Activated partial thromboplastin time reagent

Manufactured by Diagnostica Stago

Sourced in France

The Activated partial thromboplastin time (aPTT) reagent is a laboratory product used to measure the clotting time of blood. It is used to assess the function of the intrinsic and common coagulation pathways. The reagent contains phospholipids and an activator that triggers the coagulation process, allowing for the evaluation of the clotting time.

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Lab products found in correlation

Fxiia, by Enzyme Research (3 mentions) Kaolin, by Thermo Fisher Scientific (2 mentions) Hrp conjugated antihemagglutinin ha igg, by Thermo Fisher Scientific (2 mentions) S2302 h d prolyl l phenylalanyl l arginine p nitroanilide, by Diapharma (2 mentions) S 2366 l pyro glu l pro l arg p nitroanilide, by Diapharma (2 mentions) Soybean trypsin inhibitor sbti, by Merck Group (2 mentions) Streptavidin hrp, by Thermo Fisher Scientific (2 mentions) Corn trypsin inhibitor, by Enzyme Research (2 mentions) Horseradish peroxidase conjugated hrp goat antihuman fxii, by Enzyme Research (2 mentions)

3 protocols using activated partial thromboplastin time reagent

Aptamer Selection for Factor XI Inhibition

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The aptamer library comprised a ssDNA template with sequence 5′-GAATTCTAAT ACGACTCACT ATA-N₄₀-GCGTCCAACA CATCG-3′ (please note spaces have been introduced every 10 nucleotides, into this and all other DNA sequences reported here). The forward (A) and reverse primers (B) were 5′-GAATTCTAAT ACGACTCACT ATA-3′ and 5′-GCGTCCAACAC ATCG-3′ respectively. These and all other oligonucleotides employed in this study were purchased from Integrated DNA Technologies (IDT, Coralville, IA). FXI, FXIa, FIX and FXIIa were bought from Enzyme Research Laboratories (South Bend, IN). Biotinylated goat anti-human Factor XI (FXI) antibody was purchased from Affinity Biologicals (Ancaster, ON). Dynabeads Biotin Binder was bought from Thermo Fisher Scientific (Waltham, MA). For thrombin generation assays (TGA), TGA substrate and TGA calibrator sets were obtained from Technothrombin GmbH (Vienna, Austria). Activated Partial Thromboplastin Time (APTT) reagent was from Diagnostica Stago (Asnieres, France). Chromogenic substrate S2366 was purchased from Instrumentation Laboratory (Lexington, MA).

Donkor D.A., Bhakta V., Eltringham-Smith L.J., Stafford A.R., Weitz J.I, & Sheffield W.P. (2017). Selection and characterization of a DNA aptamer inhibiting coagulation factor XIa. Scientific Reports, 7, 2102.

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Assay of Contact Activation Factors

Cited 1 time

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Human pooled normal plasma (Precision BioLogic). FXII-deficient plasma (George King Biomed). FXII, FXIIa, βFXIIa, PK, PKa, XI, XIa, corn-trypsin inhibitor (CTI), and horseradish peroxidase-conjugated (HRP) goat antihuman FXII (Enzyme Research Laboratory). Soybean trypsin inhibitor (SBTI) (Sigma-Aldrich). S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) and S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide (DiaPharma). Activated partial thromboplastin time (aPTT) reagent (Diagnostica Stago). Kaolin (ACROS Organics). Phe-Pro-Arg-chloromethylketone (FPRCK) (Prolytix). Long-chain PolyP with a broad range of polymer lengths (200-1300 orthophosphate units), and size-fractionated short-chain PolyP (PolyP-31, 27-35 units) have been described [12 (link)]. HRP-conjugated antihemagglutinin (HA) IgG (Invitrogen). Monoclonal biotinylated anti-FXI IgG 14E11 has been described [13 (link)]. Streptavidin-HRP (Thermo Scientific).

Hara-Kudo Y., Ikedo M., Kodaka H., Nakagawa H., Goto K., Masuda T., Konuma H., Kojima T, & Kumagai S. (2000). Selective Enrichment with a Resuscitation Step for Isolation of Freeze-Injured Escherichia coli O157:H7 from Foods. Applied and Environmental Microbiology, 66(7), 2866-2872.

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Intrinsic Coagulation Pathway Analysis

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Human pooled normal plasma (Precision BioLogic). FXII-deficient plasma (George King Biomed). FXII, FXIIa, βFXIIa, PK, PKa, XI, XIa, corn-trypsin inhibitor (CTI), and horseradish peroxidase-conjugated (HRP) goat antihuman FXII (Enzyme Research Laboratory). Soybean trypsin inhibitor (SBTI) (Sigma-Aldrich). S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) and S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide (DiaPharma). Activated partial thromboplastin time (aPTT) reagent (Diagnostica Stago). Kaolin (ACROS Organics). Phe-Pro-Arg-chloromethylketone (FPRCK) (Prolytix). Long-chain PolyP with a broad range of polymer lengths (200-1300 orthophosphate units), and size-fractionated short-chain PolyP (PolyP-31, 27-35 units) have been described [12] . HRP-conjugated antihemagglutinin (HA) IgG (Invitrogen). Monoclonal biotinylated anti-FXI IgG 14E11 has been described [13] . Streptavidin-HRP (Thermo Scientific).

Shamanaev A., Litvak M., Cheng Q., Ponczek M., Dickeson S.K., Smith S.A., Morrissey J.H, & Gailani D. (2023). A site on factor XII required for productive interactions with polyphosphate. Journal of thrombosis and haemostasis : JTH, 21(6).

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