A 1 mL plastic syringe (BD LUER-LOK) filled with DPhPC liposomes (2–5 mg mL−1) was attached to a ~2 cm long MICROFIL needle (CMF90UxxL, 36 gauge, 20 μm inner diameter, 90 μm outer diameter; World Precision Instruments). The needle was inserted into the encapsulated oil drop by piercing the solidified hydrogel encasing (22 ± 1 °C), and was held on the upper edge of the oil drop taking care that the needle end did not touch the hydrogel. By a slight push of the piston, the liposome solution was expelled to form a small aqueous droplet (100–400 μm in diameter). A quick withdrawal of the MICROFIL needle from the oil allowed the aqueous droplet to separate from the needle and slowly fall to the bottom of the oil drop. The small holes created in the hydrogel by piercing with the needle became resealed. Keeping the hydrogel in oil also prevented the oil oozing out of the hydrogel by maintaining a flow equilibrium while the holes became resealed or if the holes did not properly reseal.
Luer lok
Manufactured by BD
Sourced in United States
The BD Luer Lok is a device used to securely connect medical equipment, such as syringes, IV tubing, and other lab instruments. It features a tapered locking mechanism that allows for a tight, leak-proof connection. The BD Luer Lok is designed to meet industry standards for safety and reliability.
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Lab products found in correlation
11 protocols using luer lok
1
Encapsulated Liposome Droplet Formation
2
Fluidic Device Outlet Connections
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Inlets of all fluidic devices were designed to allow pipetting reagents into inlet wells. Outlets were designed to press-fit with a Tygon Microbore tube (0.060” outer diameter). The press-fit outlet-tube connection was checked for leaks. In the case of a leaky fit, parafilm was used to create an airtight fit. The outlet tube (Tygon Microbore, 0.060” OD, 10–15 cm long) was connected to a 23-gauge, ½” dispensing needle tip & syringe (1 mL BD Luer Lok) and loaded into the syringe pump.
3
Culturing 4T1 Cells in Hollow Fiber Scaffold
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Before loading cells, the channels of the HFSM were pre-incubated with the cell culture media for 30 min. 4T1 cells were prepared in a T-75 flask and harvested in cell culture media at a concentration of 500,000 cells/mL. A cell solution of 100 μL was injected into the culture chamber of the HFSM through the center channel using a 1 mL syringe (BD Luer-Lok™, sterile) and 0.02 inch x 0.06 inch (I.D. × O.D.) tubing (Tygon® ND-100-80, autoclaved before usage), and then the HFSM was placed in the cell culture incubator. Cell culture media (RPMI-1640 containing 10% FBS and 1% PS) in the HFSM was changed every 12 hr.
4
Generating Microdroplets for Cell Encapsulation
5
Density Determination of Corrugated Microparticles
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A pycnometer (Ultrapyc 1200e, Quantachrome, Boynton Beach, FL, USA) was used to determine the true density of the corrugated microparticles under helium gas. The temperature was maintained at 20–21 °C, and each sample was run five times. Tapped density of corrugated microparticles was measured using 1 cc Luer-Lock syringe with a cap (BD Luer-Lok™, BD bioscience, Franklin Lakes, NJ, USA) containing a linear scale with 0.1 ml increments. The syringe with 100 mg the powder sample was then tapped manually on a flat workbench until the volume remained constant, and the volume was noted as the tapped volume.
6
Isolation of Salmonella Phages from Environmental Sources
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A total of 60 samples were used for isolation of Salmonella phages, including, sewage (primary sludge, Guelph, Fergus, Hamilton, and Waterloo, wastewater treatment plants, Ontario, Canada), retail duck and chicken skin, chicken and geese feces, chicken and turkey feathers, run-off water from poultry processing plants, and river water (Speed River, Guelph, Ontario, Canada). Samples were processed differently according to their physical nature. For solid samples (e.g. chicken skin) ~10 g of sample was mixed with 10 ml of 2X TSB, the sample was immediately enriched as described in section 2.3. Semisolid samples (e.g. primary sludge) were processed as previously described (Yu et al. 2016 ). Briefly, primary sludge was incubated with 50 mM tetrasodium pyrophosphate (Na4P2O7, Thermo Fisher Scientific™) at room temperature with moderate agitation and centrifuged at 10 000 × g for 10 min. Supernatant was then filtered using 0.45-µm syringe filter (BD Luer-Lok™). A volume of 10 ml of the filtrate was mixed with 10 ml of 2X TSB and the sample was enriched as described in section 2.3. Liquid samples (e.g. river water) were centrifuged at 10 000 × g for 10 min and filtered using a 0.45-µm syringe filter. A total of 10 ml of the filtrate were mixed with 10 ml of 2X TSB, samples were then enriched as described below.
7
Fat Tissue Harvesting and Purification
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Under local anesthesia, fat tissue was harvested from the lateral thigh of a 25-year-old healthy woman using a 2 mm diameter blunt cannula attached to a 10 mL Luer-Lok syringe (Becton Dickinson Co., NJ, USA) under manual regulation of negative pressure. The aspirated fat was washed with 0.9% sodium chloride solution (saline), held in the syringe vertically for 3 minutes, and then separated into three layers (the upper layer was composed of oil, the middle layer was composed of adipose tissue, and the lower layer was composed of blood). The upper and lower layers were discarded. The procedure above was repeated three times to obtain 60 mL of purified fat. The participant provided informed consent, and an institutional review board-approved protocol was used.
8
Xenograft Fat Grafting Protocol
9
Injection Capacity Evaluation with Syringes
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Injection capacity of all solutions were tested immediately after the synthesis, by using 10 mL disposable syringes (BD, Luer-Lok™, (Becton, Dickinson U.K. Limited, Wokingham, UK) with a series of needle sizes; 18G ×1 1/2” (1.2 × 40 mm), 19G ×1 1/2” (1 × 40 mm), 20G ×1 1/2” (0.8 × 40 mm), 21G ×1 1/2” (0.8 × 40 mm), 22G ×1 1/2” (0.7 × 40 mm), 23G ×1” (0.6 × 25 mm), and 25 G ×5/8” (0.5 × 16 mm). The injection observations for each solution were rated comparatively.
10
Optimized Cell Seeding for Bioreactors
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For all runs, 5.2 × 107 cells in 100 mL NSC media were loaded into a cell inlet bag using a 60-mL Luer-Lok syringe (BD Biosciences, San Jose, CA) in a biosafety cabinet. The number of cells loaded was 30% greater than the 4 × 107 cells we considered seeded (plating density 2 × 103 cells/cm2) to account for cell adhesion to the tubing surrounding the bioreactor in the IC loop. The cell bag was then welded to the cell inlet line using the TSCD – Q tubing welder (Terumo BCT).
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