Anti-mouse CD140b (catalog 136002, RRID: AB_1953332), APC anti-mouse CD140b antibody (catalog 136008, RRID: AB_2268091), Alexa Fluor 647 anti-human CD31 antibody (catalog 303111, RRID:AB_493077), Alexa Fluor 488 anti-human CD31 antibody (catalog 303109, RRID:AB_493075), APC anti-human CD140b (PDGFRβ) antibody (catalog 323608, RRID:AB_2162787), Brilliant Violet 421 anti-mouse CD45 antibody (catalog 103133, RRID:AB_10001045), BV421 anti-human CD117 (C-kit) antibody (catalog 313215, RRID:AB_10896056), BV421 anti-mouse CD117 (catalog 560557, RRID:AB_1645258), PE/Cy5 anti-human CD45 (catalog 304010, RRID:AB_314398), APC anti-mouse FCεRIα (catalog 134316, RRID:AB_10640121), Brilliant Violet 711 anti-human TNF-α antibody (catalog 502940, RRID AB_2563885), and APC anti-mouse TER-119/erythroid cells antibody (catalog 116212 RRID AB_313713) were from BioLegend. Mouse/rat CD31/PECAM-1 antibody (catalog AF3628, RRID:AB_2161028), mouse mast cell protease-6/Mcpt6 antibody (catalog MAB3736, RRID:AB_2240825), and BV510 anti-human FCεRIα (catalog 334626, RRID:AB_2564291) were from R&D Systems
Alexa fluor 488 anti human cd31 antibody
Manufactured by BioLegend
Alexa Fluor 488 anti-human CD31 antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the CD31 protein, also known as platelet endothelial cell adhesion molecule (PECAM-1). CD31 is a cell surface marker expressed on endothelial cells, platelets, and some immune cells. This antibody can be used to detect and analyze CD31-positive cells in various applications, such as flow cytometry and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Apc anti mouse fcεriα, by BioLegend (1 mentions)
Apc anti mouse cd140b antibody, by BioLegend (1 mentions)
Alexa fluor 647 anti human cd31 antibody, by BioLegend (1 mentions)
Pe cy5 anti human cd45, by BioLegend (1 mentions)
Anti human cd326 epcam antibody, by BioLegend (1 mentions)
C2 laser scanning confocal microscope, by Nikon (1 mentions)
2 protocols using alexa fluor 488 anti human cd31 antibody
1
Multicolor Flow Cytometry Analysis of Mouse and Human Cells
2
Multimodal Imaging of Vascular and Cell Structures
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The vessel
sample was fixed with 4% of paraformaldehyde in PBS overnight at 4
°C and incubated with a 1:50 diluted Alexa Fluor 488 antihuman
CD31 antibody (BioLegend) and 50 μg/mL of DAPI overnight at
4 °C. The fibroblast-laden beads were fixed and incubated with
a human α-smooth muscle actin (α-SMA) Alexa Fluor 594-conjugated
antibody (R&D System).71 (link) The HCT-116-laden
beads were fixed and incubated with an antihuman CD326 (EpCAM) antibody
(BioLegend).72 (link) The vessel network sample
was fixed and incubated with an Alexa Fluor 488 antimouse CD31 (PECAM)
antibody (BioLegend).73 (link) All of the fluorescent
images and confocal scanning were acquired with a Nikon C2+ laser
scanning confocal microscope in the Health Research Hub Center, Lehigh
University.
sample was fixed with 4% of paraformaldehyde in PBS overnight at 4
°C and incubated with a 1:50 diluted Alexa Fluor 488 antihuman
CD31 antibody (BioLegend) and 50 μg/mL of DAPI overnight at
4 °C. The fibroblast-laden beads were fixed and incubated with
a human α-smooth muscle actin (α-SMA) Alexa Fluor 594-conjugated
antibody (R&D System).71 (link) The HCT-116-laden
beads were fixed and incubated with an antihuman CD326 (EpCAM) antibody
(BioLegend).72 (link) The vessel network sample
was fixed and incubated with an Alexa Fluor 488 antimouse CD31 (PECAM)
antibody (BioLegend).73 (link) All of the fluorescent
images and confocal scanning were acquired with a Nikon C2+ laser
scanning confocal microscope in the Health Research Hub Center, Lehigh
University.
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