Two hundred and fifty grams of whole by-product mussels were ground using a tabletop cutter blender (Robot Coupe R2 tabletop cutter mixer, Vincennes, France) with 500 mL water for 1 min. The slurry obtained was used for hydrolysis and endogenous enzymes were heat-deactivated for 10 min at 80 • C in a water bath prior to hydrolysis. Hydrolysis was carried out at 130 rpm, 35 • C, pH 7 with Protamex ® added to the mussel by-product at the ratio of 1:50 (w:v). Hydrolysis was carried out for 1.5 h. Then, 0.1 M NaOH was added to adjust the pH of the hydrolysates to 7. Protamex ® was heat-deactivated at 95 • C for 10 min and hydrolysates allowed to cool to room temperature. Clean mussel shells were separated from the mixture, the hydrolysate slurries were poured into trays, frozen and then freeze-dried using a Labconco freeze drier (Labconco corporation, Kansas City, Missouri, USA) for 48 h. The freeze-dried hydrolysates were weighed to calculate yield and were subsequently analysed for protein, lipid, ash and fatty acid methyl ester (FAME) content.
Freeze drier
Manufactured by Labconco
Sourced in United States
The Freeze Dryer is a laboratory equipment designed for the process of freeze-drying, which removes water from a frozen sample by sublimation. It provides a controlled environment for the preservation of sensitive materials, such as biological samples, pharmaceuticals, and food products, by preserving their original structure and properties.
Automatically generated - may contain errors
Lab products found in correlation
Spherisorb 5 m ods2 4.6 50 mm analytical column, by Waters Corporation (2 mentions)
Enzymatic kits, by EKF Diagnostics (1 mentions)
Icp ms, by Agilent Technologies (1 mentions)
Freezone 2, by Labconco (1 mentions)
Ch 9230, by Büchi (1 mentions)
Filter paper, by Cytiva (1 mentions)
Trumac n, by Leco (1 mentions)
Nicolet is10, by Thermo Fisher Scientific (1 mentions)
Mini spray dryer b 290, by Büchi (1 mentions)
Ht7820, by Hitachi (1 mentions)
Tm3000, by Hitachi (1 mentions)
Vibra cell sonicator, by Sonics (1 mentions)
Rotary evaporator, by Büchi (1 mentions)
Rotary evaporator, by Eyela (1 mentions)
Supra 35, by Zeiss (1 mentions)
Desk 2, by Denton (1 mentions)
Methanol, by Avantor (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Formic acid, by Avantor (1 mentions)
α amylase, by Merck Group (1 mentions)
31 protocols using freeze drier
1
Producing Mussel Protein Hydrolysates
2
Roselle Powder Preparation Protocol
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The RECGs were placed in Petri dishes (13.76 ± 0.07 cm in diameter), covered with aluminum foil and frozen for a minimum of 72 hours in a CHTC-16E horizontal freezer (Torrey, Mexico) at -26 ± 0.5º C. The frozen RECG were lyophilized in a LabConco freeze drier (LabConco Corp. Kansas, City, USA) at 20°C and 0.005 to 0.01 mmHg of vacuum for 72 hours. The lyophilized RECG (Roselle powders = RP) were weighed, pulverized, placed in amber pharmaceutical jars, sealed with plastic wrap and capped. RP were stored in a desiccator with silica at room temperature (22 ± 2º C).
3
Evaluation of Magnesium Metabolism in Rats
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The rats in above 4 groups were hosted in individual metabolism cages for 24 h for separate collection of feces and urine weekly, i.e. at week 0, 1, and 2. Blood of these rats was also collected weekly for isolation of serum via centrifuge at 3000 g for 10 minutes. The feces, serum and acid treated urine were stored at −80 °C before analysis. These rats were sacrificed to harvest liver, kidney and heart at week 3 and 5 after oral administration. Feces, liver and kidney were kept in freeze drier (Labconco, Kansas, USA) for recording their dry weight. Then, the concentrated nitric acid was used to digest the samples for determining Mg ion concentration with the inductively coupled plasma mass spectrometer (ICP-MS, Agilent Technologies, Tokyo, Japan). In terms of serum, besides the measurement of Mg concentrations with ICP-MS, other biochemical indices, including creatinine, urea nitrogen, phosphorus, alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and tumor necrosis factor alpha (TNF-α) were also determined with enzymatic kits (Stanbio, Texas, USA).
4
Collagen-nanohydroxyapatite Scaffold Fabrication
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For batch A, Achilles tendon bovine collagen type I was dissolved using a diluted solution of HCl (10 mM) and kept at 4 °C. For batch B, the 12% Viscolma collagen suspension was used. In order to remove all of the lumps from the solutions, they were homogenized at 20,000 rpm (Ultra Turrax T25, IKA) at 4 °C for about 1 h and 30 min. In order to produce the samples, the collagen solution was mixed using a peristaltic pump, first with a HCl and nanohydroxyapatite solution, and after homogenization with a HCl, EDC (40 mM) and NHS (20 mM) solution. The collagen and nanoHA were in 50:50 % w/w proportions. The resultant solution filled two sizes of glass molds (5 and 10 cm of diameter) and was kept in the freezer for 24 h. Finally, the samples were dried using a freeze-drier (Labconco) for 24 h (−80 °C). Some samples were submitted to sterilization through e-beam irradiation (15 kGy). Eight different materials were studied, before (A and B) and after (A’ and B’) their e-beam irradiation, with 5 and 10 cm diameters.
5
Physalis peruviana L. Bioactive Extracts
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The extracts analyzed in this study were obtained from the mature berries and leaves of Physalis peruviana L. purchased of different local retail shops from the city of Guaranda (Ecuador). The berries and healthy leaves were washed and triturated, in parallel also were lyophilized in a Freeze drier (Labconco Free Zone 2.5, USA), for their later processed. Fifteen grams of uvilla (berries and leaves) were put to maceration in 100 ml of 95% ethanol and 100 ml of distiller water at room temperatura for 6 d. According to the method established by Çakiret al. [6] for ethyl extract and Areiza et al. [16] for aqueous extract with so memodifications. In table 1, the treatments performed in this research are detailed where a DBCA was applied with factorial arrangement AxB: A (the type of Extract) and B (Parts of the vegetable).
6
Preparation of Jamaican Herbal Infusion
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The following method of preparation was previously developed and refined in our laboratory [10 ,16 (link)]. Collected leaf and stem plant material was bench-dried in our laboratory and then finely crushed using a coffee grinder. The ground plant material was prepared as an infusion following traditional Jamaican practices [17 (link),19 (link)] using 100 mL of boiled deionized water per 1 g of dried plant material and infused for fifteen to twenty minutes. The resulting liquor was suction filtered through type 1 Whatman filter paper and centrifuged (MSE Micro Centaur, Sanyo, Osaka, Japan) at 13,000 g for five minutes to remove suspended solids. The samples were frozen at −20 °C in round bottom flasks and lyophilised using a freeze drier (Labconco, Kansas City, MO, USA). The resulting solids were placed in vials and kept at −20 °C until required, and not subjected to more than two freeze-thaw cycles.
7
Freeze-Drying and Milling of Fruits
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Fresh fruits were delivered to the laboratory, washed, and dried. Seeds from fruits were removed. Fruits were freeze-dried in a LabconCo freeze-drier (Kansas City, MI, USA) at −40 °C and under a pressure of 0.100 mbar. Freeze-dried fruit material was ground in an A-11 lab Mill and further stored at −80 °C before further chemical composition analyses.
8
Freeze-Drying of Feijoa Fruits
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Fresh feijoa fruits of Apollo, Unique, Opal Star and Wiki Tu cultivars, at the commercial ripening stage, were kindly provided by local orchards in the Northern island of New Zealand. Feijoas were washed carefully to remove external attachments upon arrival at the Food Science laboratories at the University of Auckland. Feijoa flesh and peel were separated using a manual peeler, and the flesh and whole fruit samples were cut into 1 cm-thick slices. The prepared feijoa samples were kept at −20 °C for 12 h followed by storage at −80 °C for 4 h, and then transferred to a freeze drier (Labconco, USA). After 72 h of freeze drying, the freeze-dried feijoa slices were further ground into a fine powder using a coffee grinder (Breville BCG200, Sydney, Australia) and the obtained samples were stored at −80 °C until required.
9
Bioremediation Using C. necator GX_5
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The strain of C. necator GX_5 was incubated in a Luri–Bertani broth medium at a pH of 7.0 ± 0.2 on a rotary shaker at 180 rpm and 28 °C until the logarithmic growth period, reaching an OD600 of approximately 1.0. For the living biosorbents, the biomass was collected by centrifuging at 10,000 rpm for 10 min. It was then washed three times with sterile distilled water, pre-cooled at − 80 ℃ and lyophilised 24 h with a Labconco freeze drier [24 (link)]. Thereafter, the dried pellet was ground into powder before use. For the non-living cells, the living bacterial suspension was inactivated using a high-pressure steam sterilization [25 (link)]. Dead biosorbent was prepared similarly to the abovementioned methods for the live ones. Meanwhile, the biosorbent dosages (concentrations) were calculated by grams per litre.
10
Aniracetam-HP-β-CD Inclusion Complexes
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Solid inclusion complexes of HP-β-CD and aniracetam were prepared by physical mixture for 30 min and freeze-drying methods in molar ratios of 1:1. To prepare the samples for lyophilization, aniracetam was added to HP-β-CD 50% w/v solution in a molar ratio of 1:1. The mixture was then shaken for 24 h at 200 RPM in an orbital mixture incubator (Ratek, Newcastle, Australia). Upon completion, the solution containing the aniracetam-HP-β-CD inclusion complexes was transferred into plastic vials in volumes of 2 mL. Sucrose was then added to each 2 mL sample, the amount being 20% of the total aniracetam/cyclodextrin weight. Samples were then frozen at −83 °C for >5 h. The frozen samples were then dried using a freeze drier (Labconco, Fort Scott, KS, USA) for 48 h.
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