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Mouse erythropoietin elisa kit

Manufactured by Abcam
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The Mouse Erythropoietin ELISA Kit is a quantitative test used to measure the concentration of erythropoietin (EPO) in mouse samples. It employs the quantitative sandwich enzyme immunoassay technique.

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Mouse ELISA kit (28 citations) ELISAs (10 citations) Erythropoietin (2 citations)

3 protocols using mouse erythropoietin elisa kit

1

Serum EPO Levels in Genetically Altered Mice

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The serum was collected from mice of different genotypes at 2 weeks after gene deletion and Mouse Erythropoietin ELISA Kit (Abcam, ab270893) was used for EPO level detection. Each sample were assayed with two replicates and the OD was recorded at 450nm as endpoint reading.
Tu Z., Fan C., Davis A.K., Hu M., Wang C., Dandamudi A., Seu K.G., Kalfa T.A., Lu Q.R, & Zheng Y. (2022). Autism-associated chromatin remodeler CHD8 regulates erythroblast cytokinesis and fine-tunes the balance of Rho GTPase signaling. Cell reports, 40(2), 111072.
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2

Serum EPO Levels in Genetically Altered Mice

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The serum was collected from mice of different genotypes at 2 weeks after gene deletion and Mouse Erythropoietin ELISA Kit (Abcam, ab270893) was used for EPO level detection. Each sample were assayed with two replicates and the OD was recorded at 450nm as endpoint reading.
Tu Z., Fan C., Davis A.K., Hu M., Wang C., Dandamudi A., Seu K.G., Kalfa T.A., Lu Q.R, & Zheng Y. (2022). Autism-associated chromatin remodeler CHD8 regulates erythroblast cytokinesis and fine-tunes the balance of Rho GTPase signaling. Cell reports, 40(2), 111072.
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3

Mouse Erythropoietin Measurement Protocol

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Blood samples were collected by puncture of the submandibular vein.
Serum EPO concentrations were determined using Mouse Erythropoietin ELISA Kit (#ab270893, Abcam, Waltham, MA) following the manufacturer’s instructions.
To measure hematocrit levels, samples were spun down for 60 s in conventional hematocrit capillary tubes using HemataStat II™ Hematocrit Centrifuge (EKF Diagnostics Inc., San Antonio, TX).
Bin S., Cantarelli C., Horwitz J.K., Gentile M., Podestà M.A., La Manna G., Heeger P.S, & Cravedi P. (2023). Endogenous erythropoietin has immunoregulatory functions that limit the expression of autoimmune kidney disease in mice. Frontiers in Immunology, 14, 1195662.
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