Jem 1400 lab6 tem

Intact or digested NTFs (10 μM of peptide content) in buffer (20 μL) were transferred onto Formvar/carbon-coated 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). Excess NTFs were blotted off using filter paper and the samples were stained with 0.5% (v/v) uranyl formate solution (20 μL). After drying at room temperature, the grids were examined under TEM (JEOL JEM-1400 LaB₆ TEM operating at 120 Kv).

NFP (10 × 10⁻⁶m, 20 μL) was dropped onto formvar/carbon-coated 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA) and an excess sample was removed with filter paper. After uranyl formate staining (0.5% v/v, 20 μL), the grids were examined under TEM (JEOL JEM-1400 LaB₆ TEM operating at 120 kV). For the TEM analysis of the tumor sections, tissues were first fixed in modified Karmovsky’s fix solution (2.5% glutaraldehyde, 4% paraformaldehyde, and 0.02% picric acid in 0.1 m buffer) and then in reduced osmium tetroxide (1% OsO⁴⁻ and 1.5% K-ferricyanide (aqueous). Following dehydration, the samples were embedded in an Epon analog resin (Embed812) and ultrathin sections (65 nm) were cut using a Diatome diamond-knife on Ultracut T ultramicrotone (Leica Microsystems, Wetzlar, Germany). The sections were contrasted with lead citrate and observed on the JEM 1400 electron microscope.