Specially coated cell culture flasks

Isolated human colon CSCs positive for cancer stem cell markers CD133, CD44, CD34, aldehyde dehydrogenase, telomerase, Sox2, cKit, and Lin28, were obtained from Celprogen Inc. (San Pedro, CA). To maintain the cells in their undifferentiated state, colon CSCs maintenance media and specially coated cell culture flasks obtained from Celprogen were used. Cells were maintained in incubation at 37 °C and 5 % CO₂. Cell cultures at approximately 80 % confluence were used for all in vitro experimental procedures. For all experiments low passage number (less than 10) cells were used (not more than three weeks after resuscitation). The authentication information for the cell line obtained from Celprogen is available under supplemental information.

Human colon cancer stem cells (HCCSCs, positive for cancer stem cell markers CD133, CD44, CD34, aldehyde dehydrogenase, telomerase, Sox2, cKit, and Lin28) were obtained from Celprogen Inc. (San Pedro, CA). To maintain the cells in their undifferentiated state, HCCSCs maintenance media (10% FBS) and specially coated cell culture flasks (Celprogen) were used. Cells were maintained at 37°C and 5% CO₂. Cell cultures at approximately 80% confluence were used for all in vitro experimental procedures.
Human colon cancer cell lines HCT116 p53^+/+ and HCT116 p53^−/− were a gift from Dr. Bert Vogelstein (School of Medicine, Johns Hopkins University, Baltimore, MD). Cells were maintained at 37°C in a humidified atmosphere with 5% CO₂ as described earlier [21 ].

Colon cancer cell lines HCT-116 were a generous gift from Bert Vogelstein (School of Medicine, Johns Hopkins University, Baltimore, MD, USA). Cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂ and grown in McCoy’s F-12 supplemented with 10% FBS, 2.2 g/L sodium bicarbonate, 0.2 g/L bovine serum albumin and 10 mL/L streptomycin-penicillin mix as described earlier [36 (link)].
Colon cancer stem cells (colon CSCs), positive for cancer stem cell markers CD 133, CD 44, and ALDH1b1, were obtained from Celprogen (San Pedro, CA, USA). To maintain the cells in their undifferentiated state, colon CSCs maintenance media and specially coated cell culture flasks obtained from Celprogen were used. Cells were maintained in incubation at 37 °C and 5% CO₂ as described earlier [37 ]. Cell cultures at approximately 80% confluence were used for all in vitro experimental procedures.