All stocks were stored at −20°C. scOHCs were from Avanti (Alabama, US) and stored as 10 mM stocks in nitrogen flushed ethanol: 24OHC (#700061), 25OHC (#700019), 26OHC (#700021). Epirubicin (Cayman, UK Cat:12091) was stored at 10 mM in nuclease free water and protected from light. GSK2033 (ToCris, Abindon, UK – #5694) at 20 mM diluted in ETOH. ABC inhibitors: MK-571 (Cambridge Bioscience – Cat: 10029–1mg-CAY) and KO143 (Sigma – Cat: K2144–1mg) were diluted in DMSO, while Verapamil (Insight Biotechnology – Cat: sc-3590) was diluted in NFW; all at 10 mM. TaqMan assays (Thermo Fisher, Paisley, #4331182): LXRα [Hs00172885_m1] LXRβ [Hs01027215_g1], Pgp [Hs00184500_m1], ABCA1 [Hs01059137_m1], HPRT1 [Hs02800695_m1 ]. Origine trisilencer complexes (Maryland, US): LXRα #SR322981, LXRβ #SR305039). Antibodies: Pgp (Santa Cruz Biotech, CA, US - #sc73354), CYP27A1 (Abcam, Cambridge, UK - #ab126785), CYP46A1 (Abcam, Cambridge, UK - #ab198889), CH25H (Bioss, MA, US - #bs6480R), LXRα (R&D System, Minneapolis, US - #PP-PPZ0412–00), LXRβ (Active Motif, Carslbad, US - #61177). Antibody validation is described in detail in Supplementary Information .
Ch25h
Manufactured by Bioss Antibodies
Sourced in United States
CH25H is a laboratory instrument used for the detection and analysis of 25-hydroxycholesterol, a key metabolite in the cholesterol biosynthesis pathway. The core function of CH25H is to provide accurate and reproducible measurements of 25-hydroxycholesterol levels in biological samples, enabling researchers to study cholesterol metabolism and related biological processes.
Automatically generated - may contain errors
2 protocols using ch25h
1
Oxysterol Regulation of LXR Signaling
2
Cholesterol Regulation via LXRs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All stocks were stored at −20 °C. scOHCs were from Avanti (Alabama, US) and stored as 10 mM stocks in nitrogen flushed ethanol: 24OHC (#700061), 25OHC (#700019), 26OHC (#700021). Epirubicin (Cayman, UK Cat:12091) was stored at 10 mM in nuclease free water and protected from light. GSK2033 (ToCris, Abindon, UK – #5694) at 20 mM diluted in ETOH. ABC inhibitors: MK-571 (Cambridge Bioscience – Cat: 10029–1mg-CAY) and KO143 (Sigma – Cat: K2144–1mg) were diluted in DMSO, while Verapamil (Insight Biotechnology – Cat: sc-3590) was diluted in NFW; all at 10 mM. TaqMan assays (Thermo Fisher, Paisley, #4331182): LXRα [Hs00172885_m1] LXRβ [Hs01027215_g1], Pgp [Hs00184500_m1], ABCA1 [Hs01059137_m1], HPRT1 [Hs02800695_m1]. Origine trisilencer complexes (Maryland, US): LXRα #SR322981, LXRβ #SR305039). Antibodies: Pgp (Santa Cruz Biotech, CA, US - #sc73354), CYP27A1 (Abcam, Cambridge, UK - #ab126785), CYP46A1 (Abcam, Cambridge, UK - #ab198889), CH25H (Bioss, MA, US - #bs6480R), LXRα (R&D System, Minneapolis, US - #PP-PPZ0412-00), LXRβ (Active Motif, Carslbad, US - #61177). Antibody validation is described in detail in Supplementary Information.
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