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2 protocols using anti atf5

1

Analyzing Transcriptional Regulators in Mycobacterial Infection

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Anti-LXRα (ab41902 Abcam), anti-ATF1 (sc270, Santa Cruz), anti-ATF3 (sc188, Santa Cruz), anti-ATF5 (sc377168, Santa Cruz), anti-RXRA (sc553, Santa Cruz), anti-RARA (sc551, Santa Cruz), anti-MEF2A (ab7606. Abcam), anti-MEF2C (ab64644, Abcam), anti-LC3 (NB100-2220, Novus), anti-β-actin (sc-1616, Santa Cruz), anti-H3K27ac (ab4729),anti-NCoR (ab24552), anti-KAT3B/p300 (ab14984), anti-KAT13A/SRC1/NCoA1 (ab84), anti-H3K4me1 (ab8895, abcam), anti-PPARγ (ab41928, Abcam), anti-Mtb-FITC (ab20962, Abcam), anti-rabbit Alexa647 (A-21245, Life Technologies), Prolong Gold with Dapi (Life Technologies), anti-rabbit-Alexa488 (Life Technologies), Bodipy 493 (Life Technologies). TO901317, ATRA and BAX Inhibiting Peptide V5 were bought from Sigma and resupended in DMSO. Nelfinavir (Nel) and Ritonavir (Rit) were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases. Auto Histone ChIP-seq Kit (Protein A) was bought from Diagenode.
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2

Investigating Cellular Stress Responses

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Reagents used in this study include the following: Methylthiazolyldiphenyl-tetrazolium bromide (MTT)(S6821), PKI-402(S2739), cycloheximide (CHX)(NSC-185) and Thapsigargin (Tg) (S7895)were commercially sourced from Selleckchem (Houston, TX, USA), anti-G3BP1 (13057-2-AP), anti-eIF2α (11233-1-AP), anti-4EBP1 (60246-1-Ig), anti-caspase-9(66169-1-Ig), anti-Bcl-2 (26593-1-AP), anti-Bax (50599-2-Ig), anti-Hsp60 (15282-1-AP), anti-Lonp (66043-1-Ig), anti-Clpp (15698-1-AP), anti-Trap1 (10325-1-AP), anti-Actin(HRP-60008), anti-VDAC (10866-1-AP) (Proteintech, Chicago, IL, USA), anti-YB-1(sc-101198), anti-ATF5(sc-377168), anti-p-Akt(Ser473) (sc-101629)(Santa Cruz, CA, USA), p- eIF2α(Ser51)(3398), p-4EBP1(Thr37/46)(2855), p-Akt(Thr308) (13038), p-P70S6K(9204) (Cell Signaling Technology, USA).
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