Sections were analyzed and images were acquired using a Zeiss LSMDUO confocal laser scanning microscope with a META module (Carl Zeiss MicroImaging GmbH, Germany) microscope, the LSM700 AxioObserver. The Zen 2011 (LSM 700, Zeiss software, Oberkochen, Germany) built-in “colocalization view” was used to highlight the expression of both antibodies’ signals [76 (link),77 (link),78 (link)], in order to produce a “colocalization” signal, along with the display profile, the scatter plot and fluorescent signal measurements. Each image was rapidly acquired to minimize photodegradation.
Zen 2011 lsm 700
Manufactured by Zeiss
Sourced in Germany
The Zen 2011 (LSM 700) is a laser scanning microscope produced by Zeiss. It is designed for high-resolution imaging and analysis of samples. The core function of the Zen 2011 (LSM 700) is to provide users with advanced imaging capabilities through the use of laser scanning technology.
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2 protocols using zen 2011 lsm 700
1
Confocal Microscopy for Protein Colocalization
2
Confocal Microscopy Imaging Protocol
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Processed specimens were viewed using a LSM 700 Zeiss confocal microscope. The sections were analyzed, and images acquired using a Zeiss LSMDUO confocal laser scanning microscope with META module (Carl Zeiss, Micro Imaging, GmbH, Jena, Germany). Zen 2011 (LSM 700, Zeiss software) built in colocalization view was used to highlight the expression of both antibody signals in order to produce a co-labeling signal. Digital images were cropped, and the figure montage was prepared by the use of Adobe Photoshop 7.0 (Adobe System, San Jose, CA, USA).
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