PGE1 was purchased from Zhuhai Schwarz Pharma Co., Ltd. (Zhuhai, China). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco Life Technologies (Carlsbad, CA, USA). Penicillin-streptomycin was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). A Hoechst 33258 Staining kit and propidium iodide (PI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies targeting Bcl-2 (cat. no. 2870), Bax (cat. no. 2772), and caspase-3 (cat. no. 9662), were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA) and were used at a dilution of 1:1,000. A Bicinchoninic Acid (BCA) Protein Assay kit was purchased from Kangchen Bio-tech (Shanghai, China). Enhanced Chemiluminescence (ECL) solution was purchased from KeyGen Biotech Co., Ltd. (Nanjing, China). Fluorescein isothiocyanate (FITC)-labeled Annexin V and anti-rat CD90 (cat. no. 11-0900-81), CD45 (cat. no. 17-0461-80), CD11b/c (cat. no. 12-0110-80), and CD29 (cat. no. 46-0291-80), antibodies were purchased from BD Biosciences (San Jose, CA, USA).
Hoechst 33258 staining kit
Manufactured by Merck Group
Sourced in United States
Hoechst 33258 Staining Kit is a fluorescent dye-based laboratory reagent used for the detection and visualization of DNA in biological samples. The kit contains the Hoechst 33258 dye, which selectively binds to adenine-thymine (A-T) rich regions of DNA, allowing for the identification and quantification of DNA content in cells or tissues.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Enhanced chemiluminescence ecl solution, by Keygen Biotech (1 mentions)
Inverted fluorescence microscope, by Olympus (1 mentions)
3 protocols using hoechst 33258 staining kit
1
Mesenchymal Stem Cell Apoptosis Assay
2
Apoptosis Induction Assay with Cinobufagin and CDDP
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143B cells were seeded in 6-well plates and treated with cinobufagin and CDDP alone and in combination for 48 h. Then, each well were stained according to the protocol of Hoechst 33258 Staining Kit (Sigma). After incubation for 30 minutes away from light, the cells were photographed with an inverted fluorescence microscope. bright-blue fluorescent and condensed nuclei were designated as apoptotic cells.
3
Hoechst 33258 Staining for Apoptosis
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Hoechst 33258 Staining Kit (Sigma-Aldrich, St. Louis, MO, USA) was used for this assay. The cells were incubated in a 6-well plate for 24 h and further treated with or without baicalein for 24 h. Subsequently, cells were stained according to the instructions and then observed under an inverted fluorescence microscope (Olympus) (×200). Cells were designated as apoptotic cells based on nuclear morphology changes, such as bright-blue fluorescent and condensed nuclei.
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