Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100× 1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with a MLC400B laser launch (Agilent), with 405 nm, 488 nm, 561 nm and 640 nm laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition is controlled by NIS Elements Advanced Research software (Nikon).
Plano apo
Manufactured by Nikon
The Plano Apo is a high-quality optical lens designed for laboratory and scientific applications. It features a planar design and apochromatic (Apo) correction to provide precise, distortion-free images with excellent color accuracy. The Plano Apo lens is suitable for a variety of microscopy and imaging tasks, but its specific intended use is not provided in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Eclipse ti e, by Nikon (5 mentions)
Ixon ultra 888, by Oxford Instruments (5 mentions)
Mlc400b laser launch, by Agilent Technologies (5 mentions)
Nis elements advanced research software, by Nikon (2 mentions)
Ti2 microscope, by Nikon (1 mentions)
W1 confocal scanhead, by Nikon (1 mentions)
Apo tirf 100 1.49 na objective, by Nikon (1 mentions)
6 protocols using plano apo
1
Multicolor Fluorescence Microscopy Imaging
2
High-resolution Fluorescence Microscopy Setup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imaging was performed with an inverted microscope (Ti-E Eclipse; Nikon) equipped with a 100× 1.49 NA oil immersion objective (Plano Apo; Nikon). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with an MLC400B laser launch (Agilent) equipped with 405-nm (30 mW), 488-nm (90 mW), 561-nm (90 mW), and 640-nm (170 mW) laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying charge coupled device camera (iXon Ultra 888; Andor Technology). Illumination and image acquisition were controlled by NIS Elements Advanced Research software (Nikon).
3
Inverted Microscope Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100×1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with an MLC400B laser launch (Agilent) equipped with 405 nm (30 mW), 488 nm (90 mW), 561 nm (90 mW), and 640 nm (170 mW) laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition was controlled by NIS Elements Advanced Research software (Nikon).
4
Visualizing Yeast Spindle Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Single colonies were picked and grown at 30 °C. Log-phase live S. cerevisiae cells were mounted on a thin agarose pad made from SC medium pressed between two glass slides. Live cells genetically modified to express fluorescently labeled DYN1-3XGFP, CFP-TUB1, and SPC110-tdTomato were imaged using a Yokogawa W1 confocal scanhead mounted to a Nikon Ti2 microscope with an Apo TIRF 100 ×1.49 NA objective (Nikon, Plano Apo). The microscope was run with NIS Elements using the 488 nm 515 nm and 561 nm lines of a six-line (405 nm, 445 nm, 488 nm, 515 nm, 561 nm, and 640 nm) LUN-F-XL laser engine and Prime95B cameras (Photometrics). The DYN1-3×GFP foci localizing to the spindle pole body (SPB), microtubule plus end, and cell cortex were outlined as regions of interest in Fiji66 (link), recorded, and analyzed for three replicates of at least 120 cells for each sample.
5
Inverted Fluorescence Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100 × 1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with an MLC400B laser launch (Agilent) equipped with 405 nm (30 mW), 488 nm (90 mW), 561 nm (90 mW), and 640 nm (170 mW) laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition were controlled by NIS Elements Advanced Research software (Nikon).
6
Temperature-controlled Microscopy Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100 3 1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by a Pro-Scan linear motor stage controller (Prior). The microscope was equipped with a MLC400B laser launch (Agilent), with 405 nm, 488 nm, 561 nm and 640 nm laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition was controlled by NIS Elements Advanced Research software (Nikon). Temperature-controlled motility assays were performed using a Linkam PE100-NIF inverted Peltier stage and T96 System Controller. Flow chamber slides were covered with a custom copper-plated aluminum fitting and empirical temperature of the flow chamber was monitored using a Type K thermocouple sensor probe.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!