1 undecanol

For the proposed experiments, four NSAIDs, namely, diclofenac sodium salt (DIC), ibuprofen (IBU), ketoprofen (KET), and naproxen (NAP), together with four estrogens, namely, estrone (E1), 17β-estradiol (E2), 17α-ethynylestradiol (EE2), and estriol (E3), and one glucocorticoid, namely, hydrocortisone (HCOR), all with purity levels >98%, were purchased from Sigma-Aldrich (Steinheim, Germany). The molecular structure and some physico-chemical properties of all target compounds are presented in the Supplementary Materials (Table S6).
Stock standard solutions of the individual compounds, at a concentration of 1000 mg/L each, were prepared in Acetonitrile for ibuprofen, ketoprofen, and naproxen, while diclofenac and the hormones were prepared in methanol. All prepared stock standards were stored at 4 °C in the dark until analysis. Acetonitrile and methanol of HPLC grade, 1-undecanol, NaCl, ortho-phosphoric acid, acetic acid, and n-hexane were all purchased from Merck (Darmstadt, Germany).
Milli-Q water was prepared using a Milli-Q-Plus ultrapure water system (Millipore, Milford, MA, USA). An Eppendorf centrifuge, model 5804 R (Eppendorf, Wien, Austria), was used for the centrifugation of the samples.

Chromatographic quality organic solvents, namely acetonitrile and methanol were obtained from Chem-Lab (Zedelgem, Belgium). Chloroform, carbon tetrachloride, 1-undecanol and 1-octanol were purchased from Sigma Aldrich (St. Louis, MO, USA). The ILs 1-hexyl-3-methylimidazolium hexafluorophosphate (C₁₀H₁₉F₆N₂P) [HMIM][PF6], lithium-imide bis(trifluoromethylsulfonyl)imide (LiC₂F₆NO₄S₂) [LiTFSI] and 1-hexyl-3-methylimidazolium chloride (C₁₀H₁₉ClN₂) [HMIM Cl], were supplied by IOLITEC (Heilbronn, Germany). Other reagents, including formic acid and sodium chloride, were acquired from Sigma. Anhydrous magnesium sulfate from Análisis Vínicos (Tomelloso, Spain), hydrochloric acid from Riedel-de-Häen (Wunstarfer, Germany) and phosphoric acid from Panreac Química S.A (Barcelona, Spain) were also used. Water was previously purified using a Milli-Q system (Millipore, Bedford, MA, USA).
The cyanotoxin and phycotoxin standards were obtained from Cifga S.A. (Lugo, Spain). The standard solutions of cyanotoxins—microcystine LR (MC-LR) and RR (MC-RR) and nodularin (NOD)—were supplied at 10 µg g⁻¹, while okadaic acid (OA) was supplied at 16 µg g⁻¹ and domoic acid (DA) at 40 µg g⁻¹. All were stored in the dark at −20 °C, and standard working solutions were prepared daily diluting the toxins with ultrapure water.

Repaglinide (99.8%) and nateglinide (99.2%) were kindly supplied by Sigma Pharmaceutical Industries (Quwaysna, Egypt). Methanol (HPLC grade), acetonitrile (HPLC grade) and potassium dihydrogen phosphate were purchased from Merck (Darmstadt, Germany). 1-Octanol, 1-decanol, 1-undecanol and 1-dodecanol (analytical grade) were purchased from Sigma Aldrich (Darmstadt, Germany). Glycerol, propylene glycol, sodium hydroxide, and phosphoric acid were purchased from Piochem (6th of October, Egypt). Distilled water was used for the preparation of aqueous solutions. Drug-free human plasma from healthy volunteers (Blood Bank, Benha University Hospitals, Benha, Egypt) was used for the application of the vortex-assisted DLLME method. The plasma samples, stored at − 20 °C were thawed to room temperature before use.

All chemicals used are of at least analytical reagent grade. Cobalt chloride hexahydrate (CoCl₂·6H₂O), metal ions studied for interferences, pyrocatechol violet and nitric acid were obtained from Carlo-Erba, France. 1-Undecanol, 1-dodecanol, dodecyltrimethyl ammonium bromide (DTAB), trimethyltetradecyl ammonium bromide (TTAB), and cethyltrimethyl ammonium bromide (CTAB) were purchased from Sigma-Aldrich (USA). 1-Octanol was obtained from Panreac (Spain). Methanol (HPLC grade) and acetonitrile (HPLC grade) were obtained from Merck (Germany). Acetone was purchased from Q-rac (Malaysia). Sodium sulphate anhydrous was purchased from Fluka (Japan). Sodium chloride, sodium hydrogen phosphate and sodium dihydrogen phosphate were purchased from Ajax Finechem (Australia). Aluminium chloride was obtained from Ajax Finechem Pty Ltd (New Zealand). All solutions were prepared using deionized water with the resistivity of 18.2 MΩ cm from RiOs™ Type I Simplicity 185 (Millipore Waters, USA). Stock solution of standard Co(ii) 100 mg L⁻¹ (or 1.695 mmol L⁻¹) was prepared and used throughout.

All chemicals and reagents used in this work were of analytical grade. Five triazole fungicides (myclobutanil (MCBT), triadimefon (TDF), tebuconazole (TBZ), hexaconazole (HCZ), and diniconazole (DCZ)) from Dr. Ehrenstorfer GmbH (Augsburg, Germany)were used. Methanol (Merck, Darmstadt, Germany) was used to prepare the stock solution of each fungicide (1000 mg L⁻¹) and stored in refrigerator at 4 °C under light protection until analysis. HPLC-grade Methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). 1-Undecanol and 1-dodecanol were purchase from Sigma-Aldrich (Darmstadt, Germany). Deionized water with the resistivity of 18.2 MΩ.cm was obtained from a Type 1 Simplicity^® ultrapure water system (Merck, Darmstadt, Germany). All solutions were filtered through a 0.45 μm nylon membrane filter before injected into the HPLC system.