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Jem 1400 lab6 tem

Manufactured by JEOL

The JEM-1400 LaB6 TEM is a transmission electron microscope manufactured by JEOL. It uses a lanthanum hexaboride (LaB6) electron source to generate an electron beam, which is then focused and directed onto the sample. The instrument allows for the observation and analysis of microscopic structures and features at high magnifications.

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2 protocols using jem 1400 lab6 tem

1

Nanomaterial Characterization by TEM

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Intact or digested NTFs (10 μM of peptide content) in buffer (20 μL) were transferred onto Formvar/carbon-coated 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). Excess NTFs were blotted off using filter paper and the samples were stained with 0.5% (v/v) uranyl formate solution (20 μL). After drying at room temperature, the grids were examined under TEM (JEOL JEM-1400 LaB6 TEM operating at 120 Kv).
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2

Transmission Electron Microscopy of Nanoparticles and Tissue

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NFP (10 × 10−6m, 20 μL) was dropped onto formvar/carbon-coated 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA) and an excess sample was removed with filter paper. After uranyl formate staining (0.5% v/v, 20 μL), the grids were examined under TEM (JEOL JEM-1400 LaB6 TEM operating at 120 kV). For the TEM analysis of the tumor sections, tissues were first fixed in modified Karmovsky’s fix solution (2.5% glutaraldehyde, 4% paraformaldehyde, and 0.02% picric acid in 0.1 m buffer) and then in reduced osmium tetroxide (1% OsO4− and 1.5% K-ferricyanide (aqueous). Following dehydration, the samples were embedded in an Epon analog resin (Embed812) and ultrathin sections (65 nm) were cut using a Diatome diamond-knife on Ultracut T ultramicrotone (Leica Microsystems, Wetzlar, Germany). The sections were contrasted with lead citrate and observed on the JEM 1400 electron microscope.
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