The resistant strain used in this study was E. coli DH5α transformed with the pUA66-GFP plasmid, which harbors a gene encoding extended-spectrum β-lactamase (CTX-M) and a gene encoding GFP. The nutritional strain was E. coli MG1655 transformed with a plasmid harboring a gene encoding RFP. More Information regarding the genotypes, phenotypes, and sources of the bacterial strains and plasmids used in this study are listed in Supplementary Table 1 . Tryptone and yeast extract were purchased from Oxoid, Ltd. Agar was purchased from Sigma, and NaCl and lactose were obtained from Sinopharm Chemical Reagent Co., Ltd. PBS was purchased from Shanghai Double-Helix Biotech Co., Ltd., and M9 was obtained from Leagene. CRO and kanamycin were purchased from Rocephin, and ampicillin sodium salt was purchased from Amresco.
Lactose
Manufactured by Sinopharm
Sourced in China
Lactose is a primary carbohydrate found in milk and dairy products. It serves as a source of energy and plays a vital role in various biochemical processes. This lab equipment product provides a reliable and consistent supply of lactose for use in research, analysis, and various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sucrose, by Sinopharm (8 mentions)
Glucose, by Sinopharm (8 mentions)
Maltose, by Sinopharm (6 mentions)
Fructose, by Sinopharm (6 mentions)
Sodium chloride (nacl), by Sinopharm (5 mentions)
Soluble starch, by Sinopharm (3 mentions)
Cacl2, by Sinopharm (3 mentions)
Citric acid, by Sinopharm (3 mentions)
Sodium hydroxide (naoh), by Sinopharm (3 mentions)
N hexane, by Sinopharm (2 mentions)
Ethanol, by Sinopharm (2 mentions)
Galactose, by Sinopharm (2 mentions)
Boric acid, by Sinopharm (2 mentions)
L cysteine, by Sinopharm (2 mentions)
Imidazole, by Sinopharm (2 mentions)
Glycerol, by Sinopharm (2 mentions)
Ampicillin, by Solarbio (2 mentions)
Na2co3, by Sinopharm (2 mentions)
Xylose, by Sinopharm (2 mentions)
Sodium acetate, by Sinopharm (2 mentions)
17 protocols using lactose
1
Antibiotic-Resistant E. coli Transformation
2
Enzymatic Synthesis of Fatty Acid Esters
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Lipozyme TLIM was purchased from Novo Nordisk (Denmark). Molecular sieves (4 Å), 2-methylbutanol, dimethylsulfoxide (DMSO), sucrose, maltose, lactose and n-hexane were from Sinopharm (China). Raffinose pentahydrate was from Alfa Aesar (France). Vinyl laurate and 6-O-monolaurate were from Sigma (Denmark). Vinyl octanoate and vinyl decanoate were purchased from TCI (Japan). Ryoto sucrose ester (L1695) was supplied by Mitsubishi-Kasei Food Corporation (Japan). All the reagents and solvents were of analytical grade. Vinyl fatty acid esters, 2-methyl-2-butanol and DMSO were stored over molecular sieves (4 Å), at least 24 h prior to use. Double-distilled water with a surface tension equal to 69.3 mN/m at 25°C was used in all experiments.
3
Recombinant γ-CGTase Production in E. coli
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The E. coli BL21(DE3) expression strain used in this study harbored the recombinant plasmid cgt/pET24a (+), in which a sequence encoding the OmpA signal peptide is fused to the cgt gene encoding γ-CGTase from Bacillus clarkii 7364 (Accession No.: BAH14968.1). Yeast extract and tryptone were purchased from Oxoid Co., Ltd., while α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, soluble starch, bromocresol green, lactose, isopropyl β-D-1-thiogalactopyranoside (IPTG) and all other chemicals and reagents were purchased from Sinopharm Chemical Reagent (Shanghai, China) Co., Ltd.
4
Oligonucleotides Synthesis and Purification
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The synthetic oligonucleotides DNA purified by PAGE was obtained from Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China). SYBR Green I was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Melamine, lactose, glucose, sucrose, CaCl2, CuCl2, FeCl3, KCl, MgCl2, MnCl2, vitamin B2 and vitamin C were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Exonuclease I was purchased from Takara Biotechnology Co. Ltd. (Dalian, China). All chemicals were analytical reagent. The water used was purified by Millipore (Burlington, MA, USA) Milli-Q (18 MΩ/cm). Stock solution of oligonucleotides (100 μM) was prepared by deionized water. Before used, the oligonucleotides solution was diluted to the required concentration with deionized water.
5
Comprehensive Analytical Reagents Protocol
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Tetrabutyl orthotitanate (TBOT), boric acid, ammonium chloride, disodium phosphate, sodium dihydrogen phosphate, methanol, ethanol, acetonitrile, 3,3′,5,5′-tetramethyl benzidine, glucose, fructose, maltose, galactose, lactose, citric acid were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Uric acid, lauric acid, ascorbic acid, L-aspartic acid were purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA), Human serum albumin (HSA), horse radish peroxidase, glucose oxidase, L-cysteine, glutathione, L-phenylalanine, glycine, L-tryptophan, L-methionine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human serum was purchased from Solarbio (Beijing, China).
6
Isolation and Characterization of E. coli O157:H7
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The materials including sucrose, sulfuric acid (H2SO4), sodium hydroxide (NaOH), lactose, bovine bile salt, sodium carboxymethyl cellulose, sodium chloride, phosphoric acid, acetic acid, boric acid, and dipotassium phosphate were purchased from Sinopharm Chemical Reagent Co. Ltd., China (https://www.sinoreagent.com ). Peptone was supplied by Shanghai Shengsi Biochemical Technology Co. Ltd., China (http://ssincere.com ). Yeast extract was purchased from the Thermo Fisher Oxoid Co. Ltd., England (https://www.thermofisher.com ). Agar was provided by the Biosharp Life Sciences Co. Ltd., China (http://www.biosharp.cn ). The E. coli O157:H7 (CCTCC AB 200051) was obtained from the Center for Type Culture Collection, China (http://www.cctcc.org ). All reagents used were of analytical grade and used without further processing.
7
Electrochemical Sensing of Bioanalytes
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A Cu electrode, Ag/AgCl electrode, and Pt electrode were purchased from Aidahengsheng, Tianjin, China. Ascorbic acid (99%, analytical pure) was purchased from Solarbio, Beijing, China. Lactose (99%, analytical pure) was purchased from Sinopharm Chemical Reagent, Beijing, China. Dopamine (99%, analytical pure) and uric acid (99%, analytical pure) were purchased from Aladdin, Shanghai, China. All agents were used without further purification.
8
Electrochemical Characterization of Carbohydrates
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Nickel disk electrode (diameter of 2 mm and length of 20 mm) was acquired from Tianjin Aida Hengsheng Technology Co., Ltd. (Tianjin, China). Potassium ferricyanide, potassium chloride, sodium hydroxide, glucose, sucrose, fructose, lactose, maltose, xylitol, sorbitol, and erythritol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the chemical reagents were used as received. All solutions were prepared using Milli-Q water, with resistivity of 18.2 MΩ·cm using a Millipore system.
9
Synthesis and Characterization of Metallic Nanoparticles
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Hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O), NaBH4 and TMB were purchased from Sigma-Aldrich (Shanghai, China). Fetal bovine serum, glucose oxidase (GOX), H2O2, sodium citrate, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), CuSO4·5H2O, l -ascorbic acid, glucose, lactose, maltose, sucrose, d -mannitol, l -cysteine and other kinds of amino acids were purchased from Sinopharm Chemical Reagent Co.,Ltd (Shanghai, China). K2PtCl6 was purchased from Aladdin (Shanghai, China). AgNO3 was purchased from Energy Chemical (Shanghai, China). All the solutions were prepared with distilled water.
10
Heterologous Expression of β-Glucosidase
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E. coli BL21(DE3) and plasmid pET-15b (Novagen, Madison, WI, USA) were used in this study. Ampicillin was purchased from Solarbio Co. Ltd. (Beijing, China). Tris, glycerol, imidazole, citric acid, NaCl, Na2CO3, CaCl2, glucose, sucrose, lactose, and soluble starch were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). The yeast extract and tryptone used in this study were produced by Oxoid (Basingstoke, Great Britain). Geniposide and p-nitrophenyl-β-d -glucopyranoside (pNPG) were purchased from Yuanye Co. Ltd. (Shanghai, China).
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