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Pgl4.23 reporter construct

Manufactured by Promega
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The PGL4.23 reporter construct is a plasmid-based genetic tool used in molecular biology research. It contains a luciferase gene that can be used to generate a luminescent signal, allowing for the quantification of gene expression or promoter activity in various experimental systems.

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Lab products found in correlation

Pgl4.23 luc2 pmin luciferase reporter construct, by Promega (2 mentions) Prl cmv renilla control plasmid, by Promega (2 mentions) Dual glo luciferase assay system, by Promega (2 mentions)

Close spelling variants of this product

PGL4.23[luc2/pmin] luciferase reporter construct

2 protocols using pgl4.23 reporter construct

1

Transcriptional Impact of Somatic Mutations

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To examine the effects of recurrent somatic mutations on transcription, wild-type and mutant regions 201bp in length and centered on each mutation were synthesized and cloned into the KpnI and NheI site of the pGL4.23[luc2/pmin] luciferase reporter construct (Promega). Lung adenocarcinoma (NCI-H1437), esophageal adenocarcinoma (KYSE-450) and bladder carcinoma (Ku-19-19) cells, growing in 96-well plates, were transfected in quadruplicate with 200ng of the pGL4.23 reporter construct and 40ng of the pRL-CMV Renilla control plasmid (Promega). Forty-eight hours post-transfection luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega). Statistically significant differences in the relative luciferase activities between wild-type and mutant regions were determined using a two-sided Student’s t-Test, assuming equal variance. Visually the variance appears equal between the tested data and there is no biologically reason they should be different.
Melton C., Reuter J.A., Spacek D.V, & Snyder M. (2015). Recurrent Somatic Mutations in Regulatory Regions of Human Cancer Genomes. Nature genetics, 47(7), 710-716.
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2

Transcriptional Impact of Somatic Mutations

Check if the same lab product or an alternative is used in the 5 most similar protocols
To examine the effects of recurrent somatic mutations on transcription, wild-type and mutant regions 201bp in length and centered on each mutation were synthesized and cloned into the KpnI and NheI site of the pGL4.23[luc2/pmin] luciferase reporter construct (Promega). Lung adenocarcinoma (NCI-H1437), esophageal adenocarcinoma (KYSE-450) and bladder carcinoma (Ku-19-19) cells, growing in 96-well plates, were transfected in quadruplicate with 200ng of the pGL4.23 reporter construct and 40ng of the pRL-CMV Renilla control plasmid (Promega). Forty-eight hours post-transfection luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega). Statistically significant differences in the relative luciferase activities between wild-type and mutant regions were determined using a two-sided Student’s t-Test, assuming equal variance. Visually the variance appears equal between the tested data and there is no biologically reason they should be different.
Melton C., Reuter J.A., Spacek D.V, & Snyder M. (2015). Recurrent Somatic Mutations in Regulatory Regions of Human Cancer Genomes. Nature genetics, 47(7), 710-716.
+ Open protocol
+ Expand

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