Western blotting was performed as previously reported [61 (link), 62 (link)]. The antibodies used were rabbit polyclonal anti-phospho-Ser473-Akt (#9271, 1:1000, Cell Signaling Technology, Danvers MA, USA), rabbit monoclonal anti-Akt (#4691, 1:1000, Cell Signaling Technology), rabbit polyclonal phospho-Ser9-GSK3β (#9336, 1:1000, Cell Signaling Technology), rabbit monoclonal GSK3β (#9315, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-phospho-Thr202/Tyr204-ERK1/2 (#9101, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-ERK1/2 (#9102, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-phospho-Ser217/221-MEK1/2 (#9121, Cell Signaling Technology), rabbit polyclonal anti-MEK1/2 (#9122, Cell Signaling Technology), rabbit polyclonal anti-p16 (10883-1-AP, 1:500, Proteintech, Rosemont, IL, USA), rat monoclonal anti-p19 (sc-32748, 1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit polyclonal anti-p21 (ab7960, Abcam, Cambridge, UK), goat polyclonal anti-IGFBP5 (sc-6006, 1:200, Santa Cruz Biotechnology), mouse monoclonal anti-FLAG (F3165, 1:1000, Sigma Aldrich; Merck KGaA, Darmstadt, Germany), mouse monoclonal anti-β-actin (010-27841, 1:10000, Fujifilm Wako Pure Chemical Corporation, Osaka, Japan), and mouse monoclonal anti-GAPDH (G8795, 1:10000, Sigma Aldrich, St. Louis, MO, USA).
Rabbit polyclonal anti mek1 2
Manufactured by Cell Signaling Technology
Sourced in United States, United Kingdom
Rabbit polyclonal anti-MEK1/2 is a laboratory reagent that recognizes MEK1 and MEK2 proteins. It can be used to detect and analyze these proteins in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit monoclonal gsk3β, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti gapdh, by Merck Group (1 mentions)
Rabbit monoclonal anti akt, by Cell Signaling Technology (1 mentions)
Rabbit polyclonal anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti flag, by Merck Group (1 mentions)
Mouse monoclonal anti parkin, by Santa Cruz Biotechnology (1 mentions)
Rabbit monoclonal anti lc3b, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti tom20, by Santa Cruz Biotechnology (1 mentions)
Mouse monoclonal anti ha, by Fortrea (1 mentions)
2 protocols using rabbit polyclonal anti mek1 2
1
Antibody-Based Western Blotting Methodology
2
Mitochondrial Dynamics Protein Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and chemicals were purchased from Sigma-Aldrich unless otherwise stated. Antibodies were as follows: rabbit polyclonal anti-PKAβ catalytic (Catβ), mouse monoclonal anti–mouse PKAα catalytic (Catα), monoclonal anti-PKAIIα regulatory (RIIα), mouse monoclonal anti-PKAIα regulatory (RIα), mouse monoclonal anti-PKAIIβ regulatory (RIIβ), rabbit monoclonal anti-TOM20, mouse monoclonal anti-TOM20, mouse monoclonal anti-Parkin, (Santa Cruz Biotechnology, Inc.); mouse monoclonal anti-DRP1 (BD); rabbit polyclonal anti-PINK1, rabbit polyclonal anti-AKAP1 (Novus Biologicals); mouse monoclonal anti-PDH E1α (ProteinTech); mouse monoclonal anti-β-actin, mouse monoclonal anti-MFN1, (Abcam); mouse monoclonal anti-HA (Covance); rabbit polyclonal anti-phospho-637 DRP1, rabbit polyclonal anti-Prohibitin, rabbit monoclonal anti-LC3B, rabbit polyclonal anti-MEK1/2 (Cell Signaling Technology); complex IV subunit II was a gift from J.-W. Taanman (University College London, London, England, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!