Log In Sign up
The largest database of trusted experimental protocols

Mibolerone

Manufactured by Merck Group
Visit Supplier

Mibolerone is a synthetic anabolic-androgenic steroid. It is used in laboratory research and development settings. The core function of Mibolerone is to serve as a research tool for studying the effects of anabolic-androgenic steroids.

Automatically generated - may contain errors

Lab products found in correlation

Anti ar n 20, by Santa Cruz Biotechnology (2 mentions) Anti myc tag, by Santa Cruz Biotechnology (2 mentions) Anti spop, by Abcam (2 mentions) Anti erk2, by Santa Cruz Biotechnology (2 mentions) Mg132, by Merck Group (2 mentions) Anti ha, by Fortrea (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Kod plus mutagenesis kit, by Toyobo (2 mentions) Bicalutamide, by Merck Group (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Lncap, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Troglitazone, by Cayman Chemical (1 mentions) Dihydrotestosterone, by Merck Group (1 mentions) Enzalutamide, by Selleck Chemicals (1 mentions) Medroxyprogesterone acetate, by Merck Group (1 mentions) Progesterone, by Merck Group (1 mentions) Adione, by Merck Group (1 mentions) R1881, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions)

5 protocols using mibolerone

1

Mammalian Expression Vectors for AR and its Variants

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The mammalian expression vectors for HA-tagged ubiquitin (HA-Ub), wild-type AR (AR-WT), and AR variants V2, V4, V5, and V7 were described previously (Bohrer et al., 2013 (link); Huang et al., 2005 (link); Liu et al., 2008 (link)). The AR variant v567es was kindly provided by Dr. Donald Tindall at Mayo Clinic. HA-SPOP and Myc-SPOP were cloned into pCMV vector. AR mutants S646A, S647A, T648A, and T649A and prostate-cancer-associated mutants of SPOP were generated by site-specific mutagenesis (Stratagene). AR deletion mutants ΔEGSSS, ΔASSTT, 2Δ, and Myc-SPOP-ΔBTB were generated by KOD-Plus Mutagenesis Kit (Toyobo). Antibodies used were anti-AR (N20), anti-ERK2, anti-Myc tag (Santa Cruz Biotechnology), anti-SPOP (Abcam), and anti-HA (Covance). MG132, cycloheximide, and mibolerone were purchased from Sigma-Aldrich. The antiandrogen enzalutamide was kindly provided by Medivation.
An J., Wang C., Deng Y., Yu L, & Huang H. (2014). Destruction of Full-Length Androgen Receptor by Wild-Type SPOP, but Not Prostate-Cancer-Associated Mutants. Cell reports, 6(4), 657-669.
+ Open protocol
+ Expand
2

Cell Line Acquisition and Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
LNCaP, MCF-7 and HepG2 cell lines were purchased from ATCC. C4-2 cell line was purchased from UroCorporation. Mibolerone and bicalutamide were purchased from Sigma-Aldrich. Enzalutamide was kindly provided by Medivation.
Zhao Y., Wang L., Ren S., Wang L., Blackburn P.R., McNulty M.S., Gao X., Qiao M., Vessella R.L., Kohli M., Zhang J., Karnes R.J., Tindall D.J., Kim Y., MacLeod R., Ekker S.C., Kang T., Sun Y, & Huang H. (2016). Activation of P-TEFb by Androgen Receptor-Regulated Enhancer RNAs in Castration-Resistant Prostate Cancer. Cell reports, 15(3), 599-610.
+ Open protocol
+ Expand
3

Mammalian Expression Vectors for AR and its Variants

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The mammalian expression vectors for HA-tagged ubiquitin (HA-Ub), wild-type AR (AR-WT), and AR variants V2, V4, V5, and V7 were described previously (Bohrer et al., 2013 (link); Huang et al., 2005 (link); Liu et al., 2008 (link)). The AR variant v567es was kindly provided by Dr. Donald Tindall at Mayo Clinic. HA-SPOP and Myc-SPOP were cloned into pCMV vector. AR mutants S646A, S647A, T648A, and T649A and prostate-cancer-associated mutants of SPOP were generated by site-specific mutagenesis (Stratagene). AR deletion mutants ΔEGSSS, ΔASSTT, 2Δ, and Myc-SPOP-ΔBTB were generated by KOD-Plus Mutagenesis Kit (Toyobo). Antibodies used were anti-AR (N20), anti-ERK2, anti-Myc tag (Santa Cruz Biotechnology), anti-SPOP (Abcam), and anti-HA (Covance). MG132, cycloheximide, and mibolerone were purchased from Sigma-Aldrich. The antiandrogen enzalutamide was kindly provided by Medivation.
An J., Wang C., Deng Y., Yu L, & Huang H. (2014). Destruction of Full-Length Androgen Receptor by Wild-Type SPOP, but Not Prostate-Cancer-Associated Mutants. Cell reports, 6(4), 657-669.
+ Open protocol
+ Expand
4

Characterization of Bisphenol A Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dihydrotestosterone (DHT), mibolerone (MIB), and BABDHE (Bisphenol A bis [2,3-dihydroxypropyl] ether) were purchased from Sigma. Enzalutamide (ENZ) was purchased from Selleck Chemicals. Troglitazone was purchased from Cayman Chemical. EPI-001 (Bisphenol A [3-chloro-2-hydroxypropyl] [2,3-dihydroxypropyl] ether) was synthesized (See Supplementary Methods) or purchased from commercial sources (Santa Cruz Biotechnology or Sigma-Aldrich). EPI-001 was analyzed for purity via HPLC and NMR (Supplementary Methods, Supplementary Figures S1 and S2). EPI-001 dissolved in DMSO was used for all experiments (Supplementary Methods, Supplementary Figure S3). All other drugs were suspended in DMSO with the exception of DHT, MIB, and ENZ, which were prepared in absolute ethanol. Final DMSO or ethanol concentrations did not exceed 0.1% (v/v) in culture medium.
Brand L.J., Olson M.E., Ravindranathan P., Guo H., Kempema A.M., Andrews T.E., Chen X., Raj G.V., Harki D.A, & Dehm S.M. (2015). EPI-001 is a selective peroxisome proliferator-activated receptor-gamma modulator with inhibitory effects on androgen receptor expression and activity in prostate cancer. Oncotarget, 6(6), 3811-3824.
+ Open protocol
+ Expand
5

Hormone and Compound Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
T, DHT, Adiol, Adione, R1881 (methyltrienolone), mibolerone, medroxyprogesterone acetate, E2, progesterone, dexamethasone, hydrocortisone, DHEA and DHEA-sulphate, ethanol, dimethylsulfoxide (DMSO) and ionomycin were purchased from Sigma-Aldrich. was a kind gift from dr. N. Kumar of the Population Council (New York, NY, U.S.A.). Enzalutamide was purchased from Sequoia Research Products (Pangbourne, U.K.).
Laurent M.R., Helsen C., Antonio L., Schollaert D., Joniau S., Vos M.J., Decallonne B., Hammond G.L., Vanderschueren D, & Claessens F. (2016). Effects of sex hormone-binding globulin (SHBG) on androgen bioactivity in vitro. Molecular and cellular endocrinology, 437.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!