Dye reagent for protein titration

Goat anti-Rabbit-Horseradish peroxidase-conjugated IgGs (GAR-HRP) and Goat anti-Mouse-Horseradish peroxidase-conjugated IgGs (GAM-HRP) were purchased from Immunoreagent (Raleigh, NC, USA). Fuji Super RX 100 films (Laboratorio Elettronico Di Precisione, Naples, Italy), polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Milan, Italy), and dye reagent for protein titration (Bio-Rad, Hercules, CA, USA) were used for Western blotting. Salts, buffers, and Bovine serum albumin fraction V (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Bovine serum albumin fraction V (BSA), rabbit anti-human haptoglobin, salts, and buffers were purchased from Sigma-Aldrich (St. Louis, MO, USA). The dye reagent for protein titration was from Bio-Rad (Hercules, CA, USA), and the polyvinylidene difluoride (PVDF) membrane was from GE Healthcare (Milan, Italy). Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Immunoreagent, (Raleigh, NC, USA) (goat anti-rabbit or goat anti-mouse) or from Sigma-Aldrich (St. Louis, MO, USA) (rabbit anti-goat). Fuji Super RX 100 films were from Laboratorio Elettronico Di Precisione (Naples, Italy).

Salts, buffers and Bovine serum albumin fraction V (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Colorimetric enzymatic kits were purchased from Sigma Aldrich (St. Louis, MO, USA) for fructose, GS Diagnostics SRL (Guidonia Montecelio, Rome, Italy) for glucose and uric acid and SGM Italia (Rome, Italy) for triglycerides. Elisa kit for insulin determination was purchased from Mercodia AB (Uppsala, Sweden). Chemiluminescent substrate, Immobilon (Millipore Corporation, Billerica, MA 01821, USA), Excellent Chemiluminescent detection Kit (ElabScience, Microtech, Naples, Italy), Polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA) and dye reagent for protein titration (Bio-Rad, Hercules, CA, USA) were used for Western blotting. The antibodies used for Western blot analysis were purchased by Cell Signaling (Danvers, MA, USA) for p-Akt and Akt1, Santa Cruz Biotechnology (Santa Cruz, CA, USA) for p-GSK, GSK-3β, GLUT-4 and VDAC1, Invitrogen (Carlsbad, CA, USA) for GLUT-5, Calbiochem (San Diego, CA, USA) for UCP-3, Abcam, (Cambridge, UK) for Oxphos, Biogenesis Ltd. (England, UK) for ANT, Sigma-Aldrich (St Louis, MO, USA) for actin.