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Anti phospho atm ser1981

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Anti-phospho-ATM Ser1981 is a primary antibody that recognizes the serine 1981-phosphorylated form of the ATM protein. ATM is a serine/threonine protein kinase that is activated in response to DNA double-strand breaks and plays a crucial role in the cellular DNA damage response pathway.

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Lab products found in correlation

Anti phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Agilent Technologies (2 mentions) Ecl system, by Thermo Fisher Scientific (2 mentions) Anti gapdh, by Merck Group (2 mentions) Anti phospho acc ser79, by Cell Signaling Technology (2 mentions) Anti phospho sirt 1 ser47, by Cell Signaling Technology (2 mentions) Anti p53, by Santa Cruz Biotechnology (2 mentions) Odyssey system, by LI COR (2 mentions) Anti flag, by Merck Group (2 mentions) Ku55933, by Merck Group (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti phospho chk2 thr68, by Cell Signaling Technology (2 mentions) Anti kap1, by Abcam (2 mentions) Anti nbs1, by GeneTex (2 mentions) Anti rad50, by GeneTex (2 mentions) Anti mre11, by GeneTex (2 mentions) Anti phospho ser824 kap1, by Fortis Life Sciences (2 mentions) Anti mbp, by Rockland Immunochemicals (2 mentions) Anti chk2, by GeneTex (2 mentions) Anti phospho ser15 p53, by Abcam (2 mentions)

Close spelling variants of this product

Phospho-ATM (4 citations) Anti-phospho-ATM (3 citations)

5 protocols using anti phospho atm ser1981

1

Western Blot Analysis of DNA Damage Signaling

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Whole cell protein extracts were prepared according to Laemmli (1970 (link)). The primary antibodies used were: anti-ATM (1:500), anti-phospho-ATM Ser1981 (1:500), anti-p300 (1:500) (Abcam, Cambridge, UK), anti-GAPDH (1:50000) (Millipore, Darmstadt, Germany); anti-p21WAF1/Cip1 (1:500) (Sigma-Aldrich, St. Louis, USA); anti-p53 (1:500), (Santa Cruz Biotechnology, Santa Cruz, USA); anti-ATR (1:500), anti-phospho-ATR Ser428 (1:500), anti-phospho-p53 Ser15 (1:250), anti-acetyl-p53 Lys382 (1:200), anti-SIRT1 (1:250), anti-phospho-SIRT1 Ser47 (1:250), anti-p38 MAPK (1:500), anti-phospho-p38 MAPK Thr180/Tyr182 (1:500), anti-phospho-MAPKAPK-2 Thr334 (1:500), anti-AMPKα (1:500), anti-phospho-AMPKα Thr172 (1:1000), anti-ACC (1:500), anti-phospho-ACC Ser79 (1:1000), anti-mTOR (1:500), anti-phospho-mTOR Ser2448 (1:500), anti-phospho-S6 Ser235/236 (1:1000) (Cell Signaling Technology, Denvers, USA), anti-Rb (1:250) (NeoMarkers, Fremont, USA). The respective proteins were detected after incubation with one of the horseradish peroxidase-conjugated secondary antibodies (1:2000) (Dako, Glostrup, Denmark), using an ECL system (Thermo Scientific, Rockford, USA), according to the manufacturer’s instructions.
Grabowska W., Mosieniak G., Achtabowska N., Czochara R., Litwinienko G., Bojko A., Sikora E, & Bielak-Zmijewska A. (2019). Curcumin induces multiple signaling pathways leading to vascular smooth muscle cell senescence. Biogerontology, 20(6), 783-798.
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2

Comprehensive Western Blot Analysis of Apoptosis and Cell Cycle Regulators

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Western blot assay was performed according to a previous study.
24 (link) Primary antibodies against various proteins were as follows: anti‐cleaved caspase‐3, anti‐cleaved caspase‐8, anti‐cleaved caspase‐9, anti‐BCL2, anti‐BCL‐XL, anti‐BAX were obtained from Cell Signaling Technology (CST, Beverly, USA); anti‐CDK4, anti‐CDK6, anti‐CyclinD1, anti‐Trip13, anti‐Ku70, anti‐Ku80, anti‐H2AX, anti‐phospho‐ H2AX (γ‐H2AX), anti‐CHK2, anti‐phospho‐CHK2 (Thr68), anti‐ATM, anti‐phospho‐ATM (Ser1981), and anti‐GAPDH were obtained from Abcam (Cambridge, MA, USA); anti‐β‐actin was obtained from Sigma (S. Louis, MO, USA).
Chang S., Xiao W., Xie Y., Xu Z., Li B., Wang G., Hu K., Zhang Y., Zhou J., Song D., Zhu H., Wu X., Lu Y., Shi J, & Zhu W. (2023). TI17, a novel compound, exerts anti‐MM activity by impairing Trip13 function of DSBs repair and enhancing DNA damage. Cancer Medicine, 12(23), 21321-21334.
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3

Western Blot Analysis of DNA Damage Response

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Whole cell protein extracts were prepared according to Laemmli [56 (link)]. The primary antibodies used were: anti-ATM (1:500), anti-phospho-ATM Ser1981 (1:500) (Abcam, Cambridge, UK); anti-GAPDH (1:50000) (Millipore, Darmstadt, Germany); anti-p53 (1:500) (Santa Cruz Biotechnology, Santa Cruz, USA); anti-p21 (1:500) (Sigma-Aldrich, St. Louis, USA); anti-phospho-p53 Ser15 (1:250), anti-sirt 1 (1:250), anti-phospho-sirt 1 Ser47 (1:250), anti-sirt 3 (1:500), anti-sirt 5 (1:500), anti-sirt 6 (1:1000), anti-sirt 7 (1:250) anti-AMPKα (1:500), anti-phospho-AMPKα Thr172 (1:1000), anti-phospho-ACC Ser79 (1:1000) (Cell Signaling Technology, Denvers, USA). The respective proteins were detected after incubation with the horseradish peroxidase-conjugated secondary antibodies (1:2000) (Dako, Glostrup, Denmark), using an ECL system (Thermo Scientific, Rockford, USA, according to the manufacturer's instructions.
Grabowska W., Suszek M., Wnuk M., Lewinska A., Wasiak E., Sikora E, & Bielak-Zmijewska A. (2016). Curcumin elevates sirtuin level but does not postpone in vitro senescence of human cells building the vasculature. Oncotarget, 7(15), 19201-19213.
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4

ATM/DNA-PK Regulation of DDR Signaling

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HCT116 Flp-In T-REx cells were treated with 10 μg/mL doxycycline (Dox) for 16 hr to induce the expression of homeodomain proteins. KU-55933 (EMD Millipore) and NU-7441 (R&D Pharmaceuticals; 3712) were used to treat cells for 1 hr for ATM or DNA-PK inhibition, respectively. DNA-damaging agents and hydrogen peroxide treatments were used to treat cells as indicated in figure legends. After treatment, cells were scraped into PBS and centrifuged at 800 × g for 2 min. Cells were lysed with cell lysis buffer, and lysates were clarified at 10,000 × g for 10 min. The protein concentrations of the lysates were quantified using Bradford assay, and protein levels were normalized for all samples. Primary antibodies used were anti-ATM (Santa Cruz), anti-phospho-Ser1981-ATM (Abcam), anti-Kap1 (Abcam; ab22553), anti-phospho-Ser824-Kap1 (Bethyl Laboratories; A300–767A), anti-Nbs1 (Genetex; GTX70224), anti-Rad50 (Genetex; GTX70228), anti-Mre11 (Genetex; GTX70212), anti-MBP (Rockland; 200–401–385), anti-Flag (Sigma; A1205), anti-β-actin (Cell Signaling; 4970), anti-Chk2 (Genetex; GTX70295), anti-phospho-Thr68-Chk2 (Cell Signaling; 2661S), anti-phospho-Ser15-p53 (Abcam; ab1431), and anti-phospho-Ser15-p53 (mouse) (Assay Biotech; A7180). Alexa Fluor 68-anti-rabbit and IRDye 800 anti-mouse were the secondary antibodies used. Membranes were scanned and quantified using the Odyssey system (Li-Cor).
Johnson T.E., Lee J.H., Myler L.R., Zhou Y., Mosley T.J., Yang S.H., Uprety N., Kim J, & Paull T.T. (2018). Homeodomain Proteins Directly Regulate ATM Kinase Activity. Cell reports, 24(6), 1471-1483.
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5

ATM/DNA-PK Regulation of DDR Signaling

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HCT116 Flp-In T-REx cells were treated with 10 μg/mL doxycycline (Dox) for 16 hr to induce the expression of homeodomain proteins. KU-55933 (EMD Millipore) and NU-7441 (R&D Pharmaceuticals; 3712) were used to treat cells for 1 hr for ATM or DNA-PK inhibition, respectively. DNA-damaging agents and hydrogen peroxide treatments were used to treat cells as indicated in figure legends. After treatment, cells were scraped into PBS and centrifuged at 800 × g for 2 min. Cells were lysed with cell lysis buffer, and lysates were clarified at 10,000 × g for 10 min. The protein concentrations of the lysates were quantified using Bradford assay, and protein levels were normalized for all samples. Primary antibodies used were anti-ATM (Santa Cruz), anti-phospho-Ser1981-ATM (Abcam), anti-Kap1 (Abcam; ab22553), anti-phospho-Ser824-Kap1 (Bethyl Laboratories; A300–767A), anti-Nbs1 (Genetex; GTX70224), anti-Rad50 (Genetex; GTX70228), anti-Mre11 (Genetex; GTX70212), anti-MBP (Rockland; 200–401–385), anti-Flag (Sigma; A1205), anti-β-actin (Cell Signaling; 4970), anti-Chk2 (Genetex; GTX70295), anti-phospho-Thr68-Chk2 (Cell Signaling; 2661S), anti-phospho-Ser15-p53 (Abcam; ab1431), and anti-phospho-Ser15-p53 (mouse) (Assay Biotech; A7180). Alexa Fluor 68-anti-rabbit and IRDye 800 anti-mouse were the secondary antibodies used. Membranes were scanned and quantified using the Odyssey system (Li-Cor).
Johnson T.E., Lee J.H., Myler L.R., Zhou Y., Mosley T.J., Yang S.H., Uprety N., Kim J, & Paull T.T. (2018). Homeodomain Proteins Directly Regulate ATM Kinase Activity. Cell reports, 24(6), 1471-1483.
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