Antibodies used include anti-CD3 (clone OKT3), anti-CD28 (clone CD28.2, Cat# 555725, BD Pharmingen), anti-CD3-FITC (clone UCHT1, Cat# 21620033, Immunotools), anti-CD14-APC (clone MEM-15, Cat# 21279146, Immunotools), anti-IFN-γ PerCP Cy-5.5 (Clone B27, Cat # 560704, BD biosciences), rabbit polyclonal IgG (C-20) anti-human NF-κB primary antibody (SC-372R, Santa Cruz Biotechnology), Alexa Fluor 594, goat anti-rabbit IgG (H + L) secondary antibody (Cat# R37117, Molecular Probes, Invitrogen). Fluorescent reagents include LIVE/ DEAD Fixable Near-IR (Cat# L34976, Molecular Probes, Invitrogen), and DAPI nuclear stain (Cat# 00–4959-52, Thermo Fisher) and CellTrace Violet (CTV) (Cat# C34557, Molecular probes, Invitrogen). RPMI 1640 (Gibco, Life Technologies) complete medium supplemented with 10% fetal calf serum (FCS), 100 units/ml penicillin, 100 μg/ml streptomycin (all from GIBCOBRL®, Life Technologies™), 1 M HEPES buffer solution (Gibco, Life Technologies), 100 mM Sodium-pyruvate (Gibco, Life Technologies), MEM NEAA (100x) (Gibco, Life Technologies), 2-Mercaptoethanol (Sigma), Mycobacterial antigens used include: PPD for in vitro use (Statens Serum Institute, Copenhagen, Denmark) and ds-Red fluorescent protein expressing BCG (strain Myc3305, provided by Dr. Brigitte Gicquel) (Abadie et al., 2005 (link)).
Anti cd3 fitc clone ucht1
Manufactured by Immunotools
Anti-CD3-FITC (clone UCHT1) is a monoclonal antibody labeled with the fluorescent dye FITC. It is designed to bind to the CD3 receptor expressed on the surface of T cells.
Automatically generated - may contain errors
Lab products found in correlation
Celltrace violet, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hepes buffer solution, by Thermo Fisher Scientific (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Live dead fixable near ir, by Thermo Fisher Scientific (1 mentions)
Mem neaa 100x, by Thermo Fisher Scientific (1 mentions)
Dapi nuclear stain, by Thermo Fisher Scientific (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
2 protocols using anti cd3 fitc clone ucht1
1
Flow Cytometry Analysis of T-cell Activation
2
Multi-Marker Immunophenotyping of T Cells
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Anti-CD3-FITC (Clone UCHT-1), anti-CD4-APC (Clone MEM-241), anti-CD8-APC (Clone UCHT-4), and anti-CD62L-APC (Clone LT-TD180) were purchased from Immunotools. Anti-CD45RA-PE-cy7 (Clone HI100) was purchased from Invitrogen (Carlsbad, CA, USA). Freshly isolated PBMCs (1×106 cells) and the transduced T cells (1×105 cells) were incubated with specific antibodies (1:100) at 4°C for 45 minutes, and then examined by FACSVerse™ flow cytometer (BD Biosciences). The acquired data was analyzed using FlowJo software.
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