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The KO-KSR is a laboratory equipment designed for cell culture applications. It serves as a serum-free medium supplement that supports the growth and maintenance of embryonic stem cells and induced pluripotent stem cells. The core function of the KO-KSR is to provide a chemically defined, animal-component-free environment for the cultivation of these specialized cell lines.

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Lab products found in correlation

Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) Matrigel, by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) D1530, by Merck Group (2 mentions) Pz0162, by Merck Group (2 mentions) Knockout d mem based medium, by Thermo Fisher Scientific (2 mentions)

2 protocols using ko ksr

1

Manipulation of Mll1 in Murine ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
All ESC lines in this study were adapted to the KnockOut D-MEM-based medium (Thermo Fisher Scientific) supplemented with 2 mM Glutamax (Thermo Fisher Scientific), 1X nonessential amino acids (Thermo Fisher Scientific), 1X sodium pyruvate (Thermo Fisher Scientific), 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), 103 U ml−1 LIF (Millipore, ESG1107). Cells were cultured with either 20% FBS (Atlas Biologicals, catalog number: F-0500-D) if grown on top of MEF feeder cells or 15% KO-KSR (Thermo Fisher Scientific 10828028) and 5% FBS if grown on Matrigel (Thermo Fisher Scientific, A1413202) coated plates, or 20% KO-KSR if grown under 2i condition. 2i consists of 1µM PD0325901 (Sigma Aldrich, PZ0162) and 3µM CHIR99021 (Sigma Aldrich, SML1046). 1α,25-Dihydroxyvitamin D3 (Sigma Aldrich, D1530) is used at a final concentration of 1 µM. Mll1 was deleted by treating Mll1fl/fl-Cre-ERTM ESCs with 5µM 4-OHT for 48hrs. MLL1 inhibition was done by MM-401 treatment at a final concentration of 100 µM (Zhang et al., 2016b (link)).
Zhang H., Khoa L.T., Mao F., Xu H., Zhou B., Han Y., O’Leary M., Nusrat A., Wang L., Saunders T.L, & Dou Y. (2019). MLL1 Inhibition and Vitamin D Signaling Cooperate to Facilitate the Expanded Pluripotency State. Cell reports, 29(9), 2659-2671.e6.
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2

Manipulation of Mll1 in Murine ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
All ESC lines in this study were adapted to the KnockOut D-MEM-based medium (Thermo Fisher Scientific) supplemented with 2 mM Glutamax (Thermo Fisher Scientific), 1X nonessential amino acids (Thermo Fisher Scientific), 1X sodium pyruvate (Thermo Fisher Scientific), 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), 103 U ml−1 LIF (Millipore, ESG1107). Cells were cultured with either 20% FBS (Atlas Biologicals, catalog number: F-0500-D) if grown on top of MEF feeder cells or 15% KO-KSR (Thermo Fisher Scientific 10828028) and 5% FBS if grown on Matrigel (Thermo Fisher Scientific, A1413202) coated plates, or 20% KO-KSR if grown under 2i condition. 2i consists of 1µM PD0325901 (Sigma Aldrich, PZ0162) and 3µM CHIR99021 (Sigma Aldrich, SML1046). 1α,25-Dihydroxyvitamin D3 (Sigma Aldrich, D1530) is used at a final concentration of 1 µM. Mll1 was deleted by treating Mll1fl/fl-Cre-ERTM ESCs with 5µM 4-OHT for 48hrs. MLL1 inhibition was done by MM-401 treatment at a final concentration of 100 µM (Zhang et al., 2016b (link)).
Zhang H., Khoa L.T., Mao F., Xu H., Zhou B., Han Y., O’Leary M., Nusrat A., Wang L., Saunders T.L, & Dou Y. (2019). MLL1 Inhibition and Vitamin D Signaling Cooperate to Facilitate the Expanded Pluripotency State. Cell reports, 29(9), 2659-2671.e6.
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