Metformin

Manufactured by R&D Systems

Sourced in United Kingdom

Metformin is a chemical compound commonly used in laboratory research. It serves as a key regulator of cellular energy metabolism and has applications in the study of various biological processes.

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Lab products found in correlation

Doxycycline, by Merck Group (1 mentions) Quinine, by Merck Group (1 mentions) Barium chloride, by Merck Group (1 mentions) Mcc950, by Merck Group (1 mentions) Iberiotoxin, by Bio-Techne (1 mentions) Aicar, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Ascorbate, by Merck Group (1 mentions) Wortmannin, by InvivoGen (1 mentions)

2 protocols using metformin

Modulation of NLRP3 Inflammasome Activation

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HEK-293T and A549 cells were transfected with plasmids. After 2h, different chemical reagents were added to medium. Chemical reagents included NLRP3 inhibitor MCC950 (0.1–5 μM) (Sigma Aldrich, Cat. 538120), Quinine (10 μM) (Sigma Aldrich, Cat. 145904), Barium chloride (2 mM) (Sigma Aldrich, Cat. 342920), Tetraethylammonium chloride (5 mM) (Tocris Bioscience, Cat. 306850), Iberiotoxin (1.0 μM) (Tocris Bioscience, Cat. 1086100U), Doxycycline (Sigma Aldrich, Cat. D3447), Ibrutinib (R&D, Cat. 6813), and Metformin (R&D, Cat. 2864). All chemicals were dissolved with DMSO or sterile water.

Xu H., Akinyemi I.A., Chitre S.A., Loeb J.C., Lednicky J.A., McIntosh M.T, & Bhaduri-McIntosh S. (2022). SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathway. Virology, 568, 13-22.

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Immune Modulation by Fungal β-Glucan

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Candida albicans β-1,3-(D)-glucan (β-glucan) were kindly provided by Professor David Williams. Reagents used were as follows: LPS (E. coli 0B5/B5, Sigma chemical company, Diegem, Belgium), rapamycin (Sigma, R0395), metformin (R&D, AF 1730, Abingdon, UK), AICAR (Sigma, A9978), ascorbate (Sigma, A4034), wortmannin (InvivoGen, tlrl-wtm, Toulouse, France). C. albicans ATCC MYA-3573 (UC 820) were heat-inactivated for 30 min at 95°C.

Cheng S.C., Quintin J., Cramer R.A., Shepardson K.M., Saeed S., Kumar V., Giamarellos-Bourboulis E.J., Martens J.H., Rao N.A., Aghajanirefah A., Manjeri G.R., Li Y., Ifrim D.C., Arts R.J., van der Meer B.M., Deen P.M., Logie C., O’Neill L.A., Willems P., van de Veerdonk F.L., van der Meer J.W., Ng A., Joosten L.A., Wijmenga C., Stunnenberg H.G., Xavier R.J, & Netea M.G. (2014). mTOR/HIF1α-mediated aerobic glycolysis as metabolic basis for trained immunity. Science (New York, N.Y.), 345(6204), 1250684.

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