rosa26^LSL-eYFP/wt mice has been described previously10 (link). Nr4a1^flox mice were a gift from Professor Pierre Chambon, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch-Graffenstaden, France. Nr4a1^fl/fl mice were crossed to Csf1r^cre/wt (Jackson, 021024) mice and the resulting Nr4a1^fl/fl;Csf1^cre/wt mice were analyzed with Nr4a1^wt/wt;Csf1^cre/wt littermates used as controls. Nr4a1^eGFP/cre (Jackson, 016617) mice were crossed to Rosa26^tdTomato (Jackson, 007905) mice and the resulting Nr4a1^eGFP/cre+;Rosa26^tdTomato+ mice were analyzed using cre-negative littermates as controls. All experiments followed guidelines of the La Jolla Institute for Allergy and Immunology Animal Care and Use Committee, and approval for the use of rodents was obtained from the La Jolla Institute for Allergy and Immunology according to criteria outlined in the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health. Mice were euthanized by CO₂ asphyxiation and cervical dislocation.

Tacke R., Hilgendorf I., Garner H., Waterborg C., Park K., Nowyhed H., Hanna R.N., Wu R., Swirski F.K., Geissmann F, & Hedrick C.C. (2015). The transcription factor NR4A1 is essential for the development of a novel macrophage subset in the thymus. Scientific Reports, 5, 10055.

+ Open protocol

+ Expand

Transgenic Mouse Lines for Somatosensory Research

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments were performed with approval from the Georgia Institute of Technology Animal Use and Care Committee. MrgprC11^CreERT2 BAC transgenic line, MrgprB4^PLAP knockin line (Liu et al., 2007 (link)), and Pirt^GCamp3 knockin line (Kim Y. S. et al., 2014 (link)) were generously provided by Dr. Xinzhong Dong at the Johns Hopkins University. MrgprC11^CreERT2 BAC transgenic mouse line was generated by the Gene Targeting & Transgenic Facility at Janelia Farm. MrgprA3^GFP-Cre BAC transgenic line was generated by our previous study (Han et al., 2013 (link)). MrgprD^CreERT2 (Stock No: 031286), ROSA26^tdTomato (Stock No: 007914), ROSA26^IAP (Stock No: 009253), and ROSA26^DREADD (Stock No: 026220) mouse lines were purchased from Jackson Laboratory. All the mice used had been backcrossed to C57BL/6 mice for at least ten generations. All the animals were housed in the vivarium with a 12-h light/dark cycle, and all the behavioral tests were performed from 9 a.m. to 1 p.m. in the light cycle. Mice were housed in groups, with a maximum of five per cage with food and water ad libitum.

Xing Y., Steele H.R., Hilley H.B., Zhu Y., Lawson K., Niehoff T, & Han L. (2020). Visualizing the itch-sensing skin arbors. The Journal of investigative dermatology, 141(5), 1308-1316.

+ Open protocol

+ Expand

Genetic fate mapping of PDGFRβ+ cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

PDGFRßCreER^t2 (i.e. B6-Cg-Gt(Pdgfrß-cre/ERT2)^6096Rha/J, JAX Stock #029684) and Rosa26tdTomato (i.e. B6-Cg-Gt(ROSA)26Sort^{tm(CAG-tdTomato)Hze}/J JAX Stock #007909) were purchased from Jackson Laboratories (Bar Harbor, ME, USA). Pdgfrb-BAC-eGFP reporter mice were developed by N. Heintz (The Rockefeller University) for the GENSAT project. UUO was performed as previously described using male and female mice.^{26 (link)} Animal experiment protocols were approved by the LANUV-NRW, Germany and by the UK Home Office Regulations. For Smart-Seq2, PDGFRbeGFP male mice were used born within 10 days of each other, and between 9 and 11 weeks old at the time of surgery. For inducible fate tracing PDGFRbCreER;tdTomato mice (8 weeks of age) received tamoxifen (10mg p.o.) 3 times via gavage followed by a washout period of 21 days and then subjected to UUO surgery or sham (as above) and sacrificed at 10 days after surgery. Mice were housed two to five animals per cage with a 12-h light–dark cycle (lights on from 0700 to 1900 h) at sustained temperature (20 °C±0.5°C) and humidity (~50%±10%) with ad libitum access to food and water.

Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.

+ Open protocol

+ Expand

Transgenic Mice for Muscle Stem Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mice strains were obtained from Jackson Laboratories, including C57BL/6J mice (Stock no. 000664, adult mice at 8 weeks and aged mice at 18 months), mdx mice (C57BL/10ScSn-Dmdmdx/J, Stock No: 001801), Rosa26-tdTomato (Stock no. 7909), Pax7-cre/ERT2 (Stock no. 017763), and Rosa26-EYFP (Stock no. 006148). Pax7-cre/ERT2 and Rosa26-EYFP were crossed to produce Pax7-CreER:Rosa26-EYFP offspring. The genotypes of all transgenic mice were confirmed with genotyping analyses according to the manufacturer’s instructions. All mice were bred and maintained in specific pathogen-free conditions. All animal work was conducted under protocols approved by the UC Berkeley or UCLA Animal Research Committee.

Fang J., Sia J., Soto J., Wang P., Li L.K., Hsueh Y.Y., Sun R., Francis Faull K., Tidball J.G, & Li S. (2021). Skeletal-muscle regeneration via the chemical induction and expansion of myogenic stem cells in situ or in vitro. Nature biomedical engineering, 5(8), 864-879.

+ Open protocol

+ Expand

Conditional Dmrt1 Deletion in Mouse Gonads

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dmrt1^flox/flox mice were bred to either Oct4-CreERT2 [28 (link)] (gift of Dr. Yoav Segal) or Ngn3-Cre [29 (link)]; (gift of Dr. Shosei Yoshida) and to Id4-Gfp [12 (link)] and Rosa26-tdTomato transgenic mice (Jackson Laboratories Cat #: 0007914). For experiments involving conditional deletion of Dmrt1, Dmrt1^flox/+ mice were used as controls and Dmrt1^flox/flox mice were used as experimentals. Both controls and experimental animals carried the Oct4-cre transgene and were treated with tamoxifen. In busulfan depletion experiments, controls and experimental animals carried the Ngn3-cre transgene and were either Dmrt1^flox/+ (control) or Dmrt1^flox/flox (experimental). Experimental protocols were approved by the University of Minnesota Institutional Animal Care and Use Committee.

Zhang T., Oatley J., Bardwell V.J, & Zarkower D. (2016). DMRT1 Is Required for Mouse Spermatogonial Stem Cell Maintenance and Replenishment. PLoS Genetics, 12(9), e1006293.

+ Open protocol

+ Expand

Generation of Zeb2 Conditional Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zeb2-floxed mice and the genotyping protocol were described previously (40 (link)). Foxd1-Cre⁺ (stock 012463) and Rosa26tdTomato (stock 007909) mice were purchased from the Jackson Laboratory and genotyped according to the protocol as provided by the Jackson Laboratory. Eight-week-old Zeb2^fl/+;Foxd1-Cre⁺ mice were bred with Zeb2^fl/fl mice to generate Zeb2^fl/fl;Foxd1-Cre⁺ homozygous cKO mice. All mice had free access to drinking water and a standard rodent diet ad libitum. Animals of both sexes in a mixed C57BL/6 × 129 genetic background were used in this study. Littermate wild-type mice were used as controls.

Kumar S., Fan X., Rasouly H.M., Sharma R., Salant D.J, & Lu W. (2023). ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis. JCI Insight, 8(1), e158418.

+ Open protocol

+ Expand

Lineage Tracing and Cell Ablation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mouse experiments were performed according to the animal experimental guidelines issued by the Animal Care and Use Committee at Harvard University. Gli1-nLacZ (JAX #008211) Gli1CreER^t2 (JAX #007913), Rosa26tdTomato (JAX #007909) iDTR mice (JAX # 007900), Ptprc^a-Pepc^b mice (JAX #002014) were purchased from Jackson Laboratories (Bar Harbor, ME). For lineage tracing studies 6–7 week old mice received 3 × 0.1mg/kg bodyweight tamoxifen in corn oil / 3% ethanol (Sigma) via intraperitoneal injection 10days before surgery or disease induction unless otherwise stated. All models of organ injury and cell-specific ablation experiments are described in the Supplementary Experimental Procedures.

Kramann R., Schneider R.K., DiRocco D.P., Machado F., Fleig S., Bondzie P.A., Henderson J.M., Ebert B.L, & Humphreys B.D. (2014). Perivascular Gli1+ progenitors are key contributors to injury-induced organ fibrosis. Cell stem cell, 16(1), 51-66.

+ Open protocol

+ Expand

Mouse Strain Fate Mapping and Hair Cell Ablation

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following mouse strains were used: Atoh1-CreERTM, a gift from S. Baker, St. Jude’s Hospital, Memphis, TN (31 (link)), Rosa26-tdTomato (The Jackson Laboratory, stock 007908) (32 (link)), Pou4f3-DTR (The Jackson Laboratory, stock 028673) (7 (link)), and Plp1-CreERT (The Jackson Laboratory, stock 5975) (63 (link)). Mice of both sexes were used. To induce Cre-recombinase activity for fate mapping, Atoh1^CreERTM/+; Rosa26^tdTomato/+ mice were injected with tamoxifen (intraperitoneally, 0.075 mg/g dissolved in corn oil; Sigma) at P1. To selectively ablate hair cells, mice carrying the Pou4f3-DTR allele were treated with DT at P1 (intramuscularly, 4 ng/g) immediately prior to tamoxifen injection. For Pou4f3^DTR/+; Plp1^CreERT/+; Rosa26R^tdTomato/+, DT was administered at P1 (intramuscularly, 4 ng/g) to ablate hair cells followed by tamoxifen injection at P8 (intraperitoneally, 0.075 mg/g dissolved in corn oil) for Cre activation (11 (link)). Mice were housed in the Stanford University Veterinary Service Center that is fully accredited by the Association for Accreditation and Assessment of Laboratory Animal Care. Food and water were available ad libitum. The mouse room was maintained on a 12-h light/12-h dark cycle in a quiet room. All protocols were approved by the Animal Care and Use Committee of the Stanford University School of Medicine (18606).

Kim G.S., Wang T., Sayyid Z.N., Fuhriman J., Jones S.M, & Cheng A.G. (2022). Repair of surviving hair cells in the damaged mouse utricle. Proceedings of the National Academy of Sciences of the United States of America, 119(15), e2116973119.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!