4 tert octylphenol

Manufactured by Merck Group

Sourced in United States

4-tert-octylphenol is a chemical compound used in various laboratory applications. It serves as a versatile reagent and intermediate in organic synthesis. The core function of this product is to facilitate specific chemical reactions and processes within a controlled laboratory setting.

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Octylphenol (3 citations) 4-octylphenol (2 citations)

6 protocols using 4 tert octylphenol

Synthesis and Characterization of Novel Microporous Materials

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Zinc nitrate hexahydrate (98%), 2-methylimidazole (99%), sodium hydroxide (98%), methanol (HPLC grade, 99.9%), ethylenediaminetetraacetic acid (99%), sodium chloride (99%), potassium dihydrogen phosphate (99%), bisphenol-A (97%), 4-tert-butylphenol (99%), 4-tert-octylphenol (97%), and 4-nonylphenol (99%), were purchased from Sigma-Aldrich. Rhodamine B (≥95%) was purchased from VWR. Potassium chloride (99.8%) was purchased from AJAX Chemicals. Disodium hydrogen phosphate (99%) was purchased from Merck, Rahway, NJ, USA.

Moore E.W, & Maya F. (2023). ZIF-8@Rhodamine B as a Self-Reporting Material for Pollutant Extraction Applications. Nanomaterials, 13(5), 842.

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Characterization of Endocrine Disruptors in Artemia

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The organic solvents, methanol and absolute ethanol, were supplied by Fisher Scientific (Loughborough, UK), while acetonitrile was supplied by VWR Chemicals (Paris, France). Trifluoroacetic acid (TFA) and sodium hypochlorite solution 10% w/v were purchased from AppliChem GmbH (Darmstadt, Germany). The sea salt used as the Artemia culture medium was purchased from Instant Ocean (Blacksburg, VA, USA). Artemiafranciscana cysts were kindly supplied by INVE Aquaculture (INVE HELLAS SA, 93, Kyprou str., 16451 Argyroupoli, Athens, Greece). The C₁₈ sorbent material, columns, and frits for SPE were supplied by Grace Davison Discovery Sciences (Bannockburn, IL, USA).
The internal standard 4-n-Octylphenol and Bisphenol-A were supplied by Alfa Aesar (Karlsruhe, Germany), while 4-tert-Octylphenol was obtained from Sigma-Aldrich (Germany). The investigated compounds are presented in Table 1.

Giamaki D., Dindini K., Samanidou V.F, & Touraki M. (2022). Simultaneous Quantification of Bisphenol-A and 4-Tert-Octylphenol in the Live Aquaculture Feed Artemia franciscana and in Its Culture Medium Using HPLC-DAD. Methods and Protocols, 5(3), 38.

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Xenoestrogen Characterization and Regulation

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17β-estradiol (≥98.0%), 17α-ethinylestradiol (≥98.0%), bisphenol A (≥99.0%), 4-tert-octylphenol (≥99.0%), and acetonitrile HPLC grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Water HPLC grade was acquired from Tedia Company (Fairfield, OH, USA). The main characteristics of the studied compounds are shown in Table 1.Table 1

Characteristics of the studied xenoestrogens.

Table 1

	Chemical formula	Molecular mass (g mol⁻¹)	Log K_ow	Solubility (mg L⁻¹)	Use	MPCa proposed by the European Parliament
E2	C₁₈H₂₄O₂	272.38	4.0	28.0	Natural estrogen	0.001 µg L⁻¹[3]
EE2	C₂₀H₂₄O₂	296.40	3.6	11.3	Synthetic estrogen	0.035 ng L⁻¹[4]
BPA	C₁₅H₁₆O₂	228.29	3.3	120	Plasticizer	0.01 µg L⁻¹[3]
4TOP	C₁₄H₂₂O	206.32	5.2	5.0	Surfactant	2 µg L⁻¹[5]

Maximum permissible concentration in surface water.

López-Velázquez K., Guzmán-Mar J.L., Montalvo-Herrera T., Mendiola-Alvarez S.Y, & Villanueva-Rodríguez M. (2022). Development of an HPLC-DAD method for the simultaneous analysis of four xenoestrogens in water and its application for monitoring photocatalytic degradation. MethodsX, 9, 101789.

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Expression and Purification of ER-α LBD

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The wild-type estrogen receptor α ligand binding domain encoding gene was synthesized and cloned into pET21a(+) by Genscript (Piscataway, NJ, USA). The QuikChange Site-Directed-Mutagenesis Kit was purchased by Qiagen (Hilden, Germany), while primers and sequencing were done by Primm (Milan, Italy).
The Bacto Yeast Extract and Bacto Tryptone for the Luria Bertani (LB) medium were from BD Biosciences (Franklin Lakes, NJ, USA). Rosetta 2(DE3)pLysS Competent Cells come from Merck.
Isopropyl β-D-1-thiogalactopyranoside (IPTG), Tris-HCl, NaCl, β-mercaptoethanol (β-Me), 3-(1-pyridinio)-1-propanesulfonate (NDSB) and the test compounds 17β-estradiol, 17α-ethynilestradiol, bisphenol-A, tamoxifen, 4-nonylphenol and 4-tert-octylphenol were supplied by Sigma Aldrich.
Nickel HiLoad Column and the AKTA System purifier are from GE Healthcare. Life Technologies supplied the PolarScreen Estrogen Receptor-α Competitor Green Assay.

Ferrero V.E., Pedotti M., Chiadò A., Simonelli L., Calzolai L., Varani L, & Lettieri T. (2014). Rational Modification of Estrogen Receptor by Combination of Computational and Experimental Analysis. PLoS ONE, 9(7), e102658.

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Phthalate and Alkylphenol Analysis

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Bis (2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), 4-nonylphenol (NP), 4-tert-octylphenol (OP) and DMSO (dimethyl sulfoxide) were purchased from Sigma-Aldrich Co, (USA). Ethanol was acquired from Winkler (Chile).

Buñay J., Larriba E., Moreno R.D, & del Mazo J. (2017). Chronic low-dose exposure to a mixture of environmental endocrine disruptors induces microRNAs/isomiRs deregulation in mouse concomitant with intratesticular estradiol reduction. Scientific Reports, 7, 3373.

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Endocrine-Disrupting Chemicals Exposure Study

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Lipolysaccharides (LPS) from Escherichia coli 0111:B4, phorbol 12-myristate 13-acetate (PMA), fetal bovine serum (FBS), Bisphenol A, di-ethylhexyl-phthalate, dibutylphthalate, 4-tert-octylphenol and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma (Saint Quentin en Fallavier, France). RPMI medium, L-glutamine (200 mM) and penicillin-streptomycin solution (10,000 U/ml, 10,000 μg/ml) were purchased from Invitrogen (Cergy Pontoise, France). Structures of EDCs tested in the present work are shown in S1 Fig.

Couleau N., Falla J., Beillerot A., Battaglia E., D’Innocenzo M., Plançon S., Laval-Gilly P, & Bennasroune A. (2015). Effects of Endocrine Disruptor Compounds, Alone or in Combination, on Human Macrophage-Like THP-1 Cell Response. PLoS ONE, 10(7), e0131428.

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