Nickel grid 200 mesh

Manufactured by Electron Microscopy Sciences

Sourced in United States

The Nickel Grid 200 mesh is a type of specimen support grid used in electron microscopy. It is made of nickel and has a mesh size of 200 lines per inch, providing a fine grid pattern for sample mounting. The grid serves as a platform to hold and support thin samples for examination under an electron microscope.

Automatically generated - may contain errors

Lab products found in correlation

Leo 906e, by Zeiss (2 mentions) Em 900, by Zeiss (1 mentions) Uranyl acetate, by Electron Microscopy Sciences (1 mentions)

5 protocols using nickel grid 200 mesh

Transmission Electron Microscopy of uEVs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five μl of each uEV preparation were left to adhere onto formvar carbon coated grids (Nickel Grid 200 mesh; Electron Microscopy Sciences, USA) for five minutes followed by five minutes of negative staining with 2% aqueous uranyl acetate. Excess liquids were blotted. Total grid preparation was performed at room temperature. Images were acquired of air-dried grids on the same day at 80 kV using the Zeiss EM 900 instrument (Zeiss, Germany).
We have submitted all relevant data of our uEV experiments to the EV-TRACK knowledgebase (EV-TRACK ID: EV190007) [38 (link)].

Mussack V., Wittmann G, & Pfaffl M.W. (2019). Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing—Enabling robust and non-invasive biomarker research. Biomolecular Detection and Quantification, 17, 100089.

+ Open protocol

+ Expand

Negative Staining of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated EVs were incubated for 5 min on glow discharged formvar carbon-coated grids (Nickel Grid 200 mesh, Electron Microscopy Sciences, Hatfield, PA, USA). The grids were washed three times with H₂O. Negative staining was performed with 0.5% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA). Excess liquid was removed using filter paper. The grids were air dried for 10 min. Samples were imaged using an LEO 906 EM transmission electron microscope (Zeiss, Oberkochen, Germany), operated at an acceleration voltage of 60 kV.

Borchers P., Winnersbach P., Kraemer S., Beckers C., Buhl E.M., Leonhardt S., Rossaint R., Walter M., Breuer T, & Bleilevens C. (2022). Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit. Journal of Clinical Medicine, 11(12), 3431.

+ Open protocol

+ Expand

Negative Staining of Native EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Native EVs were incubated for 30 min on formvar carbon coated grids (Nickel Grid 200 mesh, Electron Microscopy Sciences, Hatfield, PA, USA) and fixed with 3% glutaraldehyde in H₂O (Sigma-Aldrich, St. Louis, MO, USA). Grids were washed five times for 5 min with H₂O. Negative staining was performed with 4% uranyl acetate in 2% methyl cellulose (ratio of 1:9, v/v) for 10 min in the dark and on ice. Excess liquid was removed using a filter paper. The grids were air dried for 10 min. Samples were imaged using a LEO 906 E transmission electron microscope (Zeiss, Oberkochen, Germany), operated at an acceleration voltage of 60 kV.

Borosch S., Dahmen E., Beckers C., Stoppe C., Buhl E.M., Denecke B., Goetzenich A, & Kraemer S. (2017). Characterization of extracellular vesicles derived from cardiac cells in an in vitro model of preconditioning. Journal of Extracellular Vesicles, 6(1), 1390391.

+ Open protocol

+ Expand

EV Morphology and Purity Evaluation by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

TEM was performed to evaluate EV morphology and size, and to assess the purity of enriched EV fractions. Therefore, five μl of freshly isolated EV preparations were loaded onto formvar carbon-coated grids (Nickel Grid 200 mesh; Electron Microscopy Sciences, USA) and left there to adhere for five minutes prior to five minutes of negative staining with 2% aqueous uranyl acetate at room temperature in the dark. Surplus liquids were removed. Images were acquired of air-dried grids on the same day at 80 kV using the Zeiss EM 900 instrument (Zeiss, Germany) equipped with a wide-angle dual-speed 2KCCD camera.

Li L., Görgens A., Mussack V., Pepeldjiyska E., Hartz A.S., Rank A., Schmohl J., Krämer D., Andaloussi S.E., Pfaffl M.W, & Schmetzer H. (2022). Description and optimization of a multiplex bead-based flow cytometry method (MBFCM) to characterize extracellular vesicles in serum samples from patients with hematological malignancies. Cancer Gene Therapy, 29(11), 1600-1615.

+ Open protocol

+ Expand

Negative Staining of Native EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Native EVs were incubated for 5 min on glow-discharged formvar carbon-coated grids (Nickel Grid 200 mesh, Electron Microscopy Sciences, Hatfield, PA, USA). Grids were washed three times with H₂O. Negative staining was performed with 0.5% uranyl. Excess liquid was removed using filter paper. The grids were air dried for 10 min. Samples were imaged using a LEO 906 E transmission electron microscope (Zeiss, Oberkochen, Germany), operated at an acceleration voltage of 60 kV.

Bleilevens C., Beckers C., Theissen A., Fechter T., Buhl E.M., Greven J., Kraemer S, & Wendt S. (2021). Repetitive Treatment with Volatile Anesthetics Does Not Affect the In Vivo Plasma Concentration and Composition of Extracellular Vesicles in Rats. Current Issues in Molecular Biology, 43(3), 1997-2010.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!