Grinder (GT203840, Tefal, United Kingdom), Rotamixer (Hook and Tucker Instruments Ltd., United Kingdom), Grant XB22 ultrasonic bath (Grant Instruments, United Kingdom), Centrifugator (Centrifuge 5804 R, Eppendorf, Germany), electronic balance (Sartorius CP64, Sartorius AG, Germany), Freeze dryer (ModulyoD Freeze Dryer, Thermo Fisher Scientific, United Kingdom), NMR tube (VWR international Ltd., United States), Bruker Advance 500 MHz spectrometer (Bruker, Germany), HPTLC plates silica gel 60 F 254 (Merck, Germany), Linomat 5 (CAMAG, Switzerland) coupled with a 100 μL syringe (CAMAG, Switzerland) and compressed air with 60–90 psi., Automatic Developing Chamber ADC 2 (CAMAG, Switzerland), TLC Visualizer (CAMAG, Switzerland) Microwell plate Nunclon 96 well (Thermo Scientific Nunc, United Kingdom), GalaxyB CO2 incubator (Scientific Laboratory Supplier Ltd., United Kingdom), water bath (LAUDA Aqualine AL 12, Germany), microscope (Olympus CK40 microscope, Japan), plate shaker (MS3 basic, IKA®, Germany) microplate reader (Infinite M200, Tecan, Switzerland), Multiwave Go (Anton Paar, Graz, Austria), ICP-OES SPECTROBLUE T1 (SPECTRO Analytical Instruments, Kleve, Germany).
100 μl syringe
Manufactured by CAMAG
Sourced in Switzerland
The 100 μL syringe is a laboratory equipment used for precise volume measurement and transfer of small liquid samples. It has a calibrated barrel and a plunger that allows users to accurately draw and dispense 100 microliters of liquid.
Automatically generated - may contain errors
Lab products found in correlation
Infinite m200, by Tecan (1 mentions)
Centrifuge 5804 r, by Eppendorf (1 mentions)
Automatic developing chamber adc 2, by CAMAG (1 mentions)
Linomat 5, by CAMAG (1 mentions)
Modulyod freeze dryer, by Thermo Fisher Scientific (1 mentions)
Hptlc plates silica gel 60 f 254, by Merck Group (1 mentions)
Tlc visualizer, by CAMAG (1 mentions)
Multiwave go, by Anton Paar (1 mentions)
Tlc scanner, by CAMAG (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Uv lamp, by Vilber (1 mentions)
3 protocols using 100 μl syringe
1
Quantitative Chemical Analysis Protocol
2
Quantitative TLC Analysis Using CAMAG System
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– The TLC Scanner (CAMAG) was controlled by WINCATS software. Scanning was performed in the absorbance mode with a 20 mm s−1 speed. A Linomat IV applicator was used for the band application with a 100μL syringe (CAMAG, Muttenz, Switzerland). And the source of radiation used was a deuterium lamp. It was operated with a band width of 5 mm, and 3 × 0.45 mm were selected as the slit dimensions. The chromatogram output data were obtained as integrated peak areas. HPTLC plates with a 0.25 mm coat of silica gel 60F254 (Merck, Germany) with dimensions of 15 × 20 cm were utilized.
– A Sonix TV ss-series ultra-sonicator (USA) was used.
– A Sonix TV ss-series ultra-sonicator (USA) was used.
3
HPTLC Method Development and Optimization
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In the developed HPTLC method, UV scanning was performed by using TLC scanner 3 densitometer (Camag, Muttenz, Switzerland). Samples were applied using TLC Linomat IV applicator equiped with 100 μL syringe (Camag, Muttenz, Switzerland). The instrument parameters were adjusted as follow: the slit dimensions were (6 × 0.3 mm), scanning speed was 20 mm/s, data resolution was 100 µm/ step, the used band width was 6 mm, and finally, chromatogram and integrated peak area were the result output.
The used stationary phase was (20 × 20 cm) HPTLC aluminum plates, coated with silica gel 60 F254 (Merck, Germany) with 0.25 mm thickness.
During method optimization, UV lamp (with wavelengths of 254 and 365 nm) (Vilber Lourmat, Marne La Vallee, Cedex, France) was used.
For dissolving the prepared samples, Sonix TV ss-series ultrasonicator (USA) was used.
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