Peripheral blood (150 µl/mouse) was harvested from the retro-orbital venous plexus and collected in RPMI 1640 containing 2% EDTA. Hematological examinations were performed using a Coulter AcT Diff hematology analyzer (Beckman Coulter, Inc., Brea, CA, USA) or a HEMAVET 950 multispecies hematology instrument (DREW Scientific, Inc., Dallas, TX, USA).
Coulter ac t diff hematology analyzer
Manufactured by Beckman Coulter
Sourced in United States, Australia
The Coulter Ac·T diff Hematology Analyzer is a compact, automated hematology instrument designed for clinical laboratory use. It provides a comprehensive analysis of blood samples, including the measurement of red blood cells, white blood cells, and platelets, as well as the differentiation of various white blood cell types.
Automatically generated - may contain errors
Lab products found in correlation
Hemavet 950, by Drew Scientific (1 mentions)
Iron tibc reagent kit, by Horiba (1 mentions)
Au480 chemistry analyzer, by Beckman Coulter (1 mentions)
Iron tibc reagent set, by Horiba (1 mentions)
3 ml syringe, by BD (1 mentions)
S monovette serum gel tubes, by Sarstedt (1 mentions)
S monovette k3 edta tubes, by Sarstedt (1 mentions)
Ficoll paque plus, by GE Healthcare (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Leucosep tube, by Greiner (1 mentions)
Summit 4, by Agilent Technologies (1 mentions)
Facs lysing solution, by BD (1 mentions)
Lsrii fortessa, by BD (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin g, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
13 protocols using coulter ac t diff hematology analyzer
1
Peripheral Blood Hematological Analysis
2
Hematology and Iron Assays in Euthanized Subjects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematological parameters were assessed immediately after euthanasia using a Coulter Ac·T diff Hematology Analyzer (Beckman Coulter, Gladesville, Australia). Serum iron parameters were determined using the Iron/TIBC Reagent Kit (Pointe Scientific, Canton, MI) as previously described (Frazer et al. 2017 (link)). Tissue iron levels were assayed by colorimetric assay as previously described (Torrance and Bothwell 1968 (link)).
3
Biomarker Analyses for Heatstroke Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Prior to and immediately after the HTT, 4 mL, venous blood samples were obtained from the anti-cubital fossa for haematological, biochemical and hormonal analyses while participants were sitting upright and resting. Blood was collected in EDTA (Ethylenediaminetetraacetic acid) tubes and analysed for white blood cell count, lymphocytes, monocytes and platelets using an automated haematology analyser (Coulter AcT diff Hematology Analyzer, Beckman Coulter, New South Wales, Australia). The biochemical and hormonal analyses samples were collected using a serum separator collection tube and centrifuged at 4500 rpm for 10 min for serum separation. The serum was frozen at −80 °C for subsequent sodium (Na), potassium (K), chloride (Cl), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatine kinase (CK) and creatinine analyses (AU480 Chemistry Analyzer, Beckman Coulter, New South Wales, Australia). Hormonal analysis for serum cortisol utilised the Access reagent kit (Access 2 Immunoassay System, Beckman Coulter, New South Wales, Australia). The selected biomarkers are investigated after a heat stroke event and are useful indicators of injury severity and clinical recovery [11 (link)].
4
Automated Leukocyte Differential Counting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total WBC and differential leukocyte counts were determined according to the manufacturer's protocol using an automated Coulter Ac.T diff Hematology analyzer (Beckman Coulter, Inc.).
5
Exercise-Induced Plasma Volume Shifts
6
Hematological and Iron Biomarker Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematological parameters were measured using a Coulter Ac·T diff Hematology Analyzer (Beckman Coulter, Gladesville, Australia). Total serum iron and transferrin saturation were measured using the Iron/TIBC Reagent Set (Pointe Scientific, Canton, MI, USA) as previously described [39 (link)]. Maternal liver nonheme iron was determined using a colorimetric assay [40 (link)].
7
Blood Collection and Preparation
8
Blood Sample Collection and PBMC Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We obtained approximately 30 ml of blood in S-Monovette K3 EDTA tubes and 7 ml in S-Monovette Serum-Gel tubes (Sarstedt). All samples were collected in the morning (8:00 a.m.). PBMCs were then isolated using a Ficoll–Hypaque gradient as described (17 (link)), aliquoted in RPMI containing 10% DMSO and 25% FCS at 1 × 107 cells/ml, gradually cooled down to −80°C in a Mr. Frosty for 18 h and stored in liquid nitrogen until assayed. A small amount of whole blood (50 µl) was used for total and differential leukocyte counts using a Coulter Ac·T Diff hematology analyzer (Beckman Coulter). All other assays were performed using cryopreserved PBMCs or serum with one subject from each group run in parallel to control systematic variation in reagents used.
9
Cryopreservation of PBMC Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Overall, PBMCs were isolated from blood samples of 156 pediatric HCT recipients drawn just before the first vaccination; the remaining 14 were excluded from analysis because of low cell viability (n = 10) or lack of a baseline specimen (n = 4). Whole blood was collected into prefilled Leucosep tubes (Greiner Bio-One, Monroe, NC) containing Ficoll-Paque PLUS (GE HealthCare, Piscataway, NJ) and processed on the day of collection. Whole blood was centrifuged at 800g for 15 minutes. The buffy coat was removed, pelleted by low-speed centrifugation (400g), washed 2 times with phosphate-buffered saline (PBS), and counted on a Coulter Ac·T diff Hematology Analyzer (Beckman Coulter, Brea, CA). The cells were washed a third time and resuspended in media composed of 90% fetal bovine serum (Atlanta Biologicals, Norcross, GA) containing 10% dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MO). Cells were cryopreserved at −80°C in a StrataCooler (Fisher Scientific, Hampton, NH) overnight and transferred to liquid nitrogen storage.
10
Quantification of Natural Killer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete blood counts were obtained from all blood samples using a COULTER Ac•T diff Hematology Analyzer (Beckman Coulter, Inc, Brea, CA). Whole blood samples were stained using fluorescently labeled monoclonal antibodies for the cell surface markers CD3, CD16, and CD56. The specific fluorescent labels were fluorescein isothyocyanate for the CD3 marker, phycoerythrin for the CD16 marker, and allophycocyanin for the CD56 marker. Fluorescent staining was done to identify the proportion of lymphocytes that express the CD3 -CD16 + CD56 + phenotype that is characteristic of NK cells. Stained samples were further prepared for flow cytometric analysis according to procedures adapted from protocols made available by Becton Dickinson. 30 Samples were fixed in a 1% paraformaldehyde solution and analyzed within 24 hours of preparation using a CyAn 3 laser/9 color flow cytometer (Beckman Coulter, Inc). Flow cytometry data were viewed and analyzed using Summit 4.3 software (Dako North America, Inc, Carpinteria, CA). Absolute NK cell counts were calculated by multiplying total lymphocyte counts by the NK cell proportions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!