Lipids were extracted from 100 mg liver tissue with 1 mL chloroform. Subsequently, the liver was desiccated using a micro-size centrifugal concentrator spin-dryer mini (TAITEC, Saitama, Japan), and was then weighed. The desiccated liver tissues were dissolved into concentrated nitric acid at 20 mg/mL. The solution was diluted with iron-free deionized water to bring the final nitric acid concentration to 0.1 mM. Non-heme iron was measured as hepatic iron content using an atomic absorption spectrophotometer (Hitachi, Tokyo, Japan) and expressed as μg non-heme iron/g dry liver tissue weight (μg Fe/g dry wt).
Atomic absorption spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The Atomic Absorption Spectrophotometer is a laboratory instrument used for the quantitative determination of chemical elements using the principles of atomic absorption. It measures the concentrations of elements in a sample by absorbing light at specific wavelengths.
Automatically generated - may contain errors
Lab products found in correlation
12 protocols using atomic absorption spectrophotometer
1
Hepatic Iron Content Analysis
2
Mineral Composition Analysis of Leaf Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We dried the leaves in an oven for 24 h at 70 °C. We digested ground leaves with HNO3 and HClO4 to determine the mineral elements [62 (link),63 (link)]. We digested a 0.5 g leaf sample with 400 mL HNO3 (65%), 40 mL HClO4 (70%), and 10 mL H2SO4 (96%). We read the absorbance at 589 (Na), 213.9 (Zn), 258.056 (S), 285.2 (Mg), 248.3 (Fe), 76 6.5 (K), 422.7 (Ca), 279.5 (Mn), 880 (P), 324.8 (Cu), 313.3 (Mo), and 430 (B) nm wavelengths using a Hitachi atomic absorption spectrophotometer (Tokyo, Japan). We expressed macro- and microelements in mg g−1 and µg g−1 DW.
3
Cr(VI) Quantification in Microbial Fuel Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Na2Cr2O7 of special grade chemicals was dried at 200 °C for 1 h and left in a desiccator. Subsequently, 25.2 mg of Na2Cr2O7 was weighed and dissolved in water and diluted to 100 mL. The diluted solution was used as a standard stock solution of 100 mg/L of Cr (VI). The Cr(VI) was first chelated with ammonium pyrrolidine dithiocarbamate (APDC) and then extracted with methyl isobutyl ketone (MIBK) [30 ]. The extract was aspirated into the flame of the atomic absorption spectrophotometer (Hitachi, Tokyo, Japan). The colorimetric method for Cr(VI) measurement was performed as described previously [31 (link)].
The MFC potential or voltage was measured using a multimeter (Model 2700, Keithley Instruments, Inc., Solon, OH, USA). Data were digitally recorded every minute on a computer by using an interface card (Model PCI-488, Keithley Instruments, Inc.). The measured voltage (V) was converted to current (I) according to the following relationship: voltage (V, volt) = current (I, amp) × resistance (R, ohm). The power (P, watt) was calculated as P = I × V and then normalized using the surface area of the anode. All experiments were conducted using five separate MFCs, and all analyses were conducted at least in triplicate.
The MFC potential or voltage was measured using a multimeter (Model 2700, Keithley Instruments, Inc., Solon, OH, USA). Data were digitally recorded every minute on a computer by using an interface card (Model PCI-488, Keithley Instruments, Inc.). The measured voltage (V) was converted to current (I) according to the following relationship: voltage (V, volt) = current (I, amp) × resistance (R, ohm). The power (P, watt) was calculated as P = I × V and then normalized using the surface area of the anode. All experiments were conducted using five separate MFCs, and all analyses were conducted at least in triplicate.
4
Serum Chromium Analysis in Dams and Offspring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The blood samples from the dams at weaning and the 16-week-old offspring were centrifuged and the resulting serum was collected for Cr analysis. Serum Cr concentrations were measured using an atomic absorption spectrometer (Atomic Absorption Spectrophotometer; Hitachi, Ltd., Tokyo, Japan).
5
Cellular Na+ and K+ Concentrations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Inocula were prepared as described in the section on salt resistance assays. Each inoculum (500 μl, OD600 ≈0.2) was smeared onto TY agar plates containing different salts (no added salt, 0.4 M NaCl or 0.3 M KCl) and incubated at 28°C for 4 days (plates containing 0 and 0.4 M NaCl) or 7 days (plates containing 0.3 M KCl). The bacteria were then collected in 1.5-ml sterile tubes using sterile stainless steel spoons and dried at 60°C for 10 h using an air dryer. The total cellular Na+ and K+ concentrations were measured using an atomic absorption spectrophotometer (Hitachi, Tokyo, Japan).
6
Serum Chromium Analysis at Weaning and 8 Months
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum was obtained from the dams at weaning and from the offspring at 8 months of age for chromium analysis. The serum chromium concentration was measured by atomic absorption spectrometer (Atomic Absorption Spectrophotometer, Hitachi, Japan).
7
Measurement of K and Na in Amaranthus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dried leaf powder of A. tricolor was analyzed for measurement of K and Na content following the nitric-perchloric acid digestion method according to Sarker and Oba (2018a) (link) at the wavelength of 766.5 nm (K) and 589.0 nm (Na) using Hitachi atomic absorption spectrophotometer (AAS) (Japan).
8
Maternal and Offspring Serum Chromium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum was obtained from the dams (n = 8 per group) at weaning and offspring (n = 8 per group) at eight months of age for chromium analysis. The blood samples were collected in serum-separated tubes to obtain serum. The coagulated blood was left to clot at room temperature for 30 min and then centrifuged for 15 min at 2000 rpm at 4 °C. The supernatant fluid was then separated and stored frozen (−80 °C) for analysis. For chromium determination, serum was diluted at a ratio of 1:4 in 0.1% Triton-X 100 and 0.01 mol/L nitric acid. All laboratory glassware was soaked by nitric acid and rinsed with deionized water to avoid metal contamination. The serum chromium concentration was measured by an atomic absorption spectrometer (Atomic Absorption Spectrophotometer, Hitachi, Japan). Chromium standard solution for the calibration curve (0, 0.1, 0.2, 0.5, 1, 2 ng/mL Cr) was freshly prepared by serial dilution of the stock solution (100 ng/mL Cr) with 0.01 mol/L nitric acid.
9
Cellular Na+ Quantification Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Inocula were prepared as described in the section on the salt and alkali resistance assay. Five hundred microliters of each inoculum (OD600 ≈ 0.2) were plated on TY agar plates with and without 0.4 M NaCl and inoculated at 28 °C for 7 days. The bacteria were collected in 1.5-ml sterile tubes and dried using an air dryer at 50 °C for 12 h. The total cellular Na+ content was measured using an atomic absorption spectrophotometer (Hitachi, Tokyo, Japan).
10
Caco-2 Cell Calcium Transport Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Caco-2 cells were purchased from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences (Shanghai, China). The formula of minimal essential medium (MEM) is 1% penicillin-streptomycin-neomycin antibiotic mixture and 20% fetal bovine serum. The Caco-2 cell monolayer model was established and studied according to the methods of previous studies (24 (link), 25 (link)), the resistance value of the monolayer in this calcium transport experiment is >500 Ω/cm2. 0.5 mM peptide LGKDQVRT and 5 mM CaCl2 or 1 mM peptide LGKDQVRT and 5 mM CaCl2 were dissolved in 0.5 mL of Hank's balanced salt solution (HBSS) pH 7.4 and added to the upper layer, respectively. Cells are cultured in the medium, and then incubated in a constant temperature and humidity incubator with a temperature of 37°C and a humidity of 5% CO2 for 2 h.
At 30, 60, 90, and 120 min, draw sample to be tested from the basolateral side, and add the same volume of fresh HBSS buffer to keep the volume constant. Use atomic absorption spectrophotometer (Hitachi, Tokyo, Japan) to determine the calcium content in the sample to be tested.
At 30, 60, 90, and 120 min, draw sample to be tested from the basolateral side, and add the same volume of fresh HBSS buffer to keep the volume constant. Use atomic absorption spectrophotometer (Hitachi, Tokyo, Japan) to determine the calcium content in the sample to be tested.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!