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Quantichrom kits

Manufactured by BioAssay Systems
Sourced in United States

QuantiChrom kits are colorimetric assay kits designed to quantify various analytes in biological samples. The kits utilize a simple and rapid procedure to directly measure the target analyte concentration using a spectrophotometer or microplate reader. They provide a fast, sensitive, and cost-effective solution for researchers to determine the levels of specific biomolecules in their experiments.

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2 protocols using quantichrom kits

1

Comprehensive Plasma and Urine Biochemistry Analysis

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The concentrations of phosphate, calcium, and creatinine in arterial blood plasma and urine were determined using the corresponding QuantiChrom kits (DIPI-500, DICA-500, and DICT-500) from BioAssay Systems. Urine was collected for 24 h in metabolic cages, and therefore acute food switches for 4 h were not analyzed.
The plasma concentrations of several hormones were determined by ELISA using the following kits and according to the manufacturer’s protocols: rat intact PTH (Immunotopics, cat. 60-2500), FGF-23 (Kainos Laboratories, CY-4000), Rat Klotho KLOT (BlueGene, E02K0027), secreted frizzled-related protein 4 (SFRP4; Biomatik, EKU07221), matrix extracellular phosphoglycoprotein (MEPE; Cloud-Clone Corp., SEB232Ra), dopamine (Cloud-Clone Corp, CEA851Ge), rat CORT for corticosterone (Elabscience, EL-0160), rat thyroxine (T4) ELISA Kit (Cusabio, E05082r), General Calcitriol ELISA Kit (EIAab, E0467Ge), and Insulin kit (Could-Clone Corp, CEA448Ra).
Total nicotinamide adenine dinucleotide was determined with an NAD+/NADH Assay Kit (Abcam, ab65348) in 20 mg of kidney cortex and jejunum scrapings, following the kit’s procedure. Optical density was determined at 450 nm with a DTX 880 Multimode Detector (Beckman Coulter).
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2

Plasma Biomarker Quantification

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Plasma BUN and creatinine levels were determined using the respective Quantichrom kits (BioAssay Systems, Hayward, CA, USA), according to the manufacturer’s protocols.
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