Intravenous catheter surgeries were performed to allow drug self-administration. Catheters were assembled from a cannula connector pedestal (Plastics One Inc., Minneapolis, MN, USA) connected to a 95 mm silicone catheter (0.3 mm inner diameter × 0.6 mm outer diameter) and a 6 mm protective sleeve of polyethylene tubing (0.75 mm inner diameter × 1.45 mm outer diameter). Following arrival, rats were habituated to the animal facility for 1 week. The surgical procedure was executed as reported previously (De Vries et al., 1999 (link)) under isoflurane gas anesthesia. Thirty minutes before surgery, rats were injected with the analgesic Ketofen (5 mg/kg; Merial, Velserbroek, The Netherlands) and the antibiotic Baytril (8.33 mg/kg; Bayer, Mijdrecht, The Netherlands). The local anesthetic 2% Xylocaine with adrenaline (10 mg/kg; Astra Zeneca, Zoetermeer, The Netherlands) was injected in the scalp, after which the skull was exposed and four burr holes were drilled and fitted with jeweler’s screws. Catheter tubing was tunneled from the scalp to an incision above the clavicle, where the catheter was inserted in the jugular vein and fixed in place using sterile sutures. A combination of 0.05 ml taurolidine-citrate solution (TCS; Access Technologies, Skokie, IL, USA) and a polyethylene cap was used to maintain catheter patency during the 1 week minimum period of recovery.
Xylocaine with adrenaline
Manufactured by AstraZeneca
Sourced in United Kingdom
Xylocaine with adrenaline is a local anesthetic solution containing lidocaine hydrochloride and epinephrine. It is used to provide local or regional anesthesia.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using xylocaine with adrenaline
1
Intravenous Catheter Implantation for Drug Self-Administration
2
Vastus Lateralis Muscle Biopsy Protocol
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The biopsy procedure was conducted under local anesthesia (Xylocaine with adrenaline, 10 mg/mL lidocaine +5 μg/mL adrenaline, AstraZeneca, London, UK) and muscle tissue was obtained using a modified Bergström technique with suction. The tissue was quickly rinsed in physiological saline, before fat, connective tissue, and blood were removed and discarded. Subsequently, the samples were quickly frozen in isopentane and cooled on dry ice or liquid nitrogen, before being transferred and stored at -80°C for later isolation of DNA and RNA. All biopsies from both pre and post training time points were taken at rest, in the morning after an overnight fast from the lateral portion of vastus lateralis muscle. All participants were asked only to consume water during the overnight fast and therefore did not consume alcohol and caffeine the night before or the morning of the pre and post aerobic training biopsy. The participants were also asked not to perform any exercise 2 days before the pre training biopsy. Post training samples were obtained at least 72 hrs after the end of the training intervention. Pre and post biopsies were taken from the left leg in all participants.
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