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Antibodies recognizing dyskerin and gapdh

Manufactured by Santa Cruz Biotechnology

Antibodies recognizing dyskerin and GAPDH are laboratory tools used to detect and study the presence and distribution of these proteins in biological samples. Dyskerin is a protein involved in telomere maintenance and ribosome biogenesis, while GAPDH is a commonly used housekeeping gene involved in glycolysis. These antibodies can be used in various experimental techniques, such as Western blotting, immunohistochemistry, or immunofluorescence, to identify and quantify the target proteins.

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2 protocols using antibodies recognizing dyskerin and gapdh

1

Cellular Toxicity Screening Assay

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Aphidicolin, doxorubicin, rapamycin, chloroquine, trichostatin A, suberanilohydroxamic acid, etoposide, tunicamycin, MG132, nocodazole, and roscovitine were obtained from Sigma-Aldrich (St. Louis, MO). Thapsigargin (R&D Systems, Minneapolis, MN) was generously provided by Dr. Kelly Jordan-Sciutto. Aggregatibacter actinomycetemcomitans cytolethal distending toxin B subunit was obtained from Dr. Bruce Shenker. Antibodies recognizing dyskerin and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). GAR1 and NHP2 antibodies were obtained from Proteintech (Chicago, IL). NOP10, fibrillarin, and LC3B antibodies were from Abcam (Cambridge, MA). All other antibodies, including secondary antibodies, were obtained from Cell Signaling Technology (Danvers, MA).
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2

Doxorubicin and Epigenetic Modulators Protocol

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Doxorubicin, neocarzinostatin, trichostatin A, suberoylanilide hydroxamic acid (SAHA), chloroquine and bafilomycin were obtained from Sigma-Aldrich (St. Louis, MO). Antibodies recognizing dyskerin and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). All other antibodies, including secondary antibodies, were obtained from Cell Signaling Technology (Danvers, MA).
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