Lipid peroxidation levels were measured by levels of malondialdehyde (MDA) using thiobarbituric acid method [27 (link)]. Kidney tissues were homogenized with 0.15M KCl (Duksan, Gyeonggi-do, South Korea) solution. 0.2ml of homogenous kidney tissue was added to 0.2ml of 8.1% SDS (Sigma-Aldrich, St. Louis, MO., USA) and incubated at room temperature for 10min. 3ml of 20% acetic acid (Duksan, Gyeonggi-do, South Korea)-0.8% TBA mixture (Lancaster Synthesis, Morecambe, England) (1:1, v/v) and 0.6ml of distilled water were added. The reaction mixture was heated in a water bath at 95℃ for 1h, and then cooled by tap water immediately. To each tube, 1ml distilled water and 5ml of n-butanol (Duksan, Gyeonggi-do, South Korea) and pyridine (Duksan, Gyeonggi-do, south Korea) (15:1, v/v) were added and shaken using a vortex. After centrifuging at 4,000 rpm for 10 min, the pink supernatant was measured at 532 nm using ELISA reader (BIO-TEK instruments, Winooski, VT, USA) with 1,1,3,3-tetramethoxypropane (Sigma-Aldrich) as a standard.
Pyridine
Manufactured by Duksan Pure Chemicals
Sourced in Cameroon
Pyridine is a colorless, volatile, water-soluble organic compound. It is a heterocyclic aromatic compound with the chemical formula C₅H₅N. Pyridine serves as a core building block for various chemical substances and is commonly used in the synthesis of pharmaceutical and agricultural products.
Automatically generated - may contain errors
Lab products found in correlation
N butanol, by Duksan Pure Chemicals (1 mentions)
3 tetramethoxypropane, by Merck Group (1 mentions)
Elisa reader, by Agilent Technologies (1 mentions)
Potassium chloride (kcl), by Duksan Pure Chemicals (1 mentions)
Acetic anhydride, by Duksan Pure Chemicals (1 mentions)
Triethylamine, by Duksan Pure Chemicals (1 mentions)
4 4 oxydianiline, by Merck Group (1 mentions)
Pyromellitic dianhydride, by Merck Group (1 mentions)
3 protocols using pyridine
1
Malondialdehyde Quantification in Kidney Tissue
2
Freeze-Dried Pickering HIPE Synthesis
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Pyromellitic dianhydride (PMDA) and 4,4′-oxydianiline (ODA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1-Methyl-2-pyrrolidone (NMP), pyridine, acetic anhydride, triethylamine (TEA), and cyclohexane were purchased from DUKSAN (Ansan, Gyeonggi-do, Korea). Acetone was purchased from SK Chemicals (Seongnam, Korea). An aluminum mold with a cubic hole (20 mm × 20 mm × 20 mm) was made and used to freeze-dry the Pickering HIPEs.
3
Synthesis of DAn Compound using AnDA Method
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DAn was donated by Prof. K. Kudo (Tokyo University). AnDA was prepared by using a previously reported method [40 (link)]. GO sheets were purchased from Dae Joo Electronic Materials Co., Ltd., Gyeonggi-do, Korea, and used without purification. N,N-dimethylacetamide (DMAc)), acetic anhydride and pyridine were purchased from Duksan Pure Chemicals Co., Ltd., Seoul, Korea. The rest of the chemicals were of analytical grade and used without further purification.
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