Ribo fluorescent in situ hybridization

Manufactured by RiboBio

Sourced in China

RiboTM Fluorescent In Situ Hybridization is a laboratory equipment product designed for the detection and localization of specific RNA molecules within cells or tissue samples. The core function of this product is to enable the visualization and analysis of the expression and distribution of target RNA sequences using fluorescence microscopy.

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Lab products found in correlation

Ribo lncrna fish probe mix, by RiboBio (1 mentions)

2 protocols using ribo fluorescent in situ hybridization

Subcellular Localization of linc02042

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The subcellular localization of linc02042 was determined by Nuclear-Cytoplasmic isolation and fluorescence in situ hybridization (FISH) assays, which were respectively performed by using the Cytoplasmic & Nuclear RNA Purification (Norgen Biotek Corp, ON, CAN) and RiboTM Fluorescent In Situ Hybridization (RiboBio, Guangzhou, China) kits in accordance with the instructions from manufacturers.

Du J., Zhang G., Qiu H., Yu H, & Yuan W. (2020). A novel positive feedback loop of linc02042 and c-Myc mediated by YBX1 promotes tumorigenesis and metastasis in esophageal squamous cell carcinoma. Cancer Cell International, 20, 75.

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Localization of lncRNA LINC317.5 in HepG2 cells

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We observed the distribution of lncRNA in the HepG2 by fluorescence in situ hybridization (FISH) Kit (Ribo^TM lncRNA FISH Probe Mix and Ribo^TM Fluorescent In Situ Hybridization; RiboBio, China). We used RiboBio to design and synthesize Cy3-labeled LINC317.5 probes, U6 probes, and 18 S probes. Both U6 and 18 S were the internal reference, U6 was almost all located in the nucleus while 18 S was almost all located in the cytoplasm.

Yang Y., Sun M., Yan S., Yao N., Li X., Wu C., Wu Z., Wang F., Cui W, & Li B. (2024). LINC317.5 as a novel biomarker for hypertriglyceridemia in normal glucose metabolism. Cell Death Discovery, 10, 194.

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