To detect SARS-CoV-2 virus particles, either nasopharyngeal swabs (Copan Liquid Amies eSwabs) or pharyngeal lavage specimens were analyzed by RT–PCR. Specimens were subjected to cellular lysis and RNA extraction on a MagNA Pure 24 System (Roche) or QiaSymphony (Qiagen). Real-time RT–PCR was conducted using LightCycler Multiplex RNA Virus Master Mix on a Lightcycler 480 RT system (both Roche) or a ViiA7 system (Applied Biosystems). For SARS-CoV-2 analysis, the Sarbecovirus specific LightMix Modular SARS-CoV (COVID-19) E gene assay was used (TIB Molbiol). EAV control (TIB Molbiol) was used as extraction and internal PCR control. All (RT)-PCR reactions were performed according to manufacturer's protocol.
Eav control
Manufactured by TIB Molbiol
Sourced in Germany
The EAV control is a laboratory equipment designed to monitor and control the level of EAV (Endogenous Viral Elements) in biological samples. It is a compact and reliable device that helps researchers and scientists maintain precise control over the EAV levels during various experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Qiasymphony, by Qiagen (2 mentions)
Magna pure 24 system, by Roche (2 mentions)
Liquid amies eswabs, by Copan (2 mentions)
Lightcycler 480 rt system, by Roche (2 mentions)
Viia 7 system, by Thermo Fisher Scientific (2 mentions)
Lightcycler multiplex rna virus master mix, by Roche (2 mentions)
Biofire filmarray system, by bioMérieux (1 mentions)
Lightmix sarbecov e gene, by TIB Molbiol (1 mentions)
Lightcycler 480, by Roche (1 mentions)
3 protocols using eav control
1
SARS-CoV-2 Detection by RT-PCR
2
SARS-CoV-2 Detection by RT-PCR
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To detect SARS-CoV-2 virus particles, either nasopharyngeal swabs (Copan Liquid Amies eSwabs, Brescia, Italy) or pharyngeal lavage specimens were analyzed by RT-PCR. Specimens were subjected to cellular lysis and RNA extraction on a MagNA Pure 24 System (Roche, Mannheim, Germany) or QiaSymphony (Qiagen, Hilden, Germany). Real-time RT-PCR was conducted using LightCycler Multiplex RNA Virus Master Mix on a Lightcycler 480 RT system (both Roche, Mannheim, Germany) or a ViiA7 system (Applied Biosystems, Foster City, USA).
For SARS-CoV-2 analysis, the Sarbecovirus specific LightMix Modular SARS-CoV (COVID-19) E gene assay was used (TIB Molbiol, Berlin, Germany). EAV control (TIB Molbiol, Berlin, Germany) was used as extraction and internal PCR control. Samples from control patients with acute respiratory disease were additionally analyzed molecularly on the Biofire Filmarray system (bioMérieux, Marcy-l’Étoile, France) with the respiratory panel or the pneumonia plus panel. All (RT)-PCR reactions were performed according to manufacturer’s protocol.
For SARS-CoV-2 analysis, the Sarbecovirus specific LightMix Modular SARS-CoV (COVID-19) E gene assay was used (TIB Molbiol, Berlin, Germany). EAV control (TIB Molbiol, Berlin, Germany) was used as extraction and internal PCR control. Samples from control patients with acute respiratory disease were additionally analyzed molecularly on the Biofire Filmarray system (bioMérieux, Marcy-l’Étoile, France) with the respiratory panel or the pneumonia plus panel. All (RT)-PCR reactions were performed according to manufacturer’s protocol.
3
Quantifying SARS-CoV-2 Viral Load by qPCR
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Cycle threshold values for quantifying viral load in naso/oro-pharyngeal swabs was done by qPCR using LightMix® SarbecoV E-gene(Corman et al., 2020 (link)) plus EAV control (TIB Molbiol, Berlin, Germany) in combination with the N-gene (inhouse primer sets in multiplex PCR) on LightCycler® 480 (Roche Diagnostics).
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