2400 series 2 chns o

Manufactured by PerkinElmer

Sourced in United States

The 2400 Series II CHNS/O is a high-performance elemental analyzer designed for the rapid and accurate determination of carbon, hydrogen, nitrogen, sulfur, and oxygen in a variety of organic and inorganic samples. It utilizes advanced combustion and gas chromatography technology to provide precise analytical results.

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Lab products found in correlation

Tlc silica gel 60 f254 plate, by Merck Group (2 mentions) Miniflex 600, by Rigaku (1 mentions) Ft ir 4600, by Jasco (1 mentions) Av 500 nmr spectrometer, by Bruker (1 mentions) Rf 5301pc spectrofluorophotometer, by Shimadzu (1 mentions) Gcms qp2010 ultra, by Shimadzu (1 mentions) Multisizer 4 coulter counter, by Beckman Coulter (1 mentions) Qp2020 nx, by Shimadzu (1 mentions) 6400 automatic isoperibol calorimeter, by Parr (1 mentions) Agilent 720, by Agilent Technologies (1 mentions) Toc l analyzer, by Shimadzu (1 mentions) Ics 1100, by Thermo Fisher Scientific (1 mentions) Aqf 2100h, by Mitsubishi (1 mentions) Aa 7000, by Shimadzu (1 mentions) Cpc 411, by Elmetron (1 mentions) Ad 6 autobalance, by PerkinElmer (1 mentions)

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2400 Series II (45 citations) CHN 2400 (14 citations) 2400 II CHNS (7 citations)

20 protocols using 2400 series 2 chns o

Synthesis of Pyrano[2,3-f]chromene Derivative

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All chemicals were purchased from commercial suppliers and used as delivered. ¹H NMR, ¹³C NMR and ¹⁹F NMR spectra were recorded at 400 (or 600), 100 (or 150) and 376 (or 565) MHz, respectively. Chemical shifts are reported in parts per million (ppm) and coupling constants in Hertz (Hz). Residual solvent peaks in DMSO-d₆ (δ = 2.50 ppm) [22 (link)] and CDCl₃ (δ = 7.26 ppm) served as internal standards for recording. Microanalyses were performed on PerkinElmer Series II CHNS/O 2400 elemental analyzer. Melting points were determined using a Stuart SMP 3 apparatus. Thin-layer chromatography (TLC) was performed using Merck silica gel 60 F₂₅₄ TLC plates.
Images of ¹H and ¹³C NMR spectra are provided in the Supplementary Materials.
5-Hydroxy-2,2-dimethyl-10-propyl-2H,8H-pyrano[2,3-f]chromen-8-one 3 was prepared from 5,7-dihydroxy-4-propylcoumarin according to the published procedure [21 (link)].

Khalymbadzha I.A., Fatykhov R.F., Butorin I.I., Sharapov A.D., Potapova A.P., Muthipeedika N.J., Zyryanov G.V., Melekhin V.V., Tokhtueva M.D., Deev S.L., Kukhanova M.K., Mochulskaya N.N, & Tsurkan M.V. (2024). Bioinspired Pyrano[2,3-f]chromen-8-ones: Ring C-Opened Analogues of Calanolide A: Synthesis and Anti-HIV-1 Evaluation. Biomimetics, 9(1), 44.

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Synthesis and Characterization of Novel Compounds

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All the chemicals were purchased from commercial suppliers and used as delivered. The ¹H NMR and ¹³C NMR spectra were recorded at 400 and 100 MHz, respectively (the NMR spectra of all the new compounds are available in the Supplementary Materials). The chemical shifts were reported in parts per million (ppm) and the coupling constants in Hertz (Hz). Tetramethylsilane (TMS) (δ = 0.00 ppm) or the residual solvent peak in DMSO-d₆ (δ = 2.50 ppm) and CDCl₃ (δ = 7.26 ppm) served as the internal standard for recording [34 (link)]. The molecular weights of unknown compounds were checked by the LC-MS 6200 series supplied by Agilent Technology. The microanalyses were performed on a PerkinElmer Series II CHNS/O 2400 elemental analyzer. The melting points were determined using a Stuart SMP 3 apparatus. Thin-layer chromatography (TLC) was performed using Merck silica gel 60 F₂₅₄ TLC plates.

Babu A., Sunil K., Sajith A.M., Reddy E.K., Santra S., Zyryanov G.V., Venkatesh T., Bhadrachari S, & Nibin Joy M. (2024). NMI-SO2Cl2-Mediated Amide Bond Formation: Facile Synthesis of Some Dihydrotriazolopyrimidine Amide Derivatives as Potential Anti-Inflammatory and Anti-Tubercular Agents. Pharmaceuticals, 17(5), 548.

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Synthesis and Characterization of Compound 3

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Compound 3 and (anilinium) 3 [Fe III (dto) 3 ](H 2 O) were prepared by a reported procedure. 18 Compound n PrPh 3 P bromide was purchased from Sigma-Aldrich. Infrared (IR) spectra were recorded on an FT/IR-4600 (JASCO) spectrometer using a diamond attenuated total reflectance (ATR) method. The spectral data are given as major peaks in wavenumbers (cm -1 ) and recorded in a spectral window of 4000-400 cm -1 . Elemental analyses were conducted using a PerkinElmer Series II CHNS/ O 2400 analyser. Powder X-ray diffraction (PXRD) spectra of 2 and 3 were recorded at room temperature (rt) and 1.0 × 10 -4 GPa (1 atm) using a Rigaku MiniFlex600 diffractometer (Cu Kα radiation: λ = 1.54184 Å). To determine the crystal structure of 3 by single-crystal X-ray diffraction (SCXRD), a single crystal was prepared by a liquid-liquid diffusion method using a ϕ4 glass tube. A minute amount of ascorbic acid was dissolved in methanol to prevent the oxidation of the iron(II) ion. A methanolic solution of (anilinium) 3 [Fe III (dto) 3 ]•3H 2 O and FeCl 2 •4H 2 O 1/1 (10 mmol L -1 , 0.50 mL) was introduced into the glass tube and layered with methanol (0.1 mL) for the purpose of buffering the reaction. A methanolic solution of n PrPh 3 P bromide (10 mmol L -1 , 0.50 mL) was layered on top. After 2 weeks at rt, black hexagonal prismatic crystals were obtained.

Taniai R., Endo T., Kanetomo T., Okazawa A., Kadobayashi H., Kawaguchi S.I, & Enomoto M. (2023). ⁵⁷Fe Mössbauer spectroscopy and high-pressure structural analysis for the mechanism of pressure-induced unique magnetic behaviour in (cation)[Fe^IIFe^III(dto)₃] (cation = Ph₄P and ⁿPrPh₃P; dto = 1,2-dithiooxalato). Dalton transactions (Cambridge, England : 2003), 52(24).

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Comprehensive Analytical Characterization

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Elemental analyses (C, H, N) were carried out on a Perkin Elmer Series II CHNS/O 2400 elemental analyzer. NMR spectra were recorded on a Bruker AV-500 NMR spectrometer. Fluorescence measurements were performed on a Shimadzu RF-5301/PC spectrofluorophotometer. The region between 200 and 400 nm was scanned for each sample. UV-Vis spectra were recorded on a TU-1901 ultraviolet spectrophotometer.

Yang Q.Y., Cao Q.Q., Qin Q.P., Deng C.X., Liang H, & Chen Z.F. (2018). Syntheses, Crystal Structures, and Antitumor Activities of Copper(II) and Nickel(II) Complexes with 2-((2-(Pyridin-2-yl)hydrazono)methyl)quinolin-8-ol. International Journal of Molecular Sciences, 19(7), 1874.

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Spectroscopic Characterization of Organic Compounds

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¹H and ¹³C NMR spectra were recorded on Bruker Avance II spectrometer in DMSO-d₆ or CDCl₃ (400 and 100 MHz, respectively) using Me₄Si as an internal standard. Mass experiments were performed on Shimadzu GCMS-QP2010 Ultra gas chromatograph operating at an ionization potential of 70 eV (EI). The IR data have been recorded on a Bruker Alpha (NPVO, ZnSe) FTIR spectrometer. Microanalyses were performed on PerkinElmer Series II CHNS/O 2400 elemental analyzer. The melting point was determined on a Stuart SMP 3 apparatus. The progress of the reactions and the purity of the compounds were monitored by TLC on TLC Silica gel 60 F245 Aluminum sheets (Merck KGaA) in EtOAc/hexane (1:2), EtOAc/hexane (1:1), CHCl₃/EtOH (1:1), CHCl₃/EtOH (20:1), CHCl₃/EtOH (50:1) system.

Efimov I.V., Shafikov M.Z., Beliaev N.A., Volkova N.N., Beryozkina T.V., Dehaen W., Fan Z., Grishko V.V., Lubec G., Slepukhin P.A, & Bakulev V.A. (2016). Combined experimental and theoretical studies of regio- and stereoselectivity in reactions of β-isoxazolyl- and β-imidazolyl enamines with nitrile oxides. Beilstein Journal of Organic Chemistry, 12, 2390-2401.

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Measuring Phytoplankton Growth Rates

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Triplicate sampling was performed once a day (Fig. 1). 12.64 mL of culture were filtered onto glass-fiber filters (Watman GF/C, Maidstone, UK) precombusted at 450 °C for 12 h. Samples were kept at 60 °C before analysis with a CHN analyzer (2400 Series II CHNS/O, Perkin Elmer, Norwalk, CT, USA). Continuous functions of Savitzky Golay filter [20 ] were fitted to carbon data series (µgC mL⁻¹), and the derivate was used to compute the net carbon specific growth rates µ (µgC µgC⁻¹ d⁻¹) according to the following equation:

μ = \frac{1}{X_{t_{2}}} (\frac{X_{t_{2}} - X_{t_{1}}}{(t_{2} - t_{1})} + D \cdot X_{t_{2}}),

where X is the particulate carbon concentration or carbon biomass concentration (µgC mL⁻¹) at time t₁ and t₂, respectively.

Bonnefond H., Moelants N., Talec A., Mayzaud P., Bernard O, & Sciandra A. (2017). Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation. Biotechnology for Biofuels, 10, 25.

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Degree of Substitution Analysis for Starch Derivatives

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The degree of substitution (DS)
of the products was determined by analyzing their nitrogen content
with an elemental analyzer (PerkinElmer 2400 Series II CHNS/O). The
DS was calculated using the following equation where N is the
percentage
of nitrogen, 162.15 is the molecular weight of the anhydroglucose
unit of starch, 157.24 is the molecular weight of the octyl carbamate
group, and 1401 is the atomic weight of nitrogen multiplied by 100.^{45 (link),65}

Sirviö J.A, & Heiskanen J.P. (2019). Carbamation of Starch with Amine Using Dimethyl Carbonate as Coupling Agent. ACS Omega, 4(13), 15702-15710.

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Growth and Pigment Analysis of Chlorella neogracilis

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C. neogracilis cells were counted and sized (equivalent spherical diameter) before and after culture dilution using a Beckman Multisizer 4 Coulter Counter. The concentrations of particulate C and N were determined daily. For particulate carbon and nitrogen, an aliquot of 10 mL of algal culture was filtered onto glass-fiber filters (Whathman GF/F 0.7μm, 25mm) pre-combusted at 500°C for 12 h. Filters were kept desiccated before elemental analysis with a CHN analyzer (2400 Series II CHNS⁄ O; Perkin Elmer, Norwalk, CT, USA). For pigment analysis, an aliquot of algal culture (5mL) was filtered onto glass-fiber filters (Whathman GF/F 0.7μm, 25mm), immediately flash-frozen in liquid nitrogen and stored at -80°C until analysis using the protocol described in Zapata, Rodriguez [29 ]. The xanthophyll de-epoxidation state (%) was calculated as Dt/(Dd + Dt)*100, where Dd is diadinoxanthin, the epoxidized form and Dt is diatoxanthin, the de-epoxidizedf [30 ].

Lacour T., Larivière J., Ferland J., Morin P.I., Grondin P.L., Donaher N., Cockshutt A., Campbell D.A, & Babin M. (2022). Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature. PLoS ONE, 17(9), e0272822.

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Elemental Analysis of Organic Compounds

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Quantitative elemental analysis was expressed as a percentage of the quantitative ratios between the atoms of the studied organic compound. A Perkin-Elmer 2400 Series II CHNS/O was used. The samples were weighed in tin microcans using a Perkin Elmer microbalance. The combustion temperature was set at 850° C and in the reduction zone the temperature was 500 °C. Cystine was used as an analytical standard. For each analyzed sample, two determinations were performed, using different amounts from the sample (~1.5 mg and ~2.5 mg). The result was calculated as the average of the two determinations.

Mititelu M., Moroșan E., Nicoară A.C., Secăreanu A.A., Musuc A.M., Atkinson I., Pandele Cusu J., Nițulescu G.M., Ozon E.A., Sarbu I, & Balaci T.D. (2022). Development of Immediate Release Tablets Containing Calcium Lactate Synthetized from Black Sea Mussel Shells. Marine Drugs, 20(1), 45.

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Comprehensive Characterization of Food Waste Biochar

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The heating value of the food waste-based biochar was measured using a bomb calorimeter (6400 Automatic Isoperibol Calorimeter, Parr, Moline, IL, USA). For chlorine measurement, an ion chromatograph (AQF-2100H, Mitsubishi Chemical Analytech Co., Ltd., Kanagawa, Japan) was used. Proximate analysis was conducted according to the American Society for Testing and Materials D7582 experimental standard. For ultimate analysis, 2400 series II CHNS/O (Perkin Elmer, Boston, MA, USA) was used. The heavy metals in bio-SRF were analyzed using a mercury analyzer (M7600, Teledyne, Thousand Oaks, CA, USA), and an inductively coupled plasma-optical emission spectrometer (Agilent 720, Agilent, Santa Clara, CA, USA) was used to analyze ionic components. To analyze saltwater quality, biochemical oxygen demand (BOD), suspended solids (SS), total nitrogen (TN), and total phosphorus (TP) were analyzed using a standard method [62 ]. Total organic carbon (TOC) was analyzed using a TOC-L analyzer (Shimadzu, Kyoto, Japan). Chloride, sulfate, phosphate, nitrate, nitrite, and bromide ions were analyzed using an ion chromatograph (ICS-1100, Thermo Fisher Scientific, Waltham, MA, USA). The remaining volatile organic compounds (VOCs) were analyzed using a high-sensitivity (HS)–gas chromatography/mass spectrometer (QP2020NX, Shimadzu, Kyoto, Japan).

Ahn K.H., Shin D.C., Lee Y.E., Jeong Y., Jung J, & Kim I.T. (2023). Biochar Production and Demineralization Characteristics of Food Waste for Fuel Conversion. Molecules, 28(16), 6114.

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