Foxp3yfp cre mice

C57BL/6 mice and BALB/c mice were purchased from CLEA (Tokyo, Japan). RAG2^−/− mice and Foxp3^YFP−Cre mice on a C57BL/6 background were purchased from Jackson Laboratory (Bar Harbor, ME). Foxp3^hCD2 mice on a BALB/c background were described previously (21 (link)). All mice were housed in microisolator cages under specific pathogen-free conditions, and all experiments were performed according to the guidelines of Chiba University established by Chiba University for experiments in animals, which conform to the “Guide for the Care and Use of Laboratory Animals” published by the US National Institutes of Health (NIH Publication, 8th Edition, 2011).

Male C57BL/6J and CD45.1 mice aged 8–12 weeks were purchased from the Model Animal Research Center of Nanjing University (Nanjing, China). Foxp3‐GFP mice were kindly provided by Dr. Rudensky AY (Howard Hughes Medical Institute, Immunology Program, and Ludwig Center, Memorial Sloan Kettering Cancer Center, New York, NY). ST2^–/– mice on a C57BL/6J background were obtained from Dr. Andrew McKenzie (Medical Research Council Laboratory of Molecular Biology, University of Cambridge, Cambridge, U.K.). KikGR/B6‐ROSA transgenic mice were purchased from RIKEN BioResource Research Center (Tokyo, Japan). Depletion of regulatory T cells (DEREG) and Foxp3^YFP‐cre mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Tff1^flox/flox mice were established and Tff1^–/– mice were purchased by Cyagen (Suzhou, China). Treg‐specific Tff1 knockout mice were created by crossing Tff1^flox/flox mice with Foxp3^YFP‐cre mice. The incidence of AAA in male mice is higher than in female mice,^[44, ^45] so only male mice were used throughout this study. All animal studies were approved by the Animal Care and Utilization Committee of Huazhong University of Science and Technology. Experiments were conducted in accordance with NIH guidelines. Animals were randomly assigned to experimental groups.

C57BL/6 mice were purchased from The Jackson Laboratory. Blimp-1–YFP (commercially available at Jackson, stock number 008828), Foxp3-RFP (commercially available at Jackson, stock number 008374) (43 (link)), and Blimp-1^fl/fl mice (commercially available at Jackson, stock number 008100) (44 (link)) were donated by A. Poholek (University of Pittsburgh). IL-10^fl/fl mice were donated by D. Vignali (University of Pittsburgh). Foxp3-YFP-Cre mice were purchased from The Jackson Laboratory (stock number 016959) (18 (link)). All experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee. All mice used in experiments were age-matched littermate males between 26 and 28 weeks of age. Male mice were used due to the hormonal fluctuations that occur in female mice. Where indicated, mice were fed either SFD (standard chow) or HFD (60% kcal fat, Teklad, Envigo, TD06414) from 8 weeks of age until 26–28 weeks of age. Male animals were assigned to groups of 3–7 mice per experiment where possible, and at least 2 independent experiments were performed throughout the study. Mice were bred and housed in specific pathogen–free conditions in accordance with the Institutional Animal Care and Use Guidelines of the University of Pittsburgh. Mice were maintained at approximately 22°C room temperature as standard.

We purchased WT BALB/c and WT C57BL/6 mice; B6/Rag−/− mice lacking mature T and B cells^{10 (link)}; DEREG/B6 mice engineered to express the diphtheria toxin receptor plus a green fluorescent protein (DTR-eGFP) within fully functional Foxp3 + CD4 + Treg cells^{20 (link)}; Foxp3^YFP-Cre mice^{36 (link)} and CXCR4^fl/fl mice^{37 (link)} (both strains on the B6 background) from The Jackson Laboratory. All mice were used at 8–12 weeks of age. Animal study protocols were approved and undertaken in accordance with the regulations and guidelines of the Institutional Animal Care and Use Committee of The Children’s Hospital of Philadelphia (19-001052). Experiments were performed with age‐ and sex‐matched mice and using animals that were littermates or were maintained in the same room and/or were co‐housed within the same cages for >2 weeks to limit potential effects of microbiome differences.

C57BL/6J mice were purchased from CLEA Japan. Foxp3-DTR-eGFP (FDG) mice (12 (link)) and Foxp3^YFP–Cre mice (13 (link)) were purchased from the Jackson Laboratory. Areg^fl/fl mice were generated by inserting Loxp3 sites flanking exons 3 and 4 of the murine Areg gene using the CRISPR/Cas9 system. These mice were crossed with Foxp3^YFP–Cre mice. All mice used had a C57BL/6J background and were maintained under specific-pathogen-free conditions. All animal experiments were approved by the Ethical Committee of Institute for Frontier Life and Medical Sciences and Graduate School of Medicine, Kyoto University. All relevant experiments were performed in accordance with the institutional guidelines.