Myricetin

Manufactured by Fujifilm

Sourced in Japan

Myricetin is a flavonoid compound found in various plant sources, including berries, vegetables, and herbs. It is a natural antioxidant and has been studied for its potential biological activities. The core function of Myricetin is to serve as a research tool for investigating its chemical and biochemical properties.

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Lab products found in correlation

5 protocols using myricetin

Profiling Phenolics and Flavonoids in Edible Oils

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Fourteen edible oils (sunflower, safflower, canola, soybean, cotton, grape, flax, avocado, chia, Inca inchi, perillartine, sesame, and rice bran oil) were purchased from different supermarkets in Hiroshima Prefecture, Japan, in 2016. Details are described in Table 1. Standard chemicals for the analysis of individual phenolic acids (gallic acid, protocatechuic acid, catechol, chlorogenic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, syringic acid, vanillin, ferulic acid, sinapic acid, p-coumaric acid, benzoic acid, ellagic acid, and cinnamic acid), and flavonoids (esculetin, isoquercetin, myricetin, fisetin, morin, quercetin, luteolin, kaempferol, isohamnetin, apigenin, rhamnetin, and galangin), extracting solvents, and buffers were of analytical grade and purchased from Wako company, Tokyo, Japan.

Xuan T.D., Gangqiang G., Minh T.N., Quy T.N, & Khanh T.D. (2018). An Overview of Chemical Profiles, Antioxidant and Antimicrobial Activities of Commercial Vegetable Edible Oils Marketed in Japan. Foods, 7(2), 21.

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Polyphenol Compound Procurement for Biochemical Assays

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Flavone, 6-hydroxy flavone, 3-hydroxy flavone, 7-hydroxy flavone, 6-methoxy-flavone, 6-methoxy flavanone, naringenin and naringin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Biochanin A, genistein, apigenin, puerarin, wogonin, catechin (C), epicatechin (EC), luteolin, daidzein, daidzin, resveratrol, piceid and neohesperidin-dihydrochalcone were purchased from Aladin Co. Ltd. (Shanghai, China). Galangin, kaempferol, quercetin, myricetin, baicalein and chrysin were purchased from Wako Pure Chemical Industries (Osaka, Japan). All other polyphenols were obtained commercially from Shanghai Tauto Biotech CO., Ltd. (Shanghai, China). Peptone, yeast extract, agar, 1-deoxy-1-morpholino fructose (DMF) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nitrotetrazolium Blue chloride (NBT) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Kanamycin and carbenicillin were purchased from Shanghai Xinya Pharmaceutical Co., Ltd. (Shanghai, China). Fetal bovine serum (BP) of no phage and low endotoxin was from Sijiqing Biological Engineering Materials Co., Ltd. (Hangzhou, China). All other reagents and solvents were analytical grade and used without further purification.

Xie Y., Chen J., Xiao A, & Liu L. (2017). Antibacterial Activity of Polyphenols: Structure-Activity Relationship and Influence of Hyperglycemic Condition. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1913.

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Flavonoid Compounds Protocol

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Quercetin, fisetin, keamferol, and myricetin were purchased from Wako chemicals (Japan). Galangin and taxifolin were obtained from Sigma, morin from TCI (Japan), and luteolin from LKT laboratories (U.S.A.). Flavonoid stock solutions were prepared in dimethyl sulfoxide. All other chemicals were of analytical grade.

Widiatningrum T., Maeda S., Kataoka K, & Sakurai T. (2015). A pirin-like protein from Pseudomonas stutzeri and its quercetinase activity. Biochemistry and Biophysics Reports, 3, 144-149.

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Thioflavin-T Assay for Amyloid-β Aggregation

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The rate of Aβ aggregation was evaluated using a slightly modified thioflavin-T (Th-T) method described in a previous report [6 (link)]. The Th-T method was originally developed by Naiki and co-workers [48 (link)]. The rate of Aβ aggregation was calculated by comparing the fluorescence intensity of each sample with that of a control (25 μM of Aβ₄₀ and DMSO containing no test sample). The aggregation rate (%) was calculated using the following formula:

[(S - B) / (C - B) \times 100]

, (S, fluorescence of Th-T solution incubated with Aβ₄₀ and sample; C, fluorescence of Th-T solution incubated with Aβ₄₀ and DMSO; B, fluorescence of Th-T solution not incubated with Aβ₄₀ and DMSO). Myricetin (Fujifilm-Wako) was used as a positive control at 25 μM as the final concentration, and that of test samples (1–4,7–8) was 100 μM.

Fujihara K., Shimoyama T., Kawazu R., Sasaki H., Koyama K., Takahashi K, & Kinoshita K. (2020). Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus. Journal of Natural Medicines, 75(2), 284-298.

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Clonogenic Assay for Compound Screening

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HeLa or A-204 cells were plated 200 cells/well in a 6-well plate and then allowed to attach overnight. The next day, compounds were placed in the plates at various concentrations. In the case of multi-compound treatment, 1 mM L-NAME or 6 mM myricetin (Wako) was added into the plate, and then ALESIA was. The HeLa or A-204 cells were incubated for 14 days or 28 days respectively, after which they were fixed and stained with 0.25 % crystal violet (Nacalai Tesque) and 25 % methanol in PBS at RT (20-26 C) for 30 min. The wells were washed with water once and dried at RT. The wells were imaged using the Cell 3 iMager CC-5000 (SCREEN Holdings, Kyoto, Japan). All images were scanned with a resolution of 2400 dpi. The colonies over 400 mm in diameter were defined as survival and the number of them was calculated by using Cell3iMager software version 2.4 (SCREEN Holdings).

Toyomoto M., Inoue A., Iida K., Denawa M., Kii I., Ngako Kadji F.M., Kishi T., Im D., Shimamura T., Onogi H., Yoshida S., Iwata S., Aoki J., Hosoya T, & Hagiwara M. (2021). S1PR3-G₁₂-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation. Cell chemical biology, 28(8).

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