Flexcontrol software 3

Identification of the strain collection of W. chitiniclastica using MALDI TOF MS was performed as previously described (Schröttner et al., 2014 (link), 2016 (link)). In brief, strains were grown on Colombia blood agar plates for 24 h at 37°C. Single colonies were picked and plated on a 96-well steel target. Bacteria were dried on a laboratory workbench for 10 min and then overlaid with a 1 μl matrix (α-Cyano-4-hydroxycinnamic acid, Bruker Daltonik, Bremen, Germany) dissolved in an organic solvent. Subsequently, MALDI-TOF MS analyses were performed using flexControl software 3.1 (Bruker Daltonik, Bremen, Germany) following the manufacturer’s guidelines. Results are displayed in Table 3.

All isolates were grown on Columbia blood agar plates (bioMérieux, Nürtingen, Germany) for 18 h at 37 °C. Then, a single colony was picked and plated on a 96-spot steel target (Bruker Daltonik, Bremen, Germany) and superimposed by α-Cyano-4-hydroxycinnamic acid (Bruker Daltonik, Bremen, Germany), which was used as an analytical matrix. The spectra were analyzed using a microflex MALDI-TOF MS system (Bruker Daltonik, Bremen, Germany) applying the flexControl software 3.1 (Bruker Daltonik, Bremen, Germany). This method has been described in more detail in a previous publication by our group [16 (link)]. For the MALDI-TOF MS experiments, calibration and positive control were performed with the BRUKER bacterial test standard (BTS).