Norgen rna extraction kit

Manufactured by Norgen Biotek

Sourced in Canada

Norgen's RNA Extraction Kit is a nucleic acid purification system designed for the rapid and efficient isolation of high-quality RNA from a variety of sample types. The kit utilizes a silica-based membrane technology to selectively bind RNA, while effectively removing contaminants and inhibitors.

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Lab products found in correlation

Qiaamp blood 96 kit, by Qiagen (1 mentions) Fragment analyzer system, by Agilent Technologies (1 mentions) Rnase free dnase 1 kit, by Norgen Biotek (1 mentions) Model 6875, by SPEX (1 mentions) Ab21990, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions)

Close spelling variants of this product

RNA extraction kit (13 citations) Norgen® Extraction kit (2 citations) Norgen total RNA-extraction kit (2 citations)

4 protocols using norgen rna extraction kit

DNA and RNA Extraction from DBS and Whole Blood

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Total DNA was extracted from three 0.32 cm diameter circles punched from each DBS using the QIAamp 96 Blood Kit (Qiagen, Valencia, CA) per the manufacturer’s instructions and eluted in 50 µl EDTA buffer. RNA was extracted from whole-blood RNA in Tempus using Norgen RNA extraction kit (Norgen Biotek, Thorold, Ontario) and treated with RNase-Free DNase I Kit (Norgen Biotek) to a final elution volume of 50 µl, per manufacturer’s instructions. Extracted RNA samples were assessed for quality and quantity using automated parallel capillary electrophoresis (Fragment Analyzer System, Agilent).

Salgado C., Ayodo G., Macklin M.D., Gould M.P., Nallandhighal S., Odhiambo E.O., Obala A., O’Meara W.P., John C.C, & Tran T.M. (2021). The prevalence and density of asymptomatic Plasmodium falciparum infections among children and adults in three communities of western Kenya. Malaria Journal, 20, 371.

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RNA Extraction from Cell Lysate

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Immediately after loading, cells were lysed and total RNA was extracted and column purified according to the Norgen RNA extraction kit protocol (Catalog # 48300; Norgen Biotek Corp., Thorold, Canada).

Andress B.D., Irwin R.M., Puranam I., Hoffman B.D, & McNulty A.L. (2022). A Tale of Two Loads: Modulation of IL-1 Induced Inflammatory Responses of Meniscal Cells in Two Models of Dynamic Physiologic Loading. Frontiers in Bioengineering and Biotechnology, 10, 837619.

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Efficient RNA Extraction from Plant Tissue

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Explants were pulverized in TRIzol (Catalog # 15596026; Life Technologies, Carlsbad, CA) under liquid nitrogen using a freezer mill (Model 6875; SPEX SamplePrep, Metuchen, NJ) for three cycles of 2 min each at maximum frequency with 2 min precooling between cycles. RNA was extracted by TRIzol/chloroform separation, according to the manufacturer’s protocol. RNA was then resuspended in lysis buffer and column purified, according to the Norgen RNA extraction kit protocol (Catalog # 48300; Norgen Biotek Corp).

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Knockdown of OTX2 in Medulloblastoma Cell Lines

Cited 2 times

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OTX2 knock down in HDMB03 and MB3W1 G3 MB cells (2×10⁵ cells/well) was performed as previously described^{33 (link),34 (link)}. Briefly, OTX2 was silenced using 30 nM Silencer Select siRNAs 9931 or 9932 (Life Technologies). A non-silencing (scramble) served as the negative control. For bulk RNA sequencing, OTX2 was knocked down in three independent biological replicates for each cell line and silencing was confirmed by western blot (OTX2, Abcam, ab21990, rabbit, at 1:500, and β-actin, Sigma-Aldrich, A2228, mouse, at 1/1000 was used as a loading control) 72 hours following transfection. Total RNA was extracted from all samples using the Norgen RNA extraction kit (Norgen Biotek), and bulk RNA sequencing (RNAseq) was performed by StemCore laboratories at the Ottawa Hospital Research Institute (Ottawa, ON, Canada). For single-nucleus RNA sequencing (snRNAseq), the above was repeated but using 4.5X10⁵cells/well for MB3W1 and only one replicate was performed. Granule neuron differentiation was validated by western blot for RBFOX3 (NeuN) (Cell Signaling Technology, D4G4O, at 1:1000).

Johjima T., Itoh N., Kabuto M., Tokimura F., Nakagawa T., Wariishi H, & Tanaka H. (1999). Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. Proceedings of the National Academy of Sciences of the United States of America, 96(5), 1989-1994.

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