The open field consists of a closed wooden box (72 × 72 cm). The walls and the floor are painted black and placed under dim red light (<10 lux). Rats were placed in the open field arena for testing. The arena was thoroughly cleaned between each trial with ethanol 10%. The rats’ movements were recorded and analyzed for 5 min using a video tracking system (Ethovision × T 14.0, Noldus Information Technology, Wageningen, The Netherlands) to measure anxiety-like behavior. The time spent in the arena center and the time spent freezing were calculated.
Ethovision t 14
Manufactured by Noldus
Ethovision × T 14.0 is a video tracking system designed for behavioral research. It is capable of automatically tracking and analyzing the movement and behavior of animals in a controlled environment. The system uses advanced computer vision algorithms to detect and follow the position and movement of animals in real-time.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ethovision t 14
1
Anxiety Assessment in Open Field Arena
2
Rat Behavior in Elevated Plus-Maze
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The apparatus consists of black plus-shaped maze (110 × 110 cm, 70 cm above the floor; two opposing open arms/closed arms). The rat was placed in the center of the maze and allowed to explore the maze freely for 5 min. The movements of the rat were recorded and analyzed using a video tracking system (Ethovision × T 14.0, Noldus Information Technology) to measure locomotion (measured as distance moved in cm). Freezing (s) and time spent in the closed arms (s) were used as a measure of anxiety-like behavior.
3
Rodent Anxiety Behavior Assessment
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The apparatus consists of a square black open field (50 × 50 × 50 cm3). The floor is divided by 1-cm-wide white lines into 25 squares measuring 10 × 10 cm2 each. The open field arena was thoroughly cleaned between each trial. The movements of the rat were recorded and analyzed for 30 min using a video tracking system (Ethovision ×T 14.0, Noldus Information Technology) to measure locomotion (measured as distance moved in cm). Freezing time (s) and time spent in the center (s) in the first 5 min were used as a measure of anxiety-like behavior [33 (link),46 (link)].
4
Open Field Test for Rat Anxiety
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The apparatus consists of a square black open field (50 × 50 × 50 cm). The floor is divided by 1 cm wide white lines into 25 squares measuring 10 × 10 cm each. The open field arena was thoroughly cleaned between each trial. The movements of the rat were recorded and analyzed for 15 min using a video tracking system (Ethovision × T 14.0, Noldus Information Technology) to measure locomotion (measured as distance moved in cm). Time spent in the center (s) in the first 5 min was used as a measure of anxiety-like behavior (Bauminger et al., 2022 (link)).
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