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Anti pplcγ

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Anti-pPLCγ is a lab equipment product from Cell Signaling Technology that detects the phosphorylated form of phospholipase C gamma (PLCγ). PLCγ is an important signaling enzyme involved in various cellular processes.

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Lab products found in correlation

Anti p erk, by Cell Signaling Technology (3 mentions) Anti akt, by Cell Signaling Technology (2 mentions) Anti p akt, by Cell Signaling Technology (2 mentions) Anti plcγ, by Cell Signaling Technology (2 mentions) Anti human cd3, by BD (1 mentions) Anti human cd8, by BD (1 mentions) Anti rna polymerase 2, by Abcam (1 mentions) Anti mouse cd4, by Thermo Fisher Scientific (1 mentions) Anti zap70, by Cell Signaling Technology (1 mentions) Anti mouse cd8, by BD (1 mentions) Anti mouse cd25, by BD (1 mentions) Anti mouse tcrβ, by BD (1 mentions) Anti mouse cd4, by BD (1 mentions) Anti mouse ifn γ, by BioLegend (1 mentions) Anti human cd11a, by BioLegend (1 mentions) Anti mouse cd69, by Thermo Fisher Scientific (1 mentions) Anti mouse il 2, by Thermo Fisher Scientific (1 mentions) Anti dbf4, by Abcam (1 mentions) Anti rna polymerase 2 phospho s2, by Abcam (1 mentions) Anti gapdh, by BioLegend (1 mentions)

4 protocols using anti pplcγ

1

Detailed Immunological Antibody Protocols

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The antibodies used in this study for flow cytometry include: anti-mouse CD8 (#563786), anti-mouse CD25 (#558642), anti-mouse TCRβ (#553174), anti-human CD3 (#563798), anti-human CD8 (#563795), and anti-human TCRαβ (#563826) from BD Biosciences; anti-mouse CD4 (#100544), anti-mouse IFN-γ (#505835), anti-human LFA-1 (#363404), anti-human CD11a (#301208), anti-human CD18 (#302114), anti-human CD25 (#302625), and anti-human CD69 (#310920) from BioLegend; anti-mouse CD4 (#17-0042-83), anti-mouse CD4 (#17-0041-83), anti-mouse CD69 (#25-0691-82), anti-mouse TNF (#17-7321-82), anti-mouse IL-2 (#12-7021-82), anti-human CD4 (#17-0048-2), and anti-human CD8 (#11-0088-41) from eBioscience.
The antibodies used for immunoprecipitation and immunoblotting include: anti-Cdc7 (#ab10535), anti-Dbf4 (#ab124707), anti-RNA polymerase II (#ab817) and anti-RNA polymerase II (phospho S2, # ab193468) from Abcam; anti-PLCγ (#610027) and anti-ERK (#610031) from BD Biosciences; anti-LAT (#641102) from BioLegend; anti-GAPDH (#2118), anti-pPLCγ (#2821), anti-ZAP70 (#2709), anti-pZAP70 (#2717), anti-pERK (#4370), anti-pLAT (#3584), anti-pLck (#6943), and anti-NF-κB (#3035) from Cell Signaling; anti pMCM2 S40 (#GTX62847) from GeneTex; anti-MCM2 (#MA5-15895) from Pierce; and anti-Lck (#sc-433) from Santa Cruz.
Chen E.W., Tay N.Q., Brzostek J., Gascoigne N.R, & Rybakin V. (2019). A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Frontiers in Immunology, 10, 1718.
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2

Comprehensive Protein Expression Analysis

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The assays were performed as previously described 13 (link), using anti-Cdc42, anti-P21, anti-vWF, anti- VEGFC (Proteintech), anti-β-actin, anti-E-cadherin, anti-SIX1 anti-cyclinD1(Santa Cruz Biotechnology), anti-ERK, anti-p-ERK, anti-AKT, anti-p-AKT, anti- PLC-γ, anti-p-PLC-γ, anti-STAT3, anti-p-STAT3, anti- EGFR, anti-MMP-9, anti-MMP-11, and anti-PCNA (Cell Signaling Technology) antibodies.
Zhao Z., Li L., Du P., Ma L., Zhang W., Zheng L., Lan B., Zhang B., Ma F., Xu B., Zhan Q, & Song Y. (2019). Transcriptional Downregulation of miR-4306 serves as a New Therapeutic Target for Triple Negative Breast Cancer. Theranostics, 9(5), 1401-1416.
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3

PD-1-PD-L1 Signaling Pathway Analysis

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DC-1 cells were prepulsed with 5 μM MCC88-103 overnight at 37 °C and washed before the assay. 2 × 106 mPD-1-deleted 2D12 cells transduced with hPD-1 were stimulated with 2 × 106 DC-1 cells not transduced or transduced with hPD-L1. The cells were lysed with the lysis buffer (50 mM Tris-HCl, 50 mM NaCl, and 5 mM EDTA) containing 1% NP-40. Whole cell lysates (WCLs) or those immunoprecipitated by anti-GFP (MBL International, D153-11, RRID:AB_2893312) were blotted with anti-GFP (1:5000, Miltenyi Biotec, 130-091-833, RRID:AB_247003), anti-mSHP1 (1:500, Santa Cruz Biotechnology, sc-287, RRID:AB_2173829), anti-mSHP2 (1:1000, Santa Cruz Biotechnology Inc., sc-7384, RRID:AB_628252), anti-PLCγ (1:1000, Cell Signaling Technology, 5690, RRID:AB_10691383), anti-pPLCγ (1:1000, Cell Signaling Technology, 8713, RRID:AB_10890863), anti-Akt (1:2000, Cell Signaling Technology, 4691, RRID:AB_915783), anti-pAkt (1:1000, Cell Signaling Technology, 4060, RRID:AB_2315049), anti-Erk (1:1000, Cell Signaling Technology, 4695, RRID:AB_390779), or anti-pErk (1:1000, Cell Signaling Technology, 4370, RRID:AB_2315112) as a first antibody and HRP-anti-rabbit IgG polyclonal Abs (1:10,000, Cell Signaling Technology, 7074, RRID:AB_2099233) as a second one. Each intensity of band was calculated by ImageJ (RRID:SCR_003070).
Nishi W., Wakamatsu E., Machiyama H., Matsushima R., Saito K., Yoshida Y., Nishikawa T., Takehara T., Toyota H., Furuhata M., Nishijima H., Takeuchi A., Azuma M., Suzuki M, & Yokosuka T. (2023). Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters. Nature Communications, 14, 3157.
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4

Investigating MAPK, Akt, and PLCγ Signaling in ALS Mice

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A single intraperitoneal injection of 0.5 mg/kg 2B7 (~10 μg) was done in wild type C57-BL6 mice (n = 3 per group) or ALS mice (n = 5 per group), and tissue was collected ~18 hours post-injection of 2B7 or control IgG. Detergent lysates were analyzed by Western blotting with Anti-phospho-MAPK, anti-phospho-Akt, or anti-p-PLCγ (all from Cell Signaling). After stripping, membranes were re-probed with anti-actin (Sigma) to standardize loading. Quantification was done by densitometric analysis.
Brahimi F., Maira M., Barcelona P.F., Galan A., Aboulkassim T., Teske K., Rogers M.L., Bertram L., Wang J., Yousefi M., Rush R., Fabian M., Cashman N, & Saragovi H.U. (2016). The Paradoxical Signals of Two TrkC Receptor Isoforms Supports a Rationale for Novel Therapeutic Strategies in ALS. PLoS ONE, 11(10), e0162307.
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