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GATA-6 is a protein that functions as a transcription factor, regulating the expression of genes involved in cell differentiation and development. It plays a key role in the regulation of diverse cellular processes. The GATA-6 product from Santa Cruz Biotechnology is a useful tool for researchers studying gene expression and cellular functions.

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2 protocols using gata 6

1

Investigating GATA Transcription Factors

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IL-1β was obtained from Alexis Biochemicals (San Diego, CA). JNK-II inhibitor (SP600125, Anthra[1,9-cd]pyrazol-6(2H)-one, 1,9-pyrazoloanthrone), and IKK2-IV (IKK2 inhibitor IV, [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide) were obtained from EMD Chemicals (San Diego, CA) and dissolved in dimethyl sulfoxide (DMSO). Antibodies against GATA-1, GATA-6, GAPDH and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Affinity-purified anti-Rgs4 antibody was kindly provided by Dr. Susanne M. Mumby (University of Texas Southwest Medical Center). Adenovirus (Ad) encoding EGFP, GATA-1 and GATA-6 were obtained from Vector Biolabs (Philadelphia, PA). All the other reagents were from Sigma (St. Louis, MO).
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2

Immunoblot Analysis of Colon Tissue

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Immunoblot analyses in frozen colon tissues were performed using a protocol described previously [19 (link)] using specific primary antibodies (phospo-Pak1, Abcam, Cat# ab40795; Pak1, Abcam, Cat# ab131522; phospo-p38, Santa Cruz Biotechnology, Dallas, TX, Cat# sc-166182; p38, Santa Cruz Biotechnology, Cat# sc-7972; phospo-Gata6, Biorbyt, Cat# orb5304; Gata6, Santa Cruz Biotechnology, Cat# sc-9055; Hif1α: Thermo Fisher Scientific, Waltham, MA, Cat# PA3–16521; cyclin D1, Thermo Fisher Scientific, Cat# RM-2113-S0); β-catenin, Cell Signaling Technologies, Danvers, MA, Cat# 9587s; Nox1, Abcam, Cat# ab55831; Nox3, Abcam, Cat# ab82708; p22phox, Abcam, Cat# ab75941; Noxa1, Santa Cruz Biotechnology, Cat# sc-398873; p47phox, Abcam, Cat# ab176483; β-Tubulin, Santa Cruz Biotechnology, Cat# sc-9104).
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