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Accu chek aviva blood glucose meter

Manufactured by Roche
Sourced in Switzerland

The Accu-Chek Aviva is a blood glucose meter manufactured by Roche. It is designed to measure and display the user's blood glucose levels. The device requires a small blood sample, which is analyzed to provide the glucose reading.

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12 protocols using accu chek aviva blood glucose meter

1

Post-Mortem Blood Collection and Analysis

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Immediately post mortem, blood was collected from the aorta. The blood was transferred to an Eppendorf tube, allowed to coagulate at room temperature for 30 min and centrifuged for 10 min at room temperature, 1300 rcf. The plasma was then collected and stored at −80 °C until analysis by Sentrallaboratoriet NMBU Veterinærhøgskolen (Oslo, Norway). Blood glucose levels were measured by Accu-Chek Aviva blood glucose meter (Roche, Basel, Switzerland) by one drop of freshly collected blood.
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2

Metabolic Profiling of Diabetic Mice

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A total of 38 mice were analyzed in this study (db/db n=11; wt/db n=16; wt n=11). Measurements commenced at 4 weeks of age immediately following completion of weaning. Mice were placed in metabolic cages (Nalgene) every 4 weeks for 24 h urine collection and provided with food and water ad libitum. The total volume of urine was recorded and stored at −20 °C. Immediately following urine collection, mice were placed in clean cages and fasted for 6 h (10:30–16:30). Blood was collected after fasting by a small incision at the tip of the tail to measure FPG (Accu-Chek Aviva Blood Glucose Meter, Roche Diagnostics). 7μL of blood was collected for HbA1c measurement using the Mouse Hemoglobin A1c assay (Crystal Chem Inc.). Equal numbers of male and female mice were used. No sex specific differences in CEdG or FPG levels were observed throughout the course of the study (Supporting Information Figure S1). For tissue collection, mice were euthanized and perfused with PBS (pH 8) to obtain liver, kidney, pancreas, and colon tissues. Samples were then immediately flash-frozen in liquid N2 and stored at −80 °C.
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3

Glucose and Insulin Tolerance Tests in HFD Mice

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Glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed using HFD‐fed Wwp1 WT and KO mice at 13–15 weeks old. Prior to GTT and ITT, mice were fasted for 24 h. d‐glucose (1.0 g/kg body weight, Wako) or insulin (1.0 U/mL body weight, Wako) were injected intraperitoneally for GTT or ITT, respectively. Next, serial blood sampling from the tail vein was performed at 0, 30, 60, and 120 min after injection. Blood glucose levels were measured using an Accu‐chek® aviva blood glucose meter (Roche).
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4

Glucose Monitoring via Tail Puncture

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Glucose metering was determined by taking advantage of the Accu-Chek Aviva blood glucose meter (Roche, Mannheim, Germany). Capillary blood droplets were drawn by puncture of the tail.
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5

Intraperitoneal Glucose Tolerance Test

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To determine fasting blood glucose levels, 1 mg/g glucose solution (D-Glucose, Sigma Aldrich) was injected intraperitoneally into the mice after a 16 hour fasting period. Blood glucose was measured from the tail vein immediately prior to glucose administration and after 20, 40 and 60 minutes with a glucometer (ACCU-CHEK Aviva blood glucose meter, Roche).
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6

Mouse Model of Hyperglycemia and BCG

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BALB/c male mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and housed at five animals per cage at the MGH animal facilities (four animals per cage after they reached a weight of >25 g). Studies had full IACUC approval under protocol #2008N000176 and involved at all times the ethical treatment of animals. We determined HbA1c from mouse blood samples using the A1CNow + kit from PTS Diagnostics (Indianapolis, IN). For mouse blood sugars we used an ACCU-CHEK Aviva blood glucose meter (Roche, Indianapolis, IN). BCG was administered by hind footpad injection (0.10 mg BCG in 25 μL saline in one footpad). Induction of hyperglycemia was performed using a single intraperitoneal injection of Streptozotocin (Sigma-Aldrich, Milwaukee, WI) at 150 mg/kg in PBS.
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7

Glucose and Insulin Tolerance Tests

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To determine fasting blood glucose levels, 1 mg/g glucose solution (D-Glucose; Sigma-Aldrich) was injected intraperitoneally into the mice after a 16-hour fasting period. Blood glucose was measured from the tail vein immediately prior to glucose administration and after 20, 40 and 60 minutes with a glucometer (ACCU-CHEK Aviva blood glucose meter; Roche). For insulin-tolerance testing, the mice were fasted for 16 hours. Blood glucose was first measured from the tail vein, then the mice received an intraperitoneal injection of insulin (0.75 U/kg; Sigma-Aldrich) and blood glucose was measured again at time points of 20, 50 and 80 minutes after injection.
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8

Glucose and Insulin Tolerance Tests in Wwp1 Mice

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GTT and ITT were performed in HFD‐fed Wwp1 WT and KO mice of 13–15 weeks old. Prior to GTT and ITT, mice were fasted for 24 and 8 h, respectively. D‐glucose (1.0 kg per body weight, Wako) or insulin (1.0 U per kg body weight, Wako) were injected intraperitoneally for GTT and ITT, respectively. Next, serial blood sampling from the tail vein was performed at 0, 15, 30, 60, and 120 min after injection. Blood glucose levels were measured using an Accu‐Chek® Aviva blood glucose meter (Roche, Basel, Switzerland).
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9

Fasting Glucose and Insulin Levels in Mice

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Mice were fasted for 12 h before examination of fasting glucose and insulin levels (n = 5). Blood samples from mice aged 8 weeks to 18 weeks were collected from the tail vein for the detection of glucose levels using an Accu-Chek Aviva blood glucose meter (Roche, Basel, Switzerland). Fasting insulin levels were examined with a Mouse Insulin ELISA Detection Kit (Mercodia, Uppsala, Sweden) according to the manufacturer’s instructions. The absorbance of samples was measured by a BioTek 800 TS Absorbance Reader (BioTek Instruments, Inc., USA).
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10

Stress-Minimized Blood Measurement

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Blood pressure measurement was performed by non-invasive tail cuff (CODA High Throughput System, Kent Scientific Corp., Torrington, USA). Blood glucose was measured by taking capillary blood droplets from tail puncture (Accu-Chek Aviva blood glucose meter, Roche, Mannheim, DEU). The measurements are carried out by animal keepers with decades of experience in a quiet environment to keep the stress for the animals as low as possible.
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