Jeffamine ed 2003

Polymerizations were performed in a DAIHAN scientific shaking water bath. Jeffamine^® ED-2003 (Mn = 1900), acryloyl chloride, acrylonitrile, and polyvinylpyrrolidone (PVP_360,000) were purchased from Sigma-Aldrich (St. Louis, MI, USA), N-isopropylacrylamide (NIPAM) (99.0%) was purchased from Acros organics Co., Ltd. (St. Louis, MI, USA), and N, N’-methylenebisacrylamide (BIS) (97.0%) was purchased from Alfa Aesar Co., Ltd., Haverhill, UK. Cellulose Ester Spectra/Pore 1000 kDa cut-off molecular weight dialysis membrane was purchased from spectrum laboratories, Inc., Rancho Dominguez, CA, USA. Hydrodynamic sizes, size distributions, and zeta potential measurements were determined using a Zetasizer 3000 HSA (Malvern Instruments Ltd., Malvern, UK) operating with a 4 mW HeNe laser (632.8 nm), a detector positioned at a scattering angle of and a temperature-controlled jacket for the cuvette. The FTIR spectra were recorded on Thermo Nicolet iS10 with a smart omni transmission spectrometer. TGA was carried out using a Perkin Elmer Clarus 680/Clarus SQ 8C thermogravimetric analyzer. AFM was carried out using Bruker, AXS. XTT Assay Kit was purchased from Biological Industries, Beit Haemeq, Israel. Humidified incubator (Water-Jacketed, US Autoflow Automatic CO₂ Incubator manufactured by NuAire, Inc., Plymouth, MA, USA) was used for cell culture.