HER2: Tissue sections were baked at 60°C for 60 min and then placed in ventana BenchMark Ultra for staining. The procedure is as follows: EZ prep dewaxing, ULTRA CC1 antigen repair for 36 min, HER2 antibody incubation at 37°C for 32 min, ultraView Universal DAB Detection Kit detection system color rendering, hematoxylin II nuclear restaining. Bluing reagent returns blue. For other antibodies: Tissue sections were baked at 60°C for 60 min and then placed in Leica Bond III for staining. The procedure is as follows: BOND Dewax, BOND Epitope Retrieval 2 Antigen repair at 97°C for 20 min, endogenous peroxidase blocking for 10 min, primary antibody incubation for 15 min, BOND Polymer Refine Detection for secondary antibody incubation for 16 min. DAB color development for 10 min, hematoxylin redyeing for 2 min after returning to blue. Sy: Clone DAK‐SYNAP, ready‐to‐use antibody, supplier Dako, staining platform Leica Bond III. CgA: Clone 317F1D8, ready‐to‐use antibody, supplier Suzhou Baidao, staining platform Leica Bond III. CD56: Clone MX039, dilution ratio 1:600, supplier Fuzhou Maixin, dyeing platform Leica Bond III.
Bond 3
Manufactured by Leica
Sourced in Germany, United States, United Kingdom, Australia, Italy, Switzerland, Japan, Denmark, Israel, Azerbaijan
The Bond III is a laboratory instrument designed for biomolecular interaction analysis. It is capable of measuring and analyzing the binding interactions between various molecules, such as proteins, peptides, small molecules, and nucleic acids. The Bond III provides real-time kinetic data and quantitative information about the strength and specifics of these interactions.
Automatically generated - may contain errors
Lab products found in correlation
Bond polymer refine detection kit, by Leica (42 mentions)
Bond polymer refine detection, by Leica (15 mentions)
Benchmark ultra, by Roche (14 mentions)
Bond polymer refine detection system, by Leica (11 mentions)
Ar9640, by Leica (6 mentions)
Refine detection kit, by Leica (5 mentions)
Anti brdu antibody b2531, by Merck Group (4 mentions)
Anti ki 67 antibody vp rm04, by Vector Laboratories (4 mentions)
Ventana benchmark ultra, by Roche (4 mentions)
Mib 1, by Agilent Technologies (4 mentions)
Ds9800, by Leica (4 mentions)
Ventana benchmark, by Roche (4 mentions)
E1l3n, by Cell Signaling Technology (4 mentions)
Bond max, by Leica (4 mentions)
Clone e1l3n, by Cell Signaling Technology (4 mentions)
Benchmark ultra, by Leica (3 mentions)
Biosystems refine detection kit, by Leica (3 mentions)
M7103, by Agilent Technologies (3 mentions)
Epitope retrieval solution 2, by Leica (3 mentions)
Bond epitope retrieval solution 2, by Leica (3 mentions)
387 protocols using bond 3
1
Immunohistochemical Staining of HER2, Synaptophysin, and CD56
2
Immunohistochemical Analysis of Liver and Intestine
3
PD-L1 Immunohistochemistry Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serial sections were cut and stained using HES (hematoxylin, eosin, saffron) for histopathological diagnosis. PD-L1 staining was performed on Benchmark Ultra (Ventana Medical Systems) and Bond-III (Leica Biosystems) stainers using similar protocols outlined as follows. Benchmark Ultra (according to assay instructions): antigen retrieval 4 min at pH 9, incubation with SP142 for 8 min, and counterstaining with hematein for 8 min; Leica Bond-III: antigen retrieval 20 min at pH 9, incubation with SP142 for 15 min, and counterstaining with hematein for 10 min.
Diagnosis of BC and PD-L1 assessment was performed by a senior urogenital pathologist (EC) with training and experience in SP142 PD-L1 assessment standards [4 (link)]. Slides from the Benchmark Ultra were scored on a case-by-case basis for diagnostic purposes between 2019 and 2020. Cases were then collectively stained on the Bond-III and scored in a randomized manner, blinded to the results of the Benchmark Ultra. Although not part of the approved SP142 assay, tumor cell (TC) scores were also assessed for the purpose of this study. PD-L1 IC and TC scores were scored in categories of 0–5%, 5–10%, 10–30%, and > 30%.
Diagnosis of BC and PD-L1 assessment was performed by a senior urogenital pathologist (EC) with training and experience in SP142 PD-L1 assessment standards [4 (link)]. Slides from the Benchmark Ultra were scored on a case-by-case basis for diagnostic purposes between 2019 and 2020. Cases were then collectively stained on the Bond-III and scored in a randomized manner, blinded to the results of the Benchmark Ultra. Although not part of the approved SP142 assay, tumor cell (TC) scores were also assessed for the purpose of this study. PD-L1 IC and TC scores were scored in categories of 0–5%, 5–10%, 10–30%, and > 30%.
4
Immunohistochemical Analysis of Liver and Intestine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Upon sacrifice, liver and small intestine were collected. Liver was washed in PBS briefly and small intestine was flushed and washed with PBS. Tissues were fixed in 4% formaldehyde for 48hrs and transferred to 70% ethanol before paraffin-embedded and sectioned. Anti-BrdU antibody (B2531) was from Sigma. The antibody was run on an automated platform, Leica BOND-III. The retrieval step used Leica’s ER1 solution for 30min, and the primary antibody was incubated at 1:2000 dilution for 30min. The Bond Polymer Refine Detection kit was used which includes the secondary and DAB chromogen detection. The Ki-67 was also run on the BOND-III using the same detection kit. Anti-Ki-67 antibody (VP-RM04) was from Vector and was incubated at 1:250 dilution for 30min. The retrieval solution used on the BOND-III was Leica's ER2 antigen retrieval solution and incubated for 20min.
5
Automated Immunohistochemistry for COVID-19 Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For IHC, an automated IHC stainer BOND-III (Leica Biosystems) was used. Briefly, 4 μm sections of paraffin-embedded kidneys were submitted for appropriate antigen retrieval. Then, sections were incubated with the following antibodies: rabbit monoclonal anti-ACE2 antibody (Abcam, ab108252, 1:100), rabbit monoclonal anti–cleaved caspase-3 antibody (Cell Signaling Technology, 9664, 1:200), rabbit polyclonal anti–recombinant nucleoprotein from SARS-CoV antibody (a gift from Nicolas Escriou, Institut Pasteur, Paris, France), and rabbit polyclonal SARS-CoV-2 anti-nucleoprotein (Novusbio, NB100-56576, 1:500). ACE2 expression was evaluated by image quantification using the Integrated Density program of ImageJ software (NIH) on whole-kidney sections (×200). This measurement integrates the product of area and the mean intensity value above a specific threshold. This estimates the amount of the strength of the expression of a specific epitope.
Immunofluorescence was performed on frozen kidney biopsies using antibodies targeting the heavy chains of immunoglobulins (IgA, IgG, IgM), kappa and lambda light chains, complement (C3, C1q), and fibrinogen using the automated stainer BOND-III (Leica Biosystems). Immunofluorescence staining was performed on 26 of 32 biopsies.
Immunofluorescence was performed on frozen kidney biopsies using antibodies targeting the heavy chains of immunoglobulins (IgA, IgG, IgM), kappa and lambda light chains, complement (C3, C1q), and fibrinogen using the automated stainer BOND-III (Leica Biosystems). Immunofluorescence staining was performed on 26 of 32 biopsies.
6
Immunohistochemical Staining Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemical staining was performed as previously described (22 (link), 23 (link)). Briefly, 8 μm-thick sections were cut, dehydrated, and processed on a Bond III (Leica Biosystems Wetzlar, Germany). The ready-to-use (RTU) primary antibodies were from Novocastra (Leica Biosystems, Wetzlar, Germany), optimized for use on Bond III. The staining was performed with the following antibodies:
7
Immunohistochemical Analysis of EBV-Related Lymphomas
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue samples were fixed in 10% formalin and embedded in paraffin (FFPE), followed by staining of 3 μm-thick sections with hematoxylin and eosin. The immunohistochemistry for 12 EBVMCU cases was performed by an autoimmunostainer using BenchMark ULTRA (Ventana, Oro Valley, AZ, USA) and Leica Bond-III (Leica Biosystems, Wetzlar, Germany). Monoclonal antibodies were as follows; anti-CD3/LN10, CD20/L26, CD10/56C6, BCL6/LN22, EBNA2/PE2 (Novocastra Laboratories, Newcastle, UK), CD30/Ber-H2, MUM-1/MUM1p, LMP1/C.S1-4 (DAKO, Santa Fe, CA, USA), CD15/MMA (Becton Dickinson and Company, NJ, USA), PD-L1/E1J2J (Cell Signaling Technology, MA, USA), PD-L1/SP142 (Roche, Basel, CHE). EBV infection was evaluated by in situ hybridizations for EBV-encoded small RNA (EBER) oligonucleotide probe (PB0589, Leica Biosystems) on FFPE sections using Leica Bond-III.
8
NSCLC Tumor Tissue IHC Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 130 deidentified, archival formalin-fixed, paraffin-embedded (FFPE) tumor tissues (1 tissue sample/patient) from NSCLC patients were used in this study. All the FFPE blocks were processed, reviewed, and stored in a College of American Pathologists (CAP) accredited and Clinical Laboratory Improvement Amendments (CLIA) certified laboratory (Tempus Labs Chicago, IL, USA).
Each FFPE block was cut into 4 μm-thick serial sections for H and E and IHC stains. H and E staining was performed on the Leica Autostainer XL staining platform. The sections were stained with H and E and anti-PD-L1 (clone 22c3, pharmDx Kit Dako) using an automated staining system (BOND-III: Leica Microsystems). IHC slides were stained with anti-PD-L1 22C3 monoclonal mouse primary antibody using the Bond Polymer Refine detection system on a Leica Microsystems BOND-III with positive and negative cell line run controls.
Each FFPE block was cut into 4 μm-thick serial sections for H and E and IHC stains. H and E staining was performed on the Leica Autostainer XL staining platform. The sections were stained with H and E and anti-PD-L1 (clone 22c3, pharmDx Kit Dako) using an automated staining system (BOND-III: Leica Microsystems). IHC slides were stained with anti-PD-L1 22C3 monoclonal mouse primary antibody using the Bond Polymer Refine detection system on a Leica Microsystems BOND-III with positive and negative cell line run controls.
9
Comprehensive Histological Analysis of Murine Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Murine brain tissue was processed on a Leica Peloris II histological platform (Leica, Wetzlar, Germany) and H&E stained using a Leica Autostainer XL platform (Leica). PPARα, Ki67 and EGFR IHC was carried out using a Leica Bond III automated immunostainer (Leica). IDH1, ATRX and GFAP IHC were carried out using a Ventana BenchMark ULTRA platform (Roche, Basel, Switzerland). Antigen retrieval techniques and antibody concentrations are detailed in supplementary material, Supplementary materials and methods and Table S4 . EGFP immunofluorescence was carried out as described previously 38 .
10
Immunohistochemical Profiling of Lymphoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematoxylin and Eosin (H&E) stained FFPE tissue sections were reviewed to assess cyto-architectural features. Immunohistochemistry and in-situ hybridization was performed with the following panel of antibodies and probes; CD20 (clone MJ1); CD10 (clone 56C6); CD5 (clone 4C7); BCL6 (clone LN22); BCL2 (clone D5); CD21 (clone PA0171); kappa (clone ISH-5748A), lambda (clone ISH-5770A), all from Leica, IL, USA; CD79a (clone AP18); Cyclin D1 (clone SP4-R); CD138 (clone B-A38); CD21 (clone PA0171); IgG (clone 1210208A); IgG4 (clone 1123107A); Ki-67 (clone 30-9), all from Ventana, AZ, USA; and MUM-1 (clone MUM1p) from DAKO, CA, USA. Staining was performed with automated stainers (Ventana Benchmark Ultra and Leica Bond III) and visualized with the UltraView Universal and Bond polymer DAB detection kits according to the manufacturer's protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!