Mgcl2 6h2o

Manufactured by Nacalai Tesque

Sourced in Japan

MgCl2·6H2O is a chemical compound that consists of magnesium, chlorine, and water molecules. It is a crystalline solid that is commonly used as a laboratory reagent.

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Lab products found in correlation

7 protocols using mgcl2 6h2o

Surface Modification and Characterization of Titanium Alloys

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A commercially pure, medical-grade Ti plate (ISO5832-2) (Ti > 99.5%) was provided by Nilaco Co., Tokyo, Japan. Ti-6Al-4V alloy plate (Ti = balance, Al = 6.18, V = 4.27 mass%) and Ti-15Zr-4Nb-4Ta alloy plate (Ti = balance, Zr = 14.51, Nb = 3.83, Ta = 3.94, Pd = 0.16, and O = 0.25 mass%) were supplied by Kobelco Research Institute, Inc., Hyogo, Japan.
The chemical reagents (NaOH, CaCl₂, ICl₃, ICl, NaI and povidone iodine (PVP-I)) used for surface treatment were reagent-grade and purchased from Kanto Chemical Co., Inc., Tokyo, Japan. Reagent-grade NaCl, NaHCO₃, KCl, K₂HPO₄·3H₂O, MgCl₂·6H₂O, CaCl₂, Na₂SO₄, tris-hydroxymethylaminomethane (CH₂OH)₃CNH₂, and 1 M HCl were purchased from Nacalai Tesque, Inc., Kyoto, Japan, and used for the preparation of simulated body fluid (SBF). Minimum essential medium (MEM) was obtained from Gibco, Thermo Fisher Scientific, Waltham, MA, USA, and used for the cell culture test. Nutrient broth (Eiken Chemical Co., Ltd. Tochigi, Japan) and RPMI 1640 (Fujifilm Wako Pure Chemical Corp., Osaka, Japan) were used for the antibacterial activity test.

Yamaguchi S., Le P.T., Shintani S.A., Takadama H., Ito M., Ferraris S, & Spriano S. (2021). Iodine-Loaded Calcium Titanate for Bone Repair with Sustainable Antibacterial Activity Prepared by Solution and Heat Treatment. Nanomaterials, 11(9), 2199.

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Porcine Oocyte Maturation Protocol

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NaCl, KCl, KH₂PO₄, MgCl₂·6H₂O, CaCl₂·2H₂O, and gentamicin sulfate were purchased from Nacalai
Tesque (Kyoto, Japan). MgSO₄·7H₂O was purchased from Ishizu Pharmaceutical (Osaka, Japan). Furthermore, eCG (the trade name; Serotropin)
and hCG (the trade name; Gonatropin) were purchased from ASKA Pharmaceutical (Tokyo, Japan). Unless otherwise specified, other chemicals were purchased from
Sigma Aldrich (St. Louis, MO, USA).
Modified TL-HEPES-PVA medium composed of 114 mM NaCl, 3.2 mM KCl, 2 mM NaHCO₃, 0.34 mM NaH₂PO₄, 10 mM Na-lactate, 0.5 mM
MgCl₂·6H₂O, 2 mM CaCl₂·2H₂O, 12 mM sorbitol, 10 mM HEPES, 0.2 mM Na-pyruvate, 0.1% (w/v) polyvinyl alcohol (PVA),
25 µg/ml gentamicin sulfate, and 65 µg/ml potassium penicillin G was used for collecting and washing COCs. The basic IVM medium was a BSA-free,
chemically-defined, porcine oocyte medium (POM, Research Institute for the Functional Peptides, Yamagata, Japan) supplemented with 50 µM beta-mercaptoethanol
(mPOM) [20 (link)]. This IVM medium was equilibrated at 39°C in an atmosphere of 5% CO₂ overnight prior to use.

OKUDAIRA Y., WAKAI T, & FUNAHASHI H. (2017). Levels of cyclic-AMP and cyclic-GMP in porcine oocyte-cumulus complexes and cumulus-free oocytes derived from small and middle follicles during the first 24-hour period of in vitro maturation. The Journal of Reproduction and Development, 63(2), 191-197.

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Apatite Formation on Porous Titanium

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Cylindrical specimens of the porous Ti metals subjected to the various treatments given in Table 1 were broken and soaked in 30 mL of a simulated body fluid (SBF) having ion concentrations close to those of human blood plasma (Na⁺ = 142.0, K⁺ = 5.0, Mg²⁺ = 1.5, Ca²⁺ = 2.5, Cl^– = 147.8, HCO₃^– = 4.2, HPO₄^2– = 1.0, and SO₄^2– = 0.5 mM) [19] (link), [20] (link) at 36.5°C. The SBF was prepared by dissolving reagent-grade NaCl, NaHCO₃, KCl, K₂HPO₄·3 H₂O, MgCl₂.6 H₂O, CaCl₂, and Na₂SO₄ (Nacalai Tesque Inc., Japan). After 1 day, the specimens were removed from the SBF solution, gently washed with ultrapure water and dried in an oven at 40°C. Formation of apatite on their surfaces was examined by SEM and TF-XRD analysis.

Kawai T., Takemoto M., Fujibayashi S., Akiyama H., Tanaka M., Yamaguchi S., Pattanayak D.K., Doi K., Matsushita T., Nakamura T., Kokubo T, & Matsuda S. (2014). Osteoinduction on Acid and Heat Treated Porous Ti Metal Samples in Canine Muscle. PLoS ONE, 9(2), e88366.

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Simulated Body Fluid Soaking Protocol

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The samples subjected to the MA-heat or NaOH-HCl-heat treatments were soaked in 24 mL of a simulated body fluid (SBF) with various ion concentrations (Na⁺ 142.0, K⁺ 5.0, Ca²⁺ 2.5, Mg²⁺ 1.5, Cl⁻ 147.8, HCO₃⁻ 4.2, HPO₄²⁻ 1.0, and SO₄²⁻ 0.5 mM) closed to human blood plasma at 36.5 °C. The SBF was prepared by dissolving reagent grade NaCl, NaHCO₃, KCl, K₂HPO₄•3H₂O, MgCl₂•6H₂O, CaCl₂, and Na₂SO₄ (Nacalai Tesque Inc., Kyoto, Japan) in ultrapure water, and buffered at pH = 7.4 with tris(hydroxymethyl)aminomethane (CH₂OH)₃CNH₂ and 1 M HCl (Nacalai Tesque Inc., Kyoto, Japan) at 36.5 °C [8 (link)]. After soaking in the SBF for 3 and 7 days, the samples were gently rinsed with ultrapure water, and dried.

Yamaguchi S., Hashimoto H., Nakai R, & Takadama H. (2017). Impact of Surface Potential on Apatite Formation in Ti Alloys Subjected to Acid and Heat Treatments. Materials, 10(10), 1127.

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Bovine Oocyte In Vitro Fertilization

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After in vitro maturation, the COCs were transferred into a 15 ml tube containing 2 ml of HEPES-TLP-PVA supplemented with 0.1% (w/v) hyaluronidase (Sigma) and vortexed for
90 sec to remove cumulus cells from oocytes. Denuded oocytes were transferred to droplets (80 µl) of fertilization medium in groups of 15 to 20 under paraffin oil in a 35 mm polystyrene
dish. The fertilization medium was composed of 114.0 mM NaCl (Nacalai), 3.2 mM KCl (Nacalai), 6.76 mM CaCl₂·2H₂O (Nacalai), 0.5 mM MgCl₂·6H₂O
(Nacalai), 0.1 mM sodium pyruvate, 10.0 mM sodium lactate (Sigma), 0.35 mM NaH₂PO₄·2H₂O (Nacalai), 5.0 mM D-glucose, 25.0 mM NaHCO₃ (Nacalai),
0.3% (w/v) bovine serum albumin (Fraction V; Sigma), 100 µg/ml amikacin sulfate, and 2.0 mM caffeine (Sigma). The frozen spermatozoa were thawed immediately before insemination, as described
above. HEPES-TLP-PVA containing frozen-thawed spermatozoa was centrifuged at 760 ×g for 10 min at 38°C, and the supernatant was removed. The precipitated spermatozoa were
gently suspended in the fertilization medium at a concentration of 3.5 × 10⁷ spermatozoa/ml, and 20 µl of this sperm suspension was introduced into the 80 µl droplet that
contained denuded oocytes at a final concentration of 7.0 × 10⁶ spermatozoa/ml. The oocytes and spermatozoa were then cultured for 12 h at 38.5°C in an atmosphere with 5%
CO₂ in air.

JIN H., CHOI W., MATSUMURA K., HYON S.H., GEN Y., HAYASHI M., KAWABATA T., IJIRI M, & MIYOSHI K. (2022). Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant. The Journal of Reproduction and Development, 68(5), 312-317.

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In-vitro Biomineralization of VBSO2 and VBSO3

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The cylindrical VBSO₂ 2.5Ca
and VBSO₃ 2.5Ca specimens were soaked in 30 cm³ of SBF (Na⁺ 142.0, K⁺ 5.0, Mg²⁺ 1.5, Ca²⁺ 2.5, Cl^– 147.8, HCO₃^– 4.2, HPO₄^2– 1.0,
SO₄^2– 0.5 mol·m^–3, pH 7.40) for various time intervals. In addition, 9 mg of powdered
VBSO₂ 0Ca and VBSO₃ 0Ca specimens was soaked
in SBF1.5Ca, which has Ca²⁺ concentration 1.5 times that
of the normal SBF^{36 (link)} with a pH 7.25 at 36.5
°C for various time intervals. The powder was prepared by cooling
the bulk specimen using liquid N₂ and pulverizing it with
a porcelain mill and agate balls.
SBF and SBF1.5Ca were prepared
by dissolution of NaCl, NaHCO₃, KCl, K₂HPO₄·3H₂O, MgCl₂·6H₂O, CaCl₂, and Na₂SO₄ (Nacalai Tesque,
Inc., Kyoto, Japan) in ultrapure water in that order. The pH for each
resulting solution was adjusted by the addition of 1 kmol·m^–3 HCl solution and Tris (Nacalai Tesque, Inc.).

Hamai R., Shirosaki Y, & Miyazaki T. (2018). Structural Effects of Sulfur-Containing Functional Groups on Apatite Formation on Ca2+-Modified Copolymers in a Simulated Body Environment. ACS Omega, 3(5), 5627-5633.

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Evaluating Apatite Formation in Simulated Body Fluid

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Apatite formation of the samples after the chemical and heat treatments were examined by soaking in 24 mL of SBF that has ion concentrations (Na⁺ 142.0, K⁺ 5.0, Ca²⁺ 2.5, Mg²⁺ 1.5, Cl⁻ 147.8, HCO₃⁻ 4.2, HPO₄²⁻ 1.0, and SO₄²⁻ 0.5 mM) nearly equal to those of human blood plasma at 36.5 °C for various periods of up to 7 days. The SBF was prepared by dissolving reagent grade NaCl, NaHCO₃, KCl, K₂HPO₄·3H₂O, MgCl₂·6H₂O, CaCl₂, and Na₂SO₄ (Nacalai Tesque Inc., Kyoto, Japan) in ultrapure water, and then buffered at pH = 7.4 with tris(hydroxymethyl)aminomethane (CH₂OH)₃CNH₂ and 1 M HCl (Nacalai Tesque Inc., Kyoto, Japan) at 36.5 °C [50 (link)]. After being removed from the SBF, the samples were gently rinsed with ultrapure water and dried, then their surface apatite formation was analysed using TF-XRD, FE-SEM and EDX.

Yamaguchi S., Nath S., Sugawara Y., Divakarla K., Das T., Manos J., Chrzanowski W., Matsushita T, & Kokubo T. (2017). Two-in-One Biointerfaces—Antimicrobial and Bioactive Nanoporous Gallium Titanate Layers for Titanium Implants. Nanomaterials, 7(8), 229.

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